Biobutanol Production in a Mixed Culture System of Clostridium Acteobutylicum ATCC 824 and Bacillus Subtilis DSM 4451

In this study, the mix culture system consists of anaerobic Clostridium acetobutylicum and aerobic Bacillus subtilis was used to develop an anaerobic condition in the fermentation. B. subtilis consumed oxygen in the broth and maintaining anaerobic condition for C. acetobutylicum to grow. Hence, the...

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Main Authors: N. Nor, Azaini, N., Masngut
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/10581/1/Biobutanol%20production%20in%20a%20mixed%20culture%20system%20of%20Clostridium%20acteobutylicum%20ATCC%20824%20and%20Bacillus%20subtilis%20DSM%204451.pdf
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author N. Nor, Azaini
N., Masngut
author_facet N. Nor, Azaini
N., Masngut
author_sort N. Nor, Azaini
collection UMP
description In this study, the mix culture system consists of anaerobic Clostridium acetobutylicum and aerobic Bacillus subtilis was used to develop an anaerobic condition in the fermentation. B. subtilis consumed oxygen in the broth and maintaining anaerobic condition for C. acetobutylicum to grow. Hence, the culture was maintained in anaerobic condition without the use of N2 gas. Comparison between pure culture fermentation of C. acetobutylicum (control) with the mixed culture was carried out. The parameter studied was the inoculum ratio of C. acetobutylicum to the B. subtilis in the mixed culture fermentation which are 1:1, 1:2, 1:3, 2:1 and 3:1. The result shows that ratio of 1:3 mixed culture produced the highest butanol with 0.25 g/L which is almost twice than that in control fermentation. Hence, it shows that the butanol can be produced from mixed culture of C. acetobutylicum and B. subtilis.
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spelling UMPir105812018-01-15T01:49:24Z http://umpir.ump.edu.my/id/eprint/10581/ Biobutanol Production in a Mixed Culture System of Clostridium Acteobutylicum ATCC 824 and Bacillus Subtilis DSM 4451 N. Nor, Azaini N., Masngut TP Chemical technology In this study, the mix culture system consists of anaerobic Clostridium acetobutylicum and aerobic Bacillus subtilis was used to develop an anaerobic condition in the fermentation. B. subtilis consumed oxygen in the broth and maintaining anaerobic condition for C. acetobutylicum to grow. Hence, the culture was maintained in anaerobic condition without the use of N2 gas. Comparison between pure culture fermentation of C. acetobutylicum (control) with the mixed culture was carried out. The parameter studied was the inoculum ratio of C. acetobutylicum to the B. subtilis in the mixed culture fermentation which are 1:1, 1:2, 1:3, 2:1 and 3:1. The result shows that ratio of 1:3 mixed culture produced the highest butanol with 0.25 g/L which is almost twice than that in control fermentation. Hence, it shows that the butanol can be produced from mixed culture of C. acetobutylicum and B. subtilis. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/10581/1/Biobutanol%20production%20in%20a%20mixed%20culture%20system%20of%20Clostridium%20acteobutylicum%20ATCC%20824%20and%20Bacillus%20subtilis%20DSM%204451.pdf N. Nor, Azaini and N., Masngut (2015) Biobutanol Production in a Mixed Culture System of Clostridium Acteobutylicum ATCC 824 and Bacillus Subtilis DSM 4451. In: The International Conference on Fluids and Chemical Engineering (FLUIDSCHE 2015) , 25-27 Nov 2015 , Langkawi, Kedah. . (Unpublished)
spellingShingle TP Chemical technology
N. Nor, Azaini
N., Masngut
Biobutanol Production in a Mixed Culture System of Clostridium Acteobutylicum ATCC 824 and Bacillus Subtilis DSM 4451
title Biobutanol Production in a Mixed Culture System of Clostridium Acteobutylicum ATCC 824 and Bacillus Subtilis DSM 4451
title_full Biobutanol Production in a Mixed Culture System of Clostridium Acteobutylicum ATCC 824 and Bacillus Subtilis DSM 4451
title_fullStr Biobutanol Production in a Mixed Culture System of Clostridium Acteobutylicum ATCC 824 and Bacillus Subtilis DSM 4451
title_full_unstemmed Biobutanol Production in a Mixed Culture System of Clostridium Acteobutylicum ATCC 824 and Bacillus Subtilis DSM 4451
title_short Biobutanol Production in a Mixed Culture System of Clostridium Acteobutylicum ATCC 824 and Bacillus Subtilis DSM 4451
title_sort biobutanol production in a mixed culture system of clostridium acteobutylicum atcc 824 and bacillus subtilis dsm 4451
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/10581/1/Biobutanol%20production%20in%20a%20mixed%20culture%20system%20of%20Clostridium%20acteobutylicum%20ATCC%20824%20and%20Bacillus%20subtilis%20DSM%204451.pdf
work_keys_str_mv AT nnorazaini biobutanolproductioninamixedculturesystemofclostridiumacteobutylicumatcc824andbacillussubtilisdsm4451
AT nmasngut biobutanolproductioninamixedculturesystemofclostridiumacteobutylicumatcc824andbacillussubtilisdsm4451