Production and optimization of l-asparaginase by e.coli atcc10536 from food waste
The current study aimed at utilization of food waste as squid pen (SP) and cooked chicken bone (CCB) for Lasparaginase (LA) production and optimization using E.coli culture. During our investigations, high levels of Lasparaginase were obtained from E.coli using squid pen as a sole carbon source as c...
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| Format: | Article |
| Language: | English English |
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JOCPR
2015
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| Online Access: | http://umpir.ump.edu.my/id/eprint/12759/1/JCPR-2015-7-10-394-401.pdf http://umpir.ump.edu.my/id/eprint/12759/7/fist-2015-essam-production%20and%20optimization.pdf |
| _version_ | 1825822861284081664 |
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| author | Batool, Tahira Chia, Vi Vien Makky, Essam A. Nina Suhaity, Azmi Mashitah, Mohd Yusoff |
| author_facet | Batool, Tahira Chia, Vi Vien Makky, Essam A. Nina Suhaity, Azmi Mashitah, Mohd Yusoff |
| author_sort | Batool, Tahira |
| collection | UMP |
| description | The current study aimed at utilization of food waste as squid pen (SP) and cooked chicken bone (CCB) for Lasparaginase (LA) production and optimization using E.coli culture. During our investigations, high levels of Lasparaginase were obtained from E.coli using squid pen as a sole carbon source as compared to CCB. Optimization of parameters resulted in 2.5folds increase in the L-asparaginase activity. Optimum L-asparaginase production 4.316IU/mL was observed when production media was supplemented with 1%, (w/v) ammonium sulphate, 2%, (w/v) substrate at pH 9, and 37°C after incubation for 5 days.This study underlines the use of food waste as an alternate substrate for the production of L-asparaginase and to improve the cost effectiveness of this enzyme. |
| first_indexed | 2024-03-06T12:02:50Z |
| format | Article |
| id | UMPir12759 |
| institution | Universiti Malaysia Pahang |
| language | English English |
| last_indexed | 2024-03-06T12:02:50Z |
| publishDate | 2015 |
| publisher | JOCPR |
| record_format | dspace |
| spelling | UMPir127592023-04-03T07:22:59Z http://umpir.ump.edu.my/id/eprint/12759/ Production and optimization of l-asparaginase by e.coli atcc10536 from food waste Batool, Tahira Chia, Vi Vien Makky, Essam A. Nina Suhaity, Azmi Mashitah, Mohd Yusoff Q Science (General) The current study aimed at utilization of food waste as squid pen (SP) and cooked chicken bone (CCB) for Lasparaginase (LA) production and optimization using E.coli culture. During our investigations, high levels of Lasparaginase were obtained from E.coli using squid pen as a sole carbon source as compared to CCB. Optimization of parameters resulted in 2.5folds increase in the L-asparaginase activity. Optimum L-asparaginase production 4.316IU/mL was observed when production media was supplemented with 1%, (w/v) ammonium sulphate, 2%, (w/v) substrate at pH 9, and 37°C after incubation for 5 days.This study underlines the use of food waste as an alternate substrate for the production of L-asparaginase and to improve the cost effectiveness of this enzyme. JOCPR 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12759/1/JCPR-2015-7-10-394-401.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/12759/7/fist-2015-essam-production%20and%20optimization.pdf Batool, Tahira and Chia, Vi Vien and Makky, Essam A. and Nina Suhaity, Azmi and Mashitah, Mohd Yusoff (2015) Production and optimization of l-asparaginase by e.coli atcc10536 from food waste. Journal of Chemical and Pharmaceutical Research, 7 (10). pp. 394-401. ISSN 0975-7384. (Published) http://jocpr.com/vol7-iss10-2015/JCPR-2015-7-10-394-401.pdf |
| spellingShingle | Q Science (General) Batool, Tahira Chia, Vi Vien Makky, Essam A. Nina Suhaity, Azmi Mashitah, Mohd Yusoff Production and optimization of l-asparaginase by e.coli atcc10536 from food waste |
| title | Production and optimization of l-asparaginase by e.coli atcc10536 from food waste |
| title_full | Production and optimization of l-asparaginase by e.coli atcc10536 from food waste |
| title_fullStr | Production and optimization of l-asparaginase by e.coli atcc10536 from food waste |
| title_full_unstemmed | Production and optimization of l-asparaginase by e.coli atcc10536 from food waste |
| title_short | Production and optimization of l-asparaginase by e.coli atcc10536 from food waste |
| title_sort | production and optimization of l asparaginase by e coli atcc10536 from food waste |
| topic | Q Science (General) |
| url | http://umpir.ump.edu.my/id/eprint/12759/1/JCPR-2015-7-10-394-401.pdf http://umpir.ump.edu.my/id/eprint/12759/7/fist-2015-essam-production%20and%20optimization.pdf |
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