Creation of Novel Protein Variants with CRISPR/Cas9-Mediated Mutagenesis: Turning a Screening By-Product into a Discovery Tool. by Katherine F Donovan, Mudra Hegde, Meagan Sullender, Emma W Vaimberg, Cory M Johannessen, David E Root, John G Doench

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Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression by Joseph Rosenbluh, Han Xu, William Harrington, Stanley Gill, Xiaoxing Wang, Francisca Vazquez, David E. Root, Aviad Tsherniak, William C. Hahn

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Chronos: a cell population dynamics model of CRISPR experiments that improves inference of gene fitness effects by Joshua M. Dempster, Isabella Boyle, Francisca Vazquez, David E. Root, Jesse S. Boehm, William C. Hahn, Aviad Tsherniak, James M. McFarland

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Aire-dependent genes undergo Clp1-mediated 3’UTR shortening associated with higher transcript stability in the thymus by Clotilde Guyon, Nada Jmari, Francine Padonou, Yen-Chin Li, Olga Ucar, Noriyuki Fujikado, Fanny Coulpier, Christophe Blanchet, David E Root, Matthieu Giraud

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Morphological Profiles of RNAi-Induced Gene Knockdown Are Highly Reproducible but Dominated by Seed Effects. by Shantanu Singh, Xiaoyun Wu, Vebjorn Ljosa, Mark-Anthony Bray, Federica Piccioni, David E Root, John G Doench, Jesse S Boehm, Anne E Carpenter

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High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons. by Thomas J F Nieland, David J Logan, Jessica Saulnier, Daniel Lam, Caroline Johnson, David E Root, Anne E Carpenter, Bernardo L Sabatini

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A Targeted RNA Interference Screen Reveals Novel Epigenetic Factors That Regulate Herpesviral Gene Expression by Hyung Suk Oh, Kevin F. Bryant, Thomas J. F. Nieland, Aprotim Mazumder, Mukta Bagul, Mark Bathe, David E. Root, David M. Knipe

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Targeted shRNA screening identified critical roles of pleckstrin-2 in erythropoiesis by Baobing Zhao, Ganesan Keerthivasan, Yang Mei, Jing Yang, James McElherne, Piu Wong, John G. Doench, Gang Feng, David E. Root, Peng Ji

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CRISPR-Cas9 screens reveal common essential miRNAs in human cancer cell lines by Daniel J. Merk, Linda Paul, Foteini Tsiami, Helen Hohenthanner, Ghazal Mohseni Kouchesfahani, Lara A. Haeusser, Bianca Walter, Adam Brown, Nicole S. Persky, David E. Root, Ghazaleh Tabatabai

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Evaluation of RNAi and CRISPR technologies by large-scale gene expression profiling in the Connectivity Map. by Ian Smith, Peyton G Greenside, Ted Natoli, David L Lahr, David Wadden, Itay Tirosh, Rajiv Narayan, David E Root, Todd R Golub, Aravind Subramanian, John G Doench

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Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities by Kendall R. Sanson, Ruth E. Hanna, Mudra Hegde, Katherine F. Donovan, Christine Strand, Meagan E. Sullender, Emma W. Vaimberg, Amy Goodale, David E. Root, Federica Piccioni, John G. Doench

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Genome-wide CRISPR-Cas9 knockout screens identify DNMT1 as a druggable dependency in sonic hedgehog medulloblastoma by Foteini Tsiami, Chiara Lago, Noemi Pozza, Federica Piccioni, Xuesong Zhao, Fabienne Lülsberg, David E. Root, Luca Tiberi, Marcel Kool, Jens Schittenhelm, Pratiti Bandopadhayay, Rosalind A. Segal, Ghazaleh Tabatabai, Daniel J. Merk

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Partial gene suppression improves identification of cancer vulnerabilities when CRISPR-Cas9 knockout is pan-lethal by J. Michael Krill-Burger, Joshua M. Dempster, Ashir A. Borah, Brenton R. Paolella, David E. Root, Todd R. Golub, Jesse S. Boehm, William C. Hahn, James M. McFarland, Francisca Vazquez, Aviad Tsherniak

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The master regulator of the cellular stress response (HSF1) is critical for orthopoxvirus infection. by Claire Marie Filone, Ignacio S Caballero, Ken Dower, Marc L Mendillo, Glenn S Cowley, Sandro Santagata, Daniel K Rozelle, Judy Yen, Kathleen H Rubins, Nir Hacohen, David E Root, Lisa E Hensley, John Connor

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Correction: Corrigendum: PARP3 is a promoter of chromosomal rearrangements and limits G4 DNA by Tovah A. Day, Jacob V. Layer, J. Patrick Cleary, Srijoy Guha, Kristen E. Stevenson, Trevor Tivey, Sunhee Kim, Anna C Schinzel, Francesca Izzo, John Doench, David E. Root, William C. Hahn, Brendan D. Price, David M. Weinstock

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PARP3 is a promoter of chromosomal rearrangements and limits G4 DNA by Tovah A. Day, Jacob V. Layer, J. Patrick Cleary, Srijoy Guha, Kristen E. Stevenson, Trevor Tivey, Sunhee Kim, Anna C. Schinzel, Francesca Izzo, John Doench, David E. Root, William C. Hahn, Brendan D. Price, David M. Weinstock

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Mapping the landscape of genetic dependencies in chordoma by Tanaz Sharifnia, Mathias J. Wawer, Amy Goodale, Yenarae Lee, Mariya Kazachkova, Joshua M. Dempster, Sandrine Muller, Joan Levy, Daniel M. Freed, Josh Sommer, Jérémie Kalfon, Francisca Vazquez, William C. Hahn, David E. Root, Paul A. Clemons, Stuart L. Schreiber

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Phenotypic Characterization of a Comprehensive Set of MAPK1/ERK2 Missense Mutants by Lisa Brenan, Aleksandr Andreev, Ofir Cohen, Sasha Pantel, Atanas Kamburov, Davide Cacchiarelli, Nicole S. Persky, Cong Zhu, Mukta Bagul, Eva M. Goetz, Alex B. Burgin, Levi A. Garraway, Gad Getz, Tarjei S. Mikkelsen, Federica Piccioni, David E. Root, Cory M. Johannessen

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Germline variation contributes to false negatives in CRISPR-based experiments with varying burden across ancestries by Sean A. Misek, Aaron Fultineer, Jeremie Kalfon, Javad Noorbakhsh, Isabella Boyle, Priyanka Roy, Joshua Dempster, Lia Petronio, Katherine Huang, Alham Saadat, Thomas Green, Adam Brown, John G. Doench, David E. Root, James M. McFarland, Rameen Beroukhim, Jesse S. Boehm

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Regulation of Cellular Heterogeneity and Rates of Symmetric and Asymmetric Divisions in Triple-Negative Breast Cancer by Roy Z. Granit, Hadas Masury, Reba Condiotti, Yaakov Fixler, Yael Gabai, Tzofia Glikman, Simona Dalin, Eitan Winter, Yuval Nevo, Einat Carmon, Tamar Sella, Amir Sonnenblick, Tamar Peretz, Ulrich Lehmann, Keren Paz, Federica Piccioni, Aviv Regev, David E. Root, Ittai Ben-Porath

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