Kinetic Study of Enzymatic Oxidation On Glycerol with Immobilised Laccase/TEMPO System
Glycerol, a byproduct of biodiesel has a great potential in producing valuable fine chemicals. Current catalytic oxidation methods based on noble metal have their drawbacks on environment. Towards this end, an enzymatic oxidation of glycerol by laccase from Trametes Versicolor and TEMPO (2,2,6,6-tet...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/12420/1/Kinetic%20Study%20of%20Enzymatic%20Oxidation0001.pdf |
| _version_ | 1825822805953871872 |
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| author | Chi, Shein Hong Chin, S. Y. Chin, Fui Cheng Mohd Sabri, Mahmud Chua, Gek Kee |
| author_facet | Chi, Shein Hong Chin, S. Y. Chin, Fui Cheng Mohd Sabri, Mahmud Chua, Gek Kee |
| author_sort | Chi, Shein Hong |
| collection | UMP |
| description | Glycerol, a byproduct of biodiesel has a great potential in producing valuable fine chemicals. Current catalytic oxidation methods based on noble metal have their drawbacks on environment. Towards this end, an enzymatic oxidation of glycerol by laccase from Trametes Versicolor and TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl)to produce glyceric acid seems to be a promising approach. Immobilisation of enzyme increased laccase stability and reusability. Glycerol as initial reactant for the oxidation process selectively oxygenates by immobilised laccase/TEMPO leads to a complex system with different reaction rate for each component. Glycerol was incubated with 30mM of TEMPO with the presence of immobilised laccase for 24hours. Products were quantified by using HPLC.A kinetic model of the glycerol oxidation was developed. The whole reaction was divided into 2 steps. Experimental data calculated results for each step were obtained with good agreement. From the data, the kinetic for all the components was identified as well. These results can be used in developing new greener catalyst with high activity, selectivity and stability for glyceric acid. |
| first_indexed | 2024-03-06T12:02:03Z |
| format | Conference or Workshop Item |
| id | UMPir12420 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:02:03Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | UMPir124202018-02-06T00:55:00Z http://umpir.ump.edu.my/id/eprint/12420/ Kinetic Study of Enzymatic Oxidation On Glycerol with Immobilised Laccase/TEMPO System Chi, Shein Hong Chin, S. Y. Chin, Fui Cheng Mohd Sabri, Mahmud Chua, Gek Kee TP Chemical technology Glycerol, a byproduct of biodiesel has a great potential in producing valuable fine chemicals. Current catalytic oxidation methods based on noble metal have their drawbacks on environment. Towards this end, an enzymatic oxidation of glycerol by laccase from Trametes Versicolor and TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl)to produce glyceric acid seems to be a promising approach. Immobilisation of enzyme increased laccase stability and reusability. Glycerol as initial reactant for the oxidation process selectively oxygenates by immobilised laccase/TEMPO leads to a complex system with different reaction rate for each component. Glycerol was incubated with 30mM of TEMPO with the presence of immobilised laccase for 24hours. Products were quantified by using HPLC.A kinetic model of the glycerol oxidation was developed. The whole reaction was divided into 2 steps. Experimental data calculated results for each step were obtained with good agreement. From the data, the kinetic for all the components was identified as well. These results can be used in developing new greener catalyst with high activity, selectivity and stability for glyceric acid. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12420/1/Kinetic%20Study%20of%20Enzymatic%20Oxidation0001.pdf Chi, Shein Hong and Chin, S. Y. and Chin, Fui Cheng and Mohd Sabri, Mahmud and Chua, Gek Kee (2015) Kinetic Study of Enzymatic Oxidation On Glycerol with Immobilised Laccase/TEMPO System. In: Asian Congress on Biotechnology 2015 (ACB2015) , 15-19 November 2015 , Kuala Lumpur. . (Published) |
| spellingShingle | TP Chemical technology Chi, Shein Hong Chin, S. Y. Chin, Fui Cheng Mohd Sabri, Mahmud Chua, Gek Kee Kinetic Study of Enzymatic Oxidation On Glycerol with Immobilised Laccase/TEMPO System |
| title | Kinetic Study of Enzymatic Oxidation On Glycerol with Immobilised Laccase/TEMPO System |
| title_full | Kinetic Study of Enzymatic Oxidation On Glycerol with Immobilised Laccase/TEMPO System |
| title_fullStr | Kinetic Study of Enzymatic Oxidation On Glycerol with Immobilised Laccase/TEMPO System |
| title_full_unstemmed | Kinetic Study of Enzymatic Oxidation On Glycerol with Immobilised Laccase/TEMPO System |
| title_short | Kinetic Study of Enzymatic Oxidation On Glycerol with Immobilised Laccase/TEMPO System |
| title_sort | kinetic study of enzymatic oxidation on glycerol with immobilised laccase tempo system |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/12420/1/Kinetic%20Study%20of%20Enzymatic%20Oxidation0001.pdf |
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