Tutorial: Guidelines for Single-Cell RT-qPCR by Daniel Zucha, Mikael Kubista, Lukas Valihrach

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Small RNA-Sequencing: Approaches and Considerations for miRNA Analysis by Sarka Benesova, Mikael Kubista, Lukas Valihrach

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Corrigendum: Reactive astrogliosis in the era of single-cell transcriptomics by Zuzana Matusova, Zuzana Matusova, Elly M. Hol, Milos Pekny, Milos Pekny, Milos Pekny, Mikael Kubista, Mikael Kubista, Lukas Valihrach, Lukas Valihrach

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Reactive astrogliosis in the era of single-cell transcriptomics by Zuzana Matusova, Zuzana Matusova, Elly M. Hol, Milos Pekny, Milos Pekny, Milos Pekny, Mikael Kubista, Mikael Kubista, Lukas Valihrach, Lukas Valihrach

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Thermodynamic characterization of the dimerization equilibrium of newly synthesized polymethine cyanine dyes by MIKAEL KUBISTA, TODOR G. DELIGEORGIEV, FERESHTEH SHIRI, JAHAN B. GHASEMI

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Heterogeneity of astrocytes: from development to injury - single cell gene expression. by Vendula Rusnakova, Pavel Honsa, David Dzamba, Anders Ståhlberg, Mikael Kubista, Miroslava Anderova

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Recent advances in deciphering oligodendrocyte heterogeneity with single-cell transcriptomics by Lukas Valihrach, Lukas Valihrach, Zuzana Matusova, Zuzana Matusova, Daniel Zucha, Daniel Zucha, Ruslan Klassen, Ruslan Klassen, Sarka Benesova, Sarka Benesova, Pavel Abaffy, Mikael Kubista, Mikael Kubista, Miroslava Anderova

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Distinct expression/function of potassium and chloride channels contributes to the diverse volume regulation in cortical astrocytes of GFAP/EGFP mice. by Jana Benesova, Vendula Rusnakova, Pavel Honsa, Helena Pivonkova, David Dzamba, Mikael Kubista, Miroslava Anderova

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Plasticity response in the contralesional hemisphere after subtle neurotrauma: gene expression profiling after partial deafferentation of the hippocampus. by Daniel Andersson, Ulrika Wilhelmsson, Michael Nilsson, Mikael Kubista, Anders Ståhlberg, Marcela Pekna, Milos Pekny

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Detection of Abundant Non-Haematopoietic Circulating Cancer-Related Cells in Patients with Advanced Epithelial Ovarian Cancer by Juhi Kumar, Dimple Chudasama, Charlotte Roberts, Mikael Kubista, Robert Sjöback, Jayanta Chatterjee, Vladimir Anikin, Emmanouil Karteris, Marcia Hall

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NormQ: RNASeq normalization based on RT-qPCR derived size factors by Ravindra Naraine, Pavel Abaffy, Monika Sidova, Silvie Tomankova, Kseniia Pocherniaieva, Ondrej Smolik, Mikael Kubista, Martin Psenicka, Radek Sindelka

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Metformin Increases Proliferative Activity and Viability of Multipotent Stromal Stem Cells Isolated from Adipose Tissue Derived from Horses with Equine Metabolic Syndrome by Agnieszka Smieszek, Katarzyna Kornicka, Jolanta Szłapka-Kosarzewska, Peter Androvic, Lukas Valihrach, Lucie Langerova, Eva Rohlova, Mikael Kubista, Krzysztof Marycz

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Analysis of in Vitro and in Vivo Characteristics of Human Embryonic Stem Cell-Derived Neural Precursors by Nataliya Kozubenko, Karolina Turnovcova, Miroslava Kapcalova, Olena Butenko, Miroslava Anderova, Vendula Rusnakova, Mikael Kubista, Ales Hampl, Pavla Jendelova, Eva Sykova

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Altered astrocytic swelling in the cortex of α-syntrophin-negative GFAP/EGFP mice. by Miroslava Anderova, Jana Benesova, Michaela Mikesova, David Dzamba, Pavel Honsa, Jan Kriska, Olena Butenko, Vendula Novosadova, Lukas Valihrach, Mikael Kubista, Lesia Dmytrenko, Michal Cicanic, Lydia Vargova

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SPIDIA-RNA: second external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses. by Francesca Malentacchi, Mario Pazzagli, Lisa Simi, Claudio Orlando, Ralf Wyrich, Kalle Günther, Paolo Verderio, Sara Pizzamiglio, Chiara Maura Ciniselli, Hui Zhang, Vlasta Korenková, Lynne Rainen, Tzachi Bar, Mikael Kubista, Stefania Gelmini

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Biomarkers for monitoring pre-analytical quality variation of mRNA in blood samples. by Hui Zhang, Vlasta Korenková, Robert Sjöback, David Švec, Jens Björkman, Mogens Kruhøffer, Paolo Verderio, Sara Pizzamiglio, Chiara Maura Ciniselli, Ralf Wyrich, Uwe Oelmueller, Mikael Kubista, Torbjørn Lindahl, Anders Lönneborg, Edith Rian

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Multicenter Evaluation of Independent High-Throughput and RT-qPCR Technologies for the Development of Analytical Workflows for Circulating miRNA Analysis by Anna Babayan, Martin H. D. Neumann, Andrei Herdean, Jonathan M. Shaffer, Melanie Janning, Franca Kobus, Sonja Loges, Francesca Di Pasquale, Mikael Kubista, Martin Schlumpberger, Rita Lampignano, Thomas Krahn, Thomas Schlange, Markus Sprenger-Haussels, Klaus Pantel, Vera Kloten

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Complement C3a treatment accelerates recovery after stroke via modulation of astrocyte reactivity and cortical connectivity by Anna Stokowska, Markus Aswendt, Daniel Zucha, Stephanie Lohmann, Frederique Wieters, Javier Morán Suarez, Alison L. Atkins, YiXian Li, Maria Miteva, Julia Lewin, Dirk Wiedermann, Michael Diedenhofen, Åsa Torinsson Naluai, Pavel Abaffy, Lukas Valihrach, Mikael Kubista, Mathias Hoehn, Milos Pekny, Marcela Pekna

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Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients by Svenja Schneegans, Lelia Lück, Katharina Besler, Leonie Bluhm, Julia‐Christina Stadler, Janina Staub, Rüdiger Greinert, Beate Volkmer, Mikael Kubista, Christoffer Gebhardt, Alexander Sartori, Darryl Irwin, Elina Serkkola, Taija afHällström, Evi Lianidou, Markus Sprenger‐Haussels, Melanie Hussong, Peter Mohr, Stefan W. Schneider, Jonathan Shaffer, Klaus Pantel, Harriet Wikman

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Alternative assembly of respiratory complex II connects energy stress to metabolic checkpoints by Ayenachew Bezawork-Geleta, He Wen, LanFeng Dong, Bing Yan, Jelena Vider, Stepana Boukalova, Linda Krobova, Katerina Vanova, Renata Zobalova, Margarita Sobol, Pavel Hozak, Silvia Magalhaes Novais, Veronika Caisova, Pavel Abaffy, Ravindra Naraine, Ying Pang, Thiri Zaw, Ping Zhang, Radek Sindelka, Mikael Kubista, Steven Zuryn, Mark P. Molloy, Michael V. Berridge, Karel Pacak, Jakub Rohlena, Sunghyouk Park, Jiri Neuzil

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