Microbiological Study against Bacterial Isolates from Different Patients

Tooth decay is considered the most widespread infectious disease in the world. This study aims to isolate and identify the important bacteria related to tooth decay, determine the sensitivity of bacteria in certain types of antimicrobial agents, and study the effect of heavy metals and virulence fa...

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Main Authors: Ali, Muna Jalal, Makky, Essam A., M. M., Yusoff
Format: Article
Language:English
Published: AENSI Publishing 2016
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/13544/1/fist-2016-essam-microbiology.pdf
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author Ali, Muna Jalal
Makky, Essam A.
M. M., Yusoff
author_facet Ali, Muna Jalal
Makky, Essam A.
M. M., Yusoff
author_sort Ali, Muna Jalal
collection UMP
description Tooth decay is considered the most widespread infectious disease in the world. This study aims to isolate and identify the important bacteria related to tooth decay, determine the sensitivity of bacteria in certain types of antimicrobial agents, and study the effect of heavy metals and virulence factors on oral bacterial isolates. A total of 50 swabs were collected from the mouths of patients from both gender, with ages ranging from 1–60 years. The patients were advised to consult with dental clinics and specialized centers to isolate and identify the causative agents associated with oral diseases. Results showed that infection rates in younger age groups (1–20 and 20–40) are higher than the elder group (40–60), with percent incidence of 44% and 32%, respectively. Antibiotic sensitivity test against the isolates showed that chloramphenicol had the highest sensitivity effect with 83.2% followed by rifampicin and gentamicin with 81.35%, penicillin G with 64.40%, and streptomycin with 16.94% Also, these differences were found have lower effect for isolates against (10) heavy metals, where it showed resistance to Iron 3.38%, then nickel, aluminum, copper, lead to 20.33%, 22.03%, 27.11%, 28.81% respectively, also sliver shown 57.62%. And, this similarity were found have sensitive to antimony and chromium 61.01%.while appeared sensitivity to mercury and cadmium by 100, 86.44. Hemolysin had the highest ability to produce virulence factors (72.88%), followed by lecithinase (42.37%) and protease (25.42%). Lipase and urease had the lowest virulence factor production (10.16%).
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spelling UMPir135442018-07-26T03:26:15Z http://umpir.ump.edu.my/id/eprint/13544/ Microbiological Study against Bacterial Isolates from Different Patients Ali, Muna Jalal Makky, Essam A. M. M., Yusoff QR Microbiology Tooth decay is considered the most widespread infectious disease in the world. This study aims to isolate and identify the important bacteria related to tooth decay, determine the sensitivity of bacteria in certain types of antimicrobial agents, and study the effect of heavy metals and virulence factors on oral bacterial isolates. A total of 50 swabs were collected from the mouths of patients from both gender, with ages ranging from 1–60 years. The patients were advised to consult with dental clinics and specialized centers to isolate and identify the causative agents associated with oral diseases. Results showed that infection rates in younger age groups (1–20 and 20–40) are higher than the elder group (40–60), with percent incidence of 44% and 32%, respectively. Antibiotic sensitivity test against the isolates showed that chloramphenicol had the highest sensitivity effect with 83.2% followed by rifampicin and gentamicin with 81.35%, penicillin G with 64.40%, and streptomycin with 16.94% Also, these differences were found have lower effect for isolates against (10) heavy metals, where it showed resistance to Iron 3.38%, then nickel, aluminum, copper, lead to 20.33%, 22.03%, 27.11%, 28.81% respectively, also sliver shown 57.62%. And, this similarity were found have sensitive to antimony and chromium 61.01%.while appeared sensitivity to mercury and cadmium by 100, 86.44. Hemolysin had the highest ability to produce virulence factors (72.88%), followed by lecithinase (42.37%) and protease (25.42%). Lipase and urease had the lowest virulence factor production (10.16%). AENSI Publishing 2016 Article PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/13544/1/fist-2016-essam-microbiology.pdf Ali, Muna Jalal and Makky, Essam A. and M. M., Yusoff (2016) Microbiological Study against Bacterial Isolates from Different Patients. Australian Journal of Basic and Applied Sciences, 10 (14). pp. 366-373. ISSN 1991-8178. (Published) http://www.ajbasweb.com/old/ajbas/2016/September/366-373.pdf
spellingShingle QR Microbiology
Ali, Muna Jalal
Makky, Essam A.
M. M., Yusoff
Microbiological Study against Bacterial Isolates from Different Patients
title Microbiological Study against Bacterial Isolates from Different Patients
title_full Microbiological Study against Bacterial Isolates from Different Patients
title_fullStr Microbiological Study against Bacterial Isolates from Different Patients
title_full_unstemmed Microbiological Study against Bacterial Isolates from Different Patients
title_short Microbiological Study against Bacterial Isolates from Different Patients
title_sort microbiological study against bacterial isolates from different patients
topic QR Microbiology
url http://umpir.ump.edu.my/id/eprint/13544/1/fist-2016-essam-microbiology.pdf
work_keys_str_mv AT alimunajalal microbiologicalstudyagainstbacterialisolatesfromdifferentpatients
AT makkyessama microbiologicalstudyagainstbacterialisolatesfromdifferentpatients
AT mmyusoff microbiologicalstudyagainstbacterialisolatesfromdifferentpatients