Transcription initiation at a consensus bacterial promoter proceeds via a ‘bind-unwind-load-and-lock’ mechanism by Abhishek Mazumder, Richard H Ebright, Achillefs N Kapanidis

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T7 RNA polymerase functions in vitro without clustering. by Kieran Finan, Joseph P Torella, Achillefs N Kapanidis, Peter R Cook

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DNA polymerase conformational dynamics and the role of fidelity-conferring residues: Insights from computational simulations by Massimiliano eMeli, Marko eSustarsic, Timothy D Craggs, Achillefs N Kapanidis, Giorgio eColombo

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Single-molecule imaging of UvrA and UvrB recruitment to DNA lesions in living Escherichia coli by Mathew Stracy, Marcin Jaciuk, Stephan Uphoff, Achillefs N. Kapanidis, Marcin Nowotny, David J. Sherratt, Pawel Zawadzki

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The Localization and Action of Topoisomerase IV in Escherichia coli Chromosome Segregation Is Coordinated by the SMC Complex, MukBEF by Pawel Zawadzki, Mathew Stracy, Katarzyna Ginda, Katarzyna Zawadzka, Christian Lesterlin, Achillefs N. Kapanidis, David J. Sherratt

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Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA by Christof Hepp, Nicolas Shiaelis, Nicole C. Robb, Alison Vaughan, Philippa C. Matthews, Nicole Stoesser, Derrick Crook, Achillefs N. Kapanidis

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Deep learning and single-cell phenotyping for rapid antimicrobial susceptibility detection in Escherichia coli by Alexander Zagajewski, Piers Turner, Conor Feehily, Hafez El Sayyed, Monique Andersson, Lucinda Barrett, Sarah Oakley, Mathew Stracy, Derrick Crook, Christoffer Nellåker, Nicole Stoesser, Achillefs N. Kapanidis

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Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria by David Dulin, David L. V. Bauer, Anssi M. Malinen, Jacob J. W. Bakermans, Martin Kaller, Zakia Morichaud, Ivan Petushkov, Martin Depken, Konstantin Brodolin, Andrey Kulbachinskiy, Achillefs N. Kapanidis

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Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes by Hannah L Klein, Kenny K.H. Ang, Michelle R. Arkin, Emily C. Beckwitt, Yi-Hsuan Chang, Jun Fan, Youngho Kwon, Michael J. Morten, Sucheta Mukherjee, Oliver J. Pambos, Hafez el Sayyed, Elizabeth S. Thrall, João P. Vieira-da-Rocha, Quan Wang, Shuang Wang, Hsin-Yi Yeh, Julie S. Biteen, Peter Chi, Wolf-Dietrich Heyer, Achillefs N. Kapanidis, Joseph J. Loparo, Terence R. Strick, Patrick Sung, Bennett Van Houten, Hengyao Niu, Eli Rothenberg

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FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices by Eitan Lerner, Anders Barth, Jelle Hendrix, Benjamin Ambrose, Victoria Birkedal, Scott C Blanchard, Richard Börner, Hoi Sung Chung, Thorben Cordes, Timothy D Craggs, Ashok A Deniz, Jiajie Diao, Jingyi Fei, Ruben L Gonzalez, Irina V Gopich, Taekjip Ha, Christian A Hanke, Gilad Haran, Nikos S Hatzakis, Sungchul Hohng, Seok-Cheol Hong, Thorsten Hugel, Antonino Ingargiola, Chirlmin Joo, Achillefs N Kapanidis, Harold D Kim, Ted Laurence, Nam Ki Lee, Tae-Hee Lee, Edward A Lemke, Emmanuel Margeat, Jens Michaelis, Xavier Michalet, Sua Myong, Daniel Nettels, Thomas-Otavio Peulen, Evelyn Ploetz, Yair Razvag, Nicole C Robb, Benjamin Schuler, Hamid Soleimaninejad, Chun Tang, Reza Vafabakhsh, Don C Lamb, Claus AM Seidel, Shimon Weiss

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