Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix
In this study, enzymatic oxidation of glycerol was performed for the production of glyceric acid. The effectiveness of immobilised laccase in Na-Alginate matrix was also verified. Glycerol was incubated at 25oC with 30 mM of 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO), which acted as a mediator, in...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier Ltd
2015
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| Online Access: | http://umpir.ump.edu.my/id/eprint/11773/1/Enzymatic%20Conversion%20of%20Glycerol%20to%20Glyceric%20Acid%20with%20Immobilised%20Laccase%20in%20Na-Alginate%20Matrix.pdf |
| _version_ | 1796991033514393600 |
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| author | Chi, Shein Hong Chin, S. Y. Cheng, C. K. Mohd Sabri, Mahmud Chua, Gek Kee |
| author_facet | Chi, Shein Hong Chin, S. Y. Cheng, C. K. Mohd Sabri, Mahmud Chua, Gek Kee |
| author_sort | Chi, Shein Hong |
| collection | UMP |
| description | In this study, enzymatic oxidation of glycerol was performed for the production of glyceric acid. The effectiveness of immobilised laccase in Na-Alginate matrix was also verified. Glycerol was incubated at 25oC with 30 mM of 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO), which acted as a mediator, in the presence of immobilized laccase from Trametes versicolor. Glyceric acid was quantified using HPLC, while the activity of enzyme was measured using ABTS assay. The yield of laccase activity was 90%. On the other hand, glyceric acid obtained was around 7 wt% from the total product at 24 h. In conclusion, enzymatic oxidation is able to produce comparable amount of glyceric acid through a more environmental friendly method. |
| first_indexed | 2024-03-06T12:00:19Z |
| format | Article |
| id | UMPir11773 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:00:19Z |
| publishDate | 2015 |
| publisher | Elsevier Ltd |
| record_format | dspace |
| spelling | UMPir117732018-01-11T02:39:39Z http://umpir.ump.edu.my/id/eprint/11773/ Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix Chi, Shein Hong Chin, S. Y. Cheng, C. K. Mohd Sabri, Mahmud Chua, Gek Kee TP Chemical technology In this study, enzymatic oxidation of glycerol was performed for the production of glyceric acid. The effectiveness of immobilised laccase in Na-Alginate matrix was also verified. Glycerol was incubated at 25oC with 30 mM of 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO), which acted as a mediator, in the presence of immobilized laccase from Trametes versicolor. Glyceric acid was quantified using HPLC, while the activity of enzyme was measured using ABTS assay. The yield of laccase activity was 90%. On the other hand, glyceric acid obtained was around 7 wt% from the total product at 24 h. In conclusion, enzymatic oxidation is able to produce comparable amount of glyceric acid through a more environmental friendly method. Elsevier Ltd 2015 Article PeerReviewed application/pdf en cc_by_nc_nd http://umpir.ump.edu.my/id/eprint/11773/1/Enzymatic%20Conversion%20of%20Glycerol%20to%20Glyceric%20Acid%20with%20Immobilised%20Laccase%20in%20Na-Alginate%20Matrix.pdf Chi, Shein Hong and Chin, S. Y. and Cheng, C. K. and Mohd Sabri, Mahmud and Chua, Gek Kee (2015) Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix. Procedia Chemistry, 16. pp. 632-639. ISSN 1876-6196. (Published) http://dx.doi.org/10.1016/j.proche.2015.12.102 DOI: 10.1016/j.proche.2015.12.102 |
| spellingShingle | TP Chemical technology Chi, Shein Hong Chin, S. Y. Cheng, C. K. Mohd Sabri, Mahmud Chua, Gek Kee Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix |
| title | Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix |
| title_full | Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix |
| title_fullStr | Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix |
| title_full_unstemmed | Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix |
| title_short | Enzymatic Conversion of Glycerol to Glyceric Acid with Immobilised Laccase in Na-Alginate Matrix |
| title_sort | enzymatic conversion of glycerol to glyceric acid with immobilised laccase in na alginate matrix |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/11773/1/Enzymatic%20Conversion%20of%20Glycerol%20to%20Glyceric%20Acid%20with%20Immobilised%20Laccase%20in%20Na-Alginate%20Matrix.pdf |
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