Overview of a high-throughput pipeline for streamlining the production of recombinant proteins by Rada, H, Nettleship, JE, Owens, RJ

Optimization of small-scale production of Zika virus envelope glycoprotein by transient expression in HEK293 cells for ELISA by Kim, YC, Dumoux, M, Owens, RJ, Reyes-Sandoval, A

Schistosoma mansoni cathepsin D1: biochemical and biophysical characterization of the recombinant enzyme expressed in HEK293T cells by Araujo-Montoya, BO, Senger, MR, Gomes, BF, Harris, G, Owens, RJ, Silva-Jr, FP

Transient transfection and expression of eukaryotic membrane proteins in Expi293F cells and their screening on a small scale: application for structural studies by Krasnoselska, GO, Dumoux, M, Gamage, N, Cheruvara, H, Birch, J, Quigley, A, Owens, RJ

From llama to nanobody: a streamlined workflow for the generation of functionalised VHHs by Eyssen, LEA, Ramadurai, S, Abdelkarim, S, Buckle, I, Cornish, K, Lin, H, Jones, AK, Stephens, GJ, Owens, RJ

Structural basis of antifolate recognition and transport by PCFT by Parker, JL, Deme, JC, Kuteyi, G, Wu, Z, Huo, J, Goldman, ID, Owens, RJ, Biggin, PC, Lea, SM, Newstead, S

The impact of exchanging the light and heavy chains on the structures of bovine ultralong antibodies by Clarke, JD, Douangamath, A, Mikolajek, H, Bonnet-Di Placido, M, Ren, J, Fry, EE, Stuart, DI, Hammond, JA, Owens, RJ

SARS-CoV-2 triggers pericyte-mediated cerebral capillary constriction by Hirunpattarasilp, C, James, G, Kwanthongdee, J, Freitas, F, Huo, J, Sethi, H, Kittler, JT, Owens, RJ, McCoy, LE, Attwell, D

Characterization of a Schistosoma mansoni NDPK expressed in sexual and digestive organs by Torini, JR, De Freitas Fernandes, A, Serrão, VH, Romanello, L, Bird, LE, Nettleship, JE, Owens, RJ, Brandão-Neto, J, Zeraik, AE, Demarco, R, Pereira, H

In vitro and in vivo characterization of the multiple isoforms of Schistosoma mansoni hypoxanthine-guanine phosphoribosyltransferases by Romanello, L, Zeraik, AE, De Freitas Fernandes, A, Torini, JR, Bird, LE, Nettleship, JE, Rada, H, Reddivari, Y, Owens, RJ, Serrão, VHB, Demarco, R, Brandão-Neto, J, Pereira, HD

Affimer proteins inhibit immune complex binding to FcγRIIIa with high specificity through competitive and allosteric modes of action by Robinson, JI, Baxter, EW, Owen, RL, Thomsen, M, Tomlinson, DC, Waterhouse, MP, Win, SJ, Nettleship, JE, Tiede, C, Foster, RJ, Owens, RJ, Fishwick, CWG, Harris, SA, Goldman, A, McPherson, MJ, Morgan, AW

Extracellular modulation of TREK-2 activity with nanobodies provides insight into the mechanisms of K2P channel regulation by Rödström, KEJ, Cloake, A, Sörmann, J, Baronina, A, Smith, KHM, Pike, ACW, Ang, J, Proks, P, Schewe, M, Holland-Kaye, I, Bushell, SR, Elliott, J, Pardon, E, Baukrowitz, T, Owens, RJ, Newstead, S, Steyaert, J, Carpenter, EP, Tucker, SJ

Structural and functional characterization of nanobodies that neutralize Omicron variants of SARS-CoV-2 by Cornish, K, Huo, J, Jones, L, Sharma, P, Thrush, JW, Abdelkarim, S, Kipar, A, Ramadurai, S, Weckener, M, Mikolajek, H, Liu, S, Buckle, I, Bentley, E, Kirby, A, Han, X, Laidlaw, SM, Hill, M, Eyssen, L, Norman, C, Le Bas, A, Clarke, J, James, W, Stewart, JP, Carroll, M, Naismith, JH, Owens, RJ

GPC3-Unc5 receptor complex structure and role in cell migration by Akkermans, O, Delloye-Bourgeois, C, Peregrina, C, Carrasquero-Ordaz, M, Kokolaki, M, Berbeira-Santana, M, Chavent, M, Reynaud, F, Raj, R, Agirre, J, Aksu, M, White, ES, Lowe, E, Ben Amar, D, Zaballa, S, Huo, J, Pakos, I, McCubbin, PTN, Comoletti, D, Owens, RJ, Robinson, CV, Castellani, V, del Toro, D, Seiradake, E

Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens by Fogg, MJ, Alzari, P, Bahar, M, Bertini, I, Betton, JM, Burmeister, WP, Cambillau, C, Canard, B, Corrondo, MA, Coll, M, Daenke, S, Dym, O, Egloff, MP, Enguita, FJ, Geerlof, A, Haouz, A, Jones, TA, Ma, Q, Manicka, SN, Migliardi, M, Nordlund, P, Owens, RJ, Peleg, Y, Schneider, G, Schnell, R, Stuart, DI, Tarbouriech, N, Unge, T, Wilkinson, AJ, Wilmanns, M, Wilson, KS, Zimhony, O, Grimes, JM

Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2 by Huo, J, Le Bas, A, Ruza, RR, Duyvesten, HME, Mikolajek, H, Malinauskas, T, Tan, TK, Rijal, P, Dumoux, M, Ward, PN, Ren, J, Zhou, D, Harrison, PJ, Weckener, M, Clare, DK, Vogirala, VK, Radecke, J, Moynié, L, Zhao, Y, Gilbert-Jaramillo, J, Knight, ML, Tree, JA, Buttigieg, KR, Coombes, N, Elmore, MJ, Carroll, MW, Carrique, L, Shah, PNM, James, W, Townsend, AR, Stuart, DI, Owens, RJ, Naismith, JH

A potent SARS-CoV-2 neutralising nanobody shows therapeutic efficacy in the Syrian golden hamster model of COVID-19 by Huo, J, Mikolajek, H, Le Bas, A, Clark, JJ, Sharma, P, Kipar, A, Dormon, J, Norman, C, Weckener, M, Clare, DK, Harrison, PJ, Tree, JA, Buttigieg, KR, Salguero, FJ, Watson, R, Knott, D, Carnell, O, Ngabo, D, Elmore, MJ, Fotheringham, S, Harding, A, Moynié, L, Ward, PN, Dumoux, M, Prince, T, Hall, Y, Hiscox, JA, Owen, A, James, W, Carroll, MW, Stewart, JP, Naismith, JH, Owens, RJ

Pathogen-sugar interactions revealed by universal saturation transfer analysis by Buchanan, CJ, Gaunt, B, Harrison, PJ, Yang, Y, Liu, J, Khan, A, Giltrap, AM, Le Bas, A, Ward, PN, Gupta, K, Dumoux, M, Tan, TK, Schimaski, L, Daga, S, Picchiotti, N, Baldassarri, M, Benetti, E, Fallerini, C, Fava, F, Giliberti, A, Koukos, PI, Davy, MJ, Lakshminarayanan, A, Xue, X, Papadakis, G, Deimel, LP, Casablancas-Antràs, V, Claridge, TDW, Bonvin, AMJJ, Sattentau, QJ, Furini, S, Gori, M, Huo, J, Owens, RJ, Schaffitzel, C, Berger, I, Renieri, A, Naismith, JH, Baldwin, AJ, Davis, BG

Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient by Zhou, D, Duyvesteyn, HME, Chen, C-P, Huang, C-G, Chen, T-H, Shih, S-R, Lin, Y-C, Cheng, C-Y, Cheng, S-H, Huang, Y-C, Lin, T-Y, Ma, C, Huo, J, Carrique, L, Malinauskas, T, Ruza, RR, Shah, PNM, Tan, TK, Rijal, P, Donat, RF, Godwin, K, Buttigieg, KR, Tree, JA, Radecke, J, Paterson, NG, Supasa, P, Mongkolsapaya, J, Screaton, GR, Carroll, MW, Gilbert-Jaramillo, J, Knight, ML, James, W, Owens, RJ, Naismith, JH, Townsend, AR, Fry, EE, Zhao, Y, Ren, J, Stuart, DI, Huang, K-YA

Affimer proteins are versatile and renewable affinity reagents. by Tiede, C, Bedford, R, Heseltine, S, Smith, G, Wijetunga, I, Ross, R, AlQallaf, D, Roberts, APE, Balls, A, Curd, A, Hughes, RE, Martin, H, Needham, SR, Zanetti-Domingues, LC, Sadigh, Y, Peacock, TP, Tang, AA, Gibson, N, Kyle, H, Platt, GW, Ingram, N, Taylor, T, Coletta, LP, Manfield, I, Knowles, M, Bell, S, Esteves, F, Maqbool, A, Prasad, RK, Drinkhill, M, Bon, RS, Patel, V, Goodchild, SA, Martin-Fernandez, M, Owens, RJ, Nettleship, JE, Webb, ME, Harrison, M, Lippiat, JD, Ponnambalam, S, Peckham, M, Smith, A, Ferrigno, PK, Johnson, M, McPherson, MJ, Tomlinson, DC