Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells
Flavokawain B (FKB) is known to possess promising anticancer abilities. This is demonstrated in various cancer cell lines including HeLa cells. Cervical cancer is among the most widely diagnosed cancer among women today. Though FKB has been shown to be effective in treating cancer cells, the exact m...
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| Format: | Article |
| Language: | English |
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SAGE Publications
2017
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| Online Access: | http://umpir.ump.edu.my/id/eprint/16811/1/fist-2016-nadeem-Gene%20Expression%20Analysis%20Reveals%20the3.pdf |
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| author | Swee, Keong Yeap Nadiah, Abu Akhtar, Muhammad Nadeem Wan, Yong Ho Huynh, Ky Sheau, Wei Tan Noorjahan Banu, Alitheen Tunku, Kamarul |
| author_facet | Swee, Keong Yeap Nadiah, Abu Akhtar, Muhammad Nadeem Wan, Yong Ho Huynh, Ky Sheau, Wei Tan Noorjahan Banu, Alitheen Tunku, Kamarul |
| author_sort | Swee, Keong Yeap |
| collection | UMP |
| description | Flavokawain B (FKB) is known to possess promising anticancer abilities. This is demonstrated in various cancer cell lines including HeLa cells. Cervical cancer is among the most widely diagnosed cancer among women today. Though FKB has been shown to be effective in treating cancer cells, the exact molecular mechanism is still unknown. This study is aimed at understanding the effects of FKB on HeLa cells using a microarray-based mRNA expression profiling and proteome profiling of stress-related proteins. The results of this study suggest that FKB induced cell death through p21-mediated cell cycle arrest and activation of p38. However, concurrent activation of antioxidant-related pathways and iron sequestration pathway followed by activation of ER-resident stress proteins clearly indicate that FKB failed to induce apoptosis in HeLa cells via oxidative stress. This effect implies that the protection of HeLa cells by FKB from H2O2–induced cell death is via neutralization of reactive oxygen species. |
| first_indexed | 2024-03-06T12:13:17Z |
| format | Article |
| id | UMPir16811 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:13:17Z |
| publishDate | 2017 |
| publisher | SAGE Publications |
| record_format | dspace |
| spelling | UMPir168112017-09-13T06:12:55Z http://umpir.ump.edu.my/id/eprint/16811/ Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells Swee, Keong Yeap Nadiah, Abu Akhtar, Muhammad Nadeem Wan, Yong Ho Huynh, Ky Sheau, Wei Tan Noorjahan Banu, Alitheen Tunku, Kamarul QD Chemistry Flavokawain B (FKB) is known to possess promising anticancer abilities. This is demonstrated in various cancer cell lines including HeLa cells. Cervical cancer is among the most widely diagnosed cancer among women today. Though FKB has been shown to be effective in treating cancer cells, the exact molecular mechanism is still unknown. This study is aimed at understanding the effects of FKB on HeLa cells using a microarray-based mRNA expression profiling and proteome profiling of stress-related proteins. The results of this study suggest that FKB induced cell death through p21-mediated cell cycle arrest and activation of p38. However, concurrent activation of antioxidant-related pathways and iron sequestration pathway followed by activation of ER-resident stress proteins clearly indicate that FKB failed to induce apoptosis in HeLa cells via oxidative stress. This effect implies that the protection of HeLa cells by FKB from H2O2–induced cell death is via neutralization of reactive oxygen species. SAGE Publications 2017 Article PeerReviewed application/pdf en cc_by_nc http://umpir.ump.edu.my/id/eprint/16811/1/fist-2016-nadeem-Gene%20Expression%20Analysis%20Reveals%20the3.pdf Swee, Keong Yeap and Nadiah, Abu and Akhtar, Muhammad Nadeem and Wan, Yong Ho and Huynh, Ky and Sheau, Wei Tan and Noorjahan Banu, Alitheen and Tunku, Kamarul (2017) Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells. Integrative Cancer Therapies, 16 (3). pp. 373-384. ISSN 1534-7354 (print); 1552-695X (online). (Published) http://dx.doi.org/10.1177/1534735416660383 DOI: 10.1177/1534735416660383 |
| spellingShingle | QD Chemistry Swee, Keong Yeap Nadiah, Abu Akhtar, Muhammad Nadeem Wan, Yong Ho Huynh, Ky Sheau, Wei Tan Noorjahan Banu, Alitheen Tunku, Kamarul Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells |
| title | Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells |
| title_full | Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells |
| title_fullStr | Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells |
| title_full_unstemmed | Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells |
| title_short | Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells |
| title_sort | gene expression analysis reveals the concurrent activation of proapoptotic and antioxidant defensive mechanisms in flavokawain b treated cervical cancer hela cells |
| topic | QD Chemistry |
| url | http://umpir.ump.edu.my/id/eprint/16811/1/fist-2016-nadeem-Gene%20Expression%20Analysis%20Reveals%20the3.pdf |
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