NgAgo

NgAgo is a single-stranded DNA (ssDNA)-guided Argonaute endonuclease, an acronym for ''Natronobacterium'' ''gregoryi'' Argonaute. NgAgo binds 5′ phosphorylated ssDNA of ~24 nucleotides (gDNA) to guide it to its target site and will make DNA double-strand breaks at the gDNA site. Like the CRISPR/Cas system, NgAgo was reported by Chunyu Han et al. to be suitable for genome editing, but this has not been replicated. In contrast to Cas9, the NgAgo–gDNA system does not require a protospacer adjacent motif (PAM). Provided by Wikipedia