A nove biological function of alginate in 'pseudomonasaeruginosa' and its mucoid mutants: stimulation of exolipase/
Treatment of 'Pseudomonas aeruginosa' ATCC9027 with various commercial alginates from brown algae enhanced extracellular lipase activities in time and concentration-dependent manner ("exolipase stimulation"). Alginate isolated from 'Azotobacter vinelandii' and mucoid mu...
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author | 355538 Wingender, Jost Winkler, Ulrich K. |
author_facet | 355538 Wingender, Jost Winkler, Ulrich K. |
author_sort | 355538 Wingender, Jost |
collection | OCEAN |
description | Treatment of 'Pseudomonas aeruginosa' ATCC9027 with various commercial alginates from brown algae enhanced extracellular lipase activities in time and concentration-dependent manner ("exolipase stimulation"). Alginate isolated from 'Azotobacter vinelandii' and mucoid mutants of 'P. aeruginosa' was similarly effective. Several independently isolated mucoid (alginate-producing) mutants of 'P. aeruginosa' showed higher spontaneous exolipase activities than the non-mucoid wild type. Alginate was chemically modified by (I) reduction of carboxyl groups (removal of charge), (II) oxidation of pyranoid rings (destruction of tertiary structure), and (III) reduction of reducing end groups. None of the chemical modifications resulted in total loss of the exolipase-stimulating ability of the alginate derivatives. |
first_indexed | 2024-03-05T07:50:40Z |
format | |
id | KOHA-OAI-TEST:386944 |
institution | Universiti Teknologi Malaysia - OCEAN |
last_indexed | 2024-03-05T07:50:40Z |
record_format | dspace |
spelling | KOHA-OAI-TEST:3869442020-12-19T17:13:30ZA nove biological function of alginate in 'pseudomonasaeruginosa' and its mucoid mutants: stimulation of exolipase/ 355538 Wingender, Jost Winkler, Ulrich K. Treatment of 'Pseudomonas aeruginosa' ATCC9027 with various commercial alginates from brown algae enhanced extracellular lipase activities in time and concentration-dependent manner ("exolipase stimulation"). Alginate isolated from 'Azotobacter vinelandii' and mucoid mutants of 'P. aeruginosa' was similarly effective. Several independently isolated mucoid (alginate-producing) mutants of 'P. aeruginosa' showed higher spontaneous exolipase activities than the non-mucoid wild type. Alginate was chemically modified by (I) reduction of carboxyl groups (removal of charge), (II) oxidation of pyranoid rings (destruction of tertiary structure), and (III) reduction of reducing end groups. None of the chemical modifications resulted in total loss of the exolipase-stimulating ability of the alginate derivatives.Treatment of 'Pseudomonas aeruginosa' ATCC9027 with various commercial alginates from brown algae enhanced extracellular lipase activities in time and concentration-dependent manner ("exolipase stimulation"). Alginate isolated from 'Azotobacter vinelandii' and mucoid mutants of 'P. aeruginosa' was similarly effective. Several independently isolated mucoid (alginate-producing) mutants of 'P. aeruginosa' showed higher spontaneous exolipase activities than the non-mucoid wild type. Alginate was chemically modified by (I) reduction of carboxyl groups (removal of charge), (II) oxidation of pyranoid rings (destruction of tertiary structure), and (III) reduction of reducing end groups. None of the chemical modifications resulted in total loss of the exolipase-stimulating ability of the alginate derivatives.39PSZJBLPseudomonas aeruginosaAlginates |
spellingShingle | Pseudomonas aeruginosa Alginates 355538 Wingender, Jost Winkler, Ulrich K. A nove biological function of alginate in 'pseudomonasaeruginosa' and its mucoid mutants: stimulation of exolipase/ |
title | A nove biological function of alginate in 'pseudomonasaeruginosa' and its mucoid mutants: stimulation of exolipase/ |
title_full | A nove biological function of alginate in 'pseudomonasaeruginosa' and its mucoid mutants: stimulation of exolipase/ |
title_fullStr | A nove biological function of alginate in 'pseudomonasaeruginosa' and its mucoid mutants: stimulation of exolipase/ |
title_full_unstemmed | A nove biological function of alginate in 'pseudomonasaeruginosa' and its mucoid mutants: stimulation of exolipase/ |
title_short | A nove biological function of alginate in 'pseudomonasaeruginosa' and its mucoid mutants: stimulation of exolipase/ |
title_sort | nove biological function of alginate in pseudomonasaeruginosa and its mucoid mutants stimulation of exolipase |
topic | Pseudomonas aeruginosa Alginates |
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