In Vitro Anticancer Activity of Au, Ag Nanoparticles Synthesized Using Commelina Nudiflora L.Aqueous Extract Against HCT-116 Colon Cancer Cells
Recently, metal nanoparticles have been getting great medical and social interests due to their potential physico-chemical properties such as higher affinity, low molecular weight, and larger surface area. The biosynthesized gold and silver nanoparticles are spherical, triangular in shape with an av...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2016
|
| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/16849/1/In%20Vitro%20Anticancer%20Activity.pdf |
| _version_ | 1825823583958466560 |
|---|---|
| author | Kuppusamy, Palaniselvam Ichwan, Solachuddin J. A. Putri Nur Hidayah, Al-Zikri Wastuti Hidayati, Suriyah Soundharrajan, Ilavenil Natanamurugaraj, Govindan Gaanty Pragas, Maniam M. M., Yusoff |
| author_facet | Kuppusamy, Palaniselvam Ichwan, Solachuddin J. A. Putri Nur Hidayah, Al-Zikri Wastuti Hidayati, Suriyah Soundharrajan, Ilavenil Natanamurugaraj, Govindan Gaanty Pragas, Maniam M. M., Yusoff |
| author_sort | Kuppusamy, Palaniselvam |
| collection | UMP |
| description | Recently, metal nanoparticles have been getting great medical and social interests due to their potential physico-chemical properties such as higher affinity, low molecular weight, and larger surface area. The biosynthesized gold and silver nanoparticles are spherical, triangular in shape with an average size of 24–150 nm as reported in our earlier studies. The biological properties of synthesized gold and silver nanoparticles are demonstrated in this paper. The different in vitro assays such as MTT, flow cytometry, and reverse transcription polymerase chain reaction (RTqPCR) techniques were used to evaluate the in vitro anticancer properties of synthesized metal nanoparticles. The biosynthesized gold and silver nanoparticles have shown reduced cell viability and increased cytotoxicity in HCT116 colon cancer cells with IC50 concentration of 200 and 100 μg/ml, respectively. The flow cytometry experiments revealed that the IC50 concentrations of gold and silver nanoparticle-treated cells that have significant changes were observed in the sub-G1 cell cycle phase compared with the positive control. Additionally, the relative messenger RNA (mRNA) gene expressions of HCT-116 cells were studied by RT-qPCR techniques. The pro-apoptotic genes such as PUMA (++), Caspase-3 (+), Caspase-8 (++), and Caspase9 (++) were upregulated in the treated HCT-116 cells compared with cisplatin. Overall, these findings have proved that the synthesized gold and silver nanoparticles could be potent anti-colon cancer drugs. |
| first_indexed | 2024-03-06T12:13:22Z |
| format | Article |
| id | UMPir16849 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:13:22Z |
| publishDate | 2016 |
| publisher | Springer |
| record_format | dspace |
| spelling | UMPir168492018-07-27T01:31:22Z http://umpir.ump.edu.my/id/eprint/16849/ In Vitro Anticancer Activity of Au, Ag Nanoparticles Synthesized Using Commelina Nudiflora L.Aqueous Extract Against HCT-116 Colon Cancer Cells Kuppusamy, Palaniselvam Ichwan, Solachuddin J. A. Putri Nur Hidayah, Al-Zikri Wastuti Hidayati, Suriyah Soundharrajan, Ilavenil Natanamurugaraj, Govindan Gaanty Pragas, Maniam M. M., Yusoff QK Botany Recently, metal nanoparticles have been getting great medical and social interests due to their potential physico-chemical properties such as higher affinity, low molecular weight, and larger surface area. The biosynthesized gold and silver nanoparticles are spherical, triangular in shape with an average size of 24–150 nm as reported in our earlier studies. The biological properties of synthesized gold and silver nanoparticles are demonstrated in this paper. The different in vitro assays such as MTT, flow cytometry, and reverse transcription polymerase chain reaction (RTqPCR) techniques were used to evaluate the in vitro anticancer properties of synthesized metal nanoparticles. The biosynthesized gold and silver nanoparticles have shown reduced cell viability and increased cytotoxicity in HCT116 colon cancer cells with IC50 concentration of 200 and 100 μg/ml, respectively. The flow cytometry experiments revealed that the IC50 concentrations of gold and silver nanoparticle-treated cells that have significant changes were observed in the sub-G1 cell cycle phase compared with the positive control. Additionally, the relative messenger RNA (mRNA) gene expressions of HCT-116 cells were studied by RT-qPCR techniques. The pro-apoptotic genes such as PUMA (++), Caspase-3 (+), Caspase-8 (++), and Caspase9 (++) were upregulated in the treated HCT-116 cells compared with cisplatin. Overall, these findings have proved that the synthesized gold and silver nanoparticles could be potent anti-colon cancer drugs. Springer 2016-10-09 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/16849/1/In%20Vitro%20Anticancer%20Activity.pdf Kuppusamy, Palaniselvam and Ichwan, Solachuddin J. A. and Putri Nur Hidayah, Al-Zikri and Wastuti Hidayati, Suriyah and Soundharrajan, Ilavenil and Natanamurugaraj, Govindan and Gaanty Pragas, Maniam and M. M., Yusoff (2016) In Vitro Anticancer Activity of Au, Ag Nanoparticles Synthesized Using Commelina Nudiflora L.Aqueous Extract Against HCT-116 Colon Cancer Cells. Biological Trace Element Research , 173 (2). pp. 297-305. ISSN 0163-4984(Print); 1559-0720(Online). (Published) https://dx.doi.org/10.1007/s12011-016-0666-7 DOI: 10.1007/s12011-016-0666-7 |
| spellingShingle | QK Botany Kuppusamy, Palaniselvam Ichwan, Solachuddin J. A. Putri Nur Hidayah, Al-Zikri Wastuti Hidayati, Suriyah Soundharrajan, Ilavenil Natanamurugaraj, Govindan Gaanty Pragas, Maniam M. M., Yusoff In Vitro Anticancer Activity of Au, Ag Nanoparticles Synthesized Using Commelina Nudiflora L.Aqueous Extract Against HCT-116 Colon Cancer Cells |
| title | In Vitro Anticancer Activity of Au, Ag Nanoparticles Synthesized Using Commelina Nudiflora L.Aqueous Extract Against HCT-116 Colon Cancer Cells
|
| title_full | In Vitro Anticancer Activity of Au, Ag Nanoparticles Synthesized Using Commelina Nudiflora L.Aqueous Extract Against HCT-116 Colon Cancer Cells
|
| title_fullStr | In Vitro Anticancer Activity of Au, Ag Nanoparticles Synthesized Using Commelina Nudiflora L.Aqueous Extract Against HCT-116 Colon Cancer Cells
|
| title_full_unstemmed | In Vitro Anticancer Activity of Au, Ag Nanoparticles Synthesized Using Commelina Nudiflora L.Aqueous Extract Against HCT-116 Colon Cancer Cells
|
| title_short | In Vitro Anticancer Activity of Au, Ag Nanoparticles Synthesized Using Commelina Nudiflora L.Aqueous Extract Against HCT-116 Colon Cancer Cells
|
| title_sort | in vitro anticancer activity of au ag nanoparticles synthesized using commelina nudiflora l aqueous extract against hct 116 colon cancer cells |
| topic | QK Botany |
| url | http://umpir.ump.edu.my/id/eprint/16849/1/In%20Vitro%20Anticancer%20Activity.pdf |
| work_keys_str_mv | AT kuppusamypalaniselvam invitroanticanceractivityofauagnanoparticlessynthesizedusingcommelinanudifloralaqueousextractagainsthct116coloncancercells AT ichwansolachuddinja invitroanticanceractivityofauagnanoparticlessynthesizedusingcommelinanudifloralaqueousextractagainsthct116coloncancercells AT putrinurhidayahalzikri invitroanticanceractivityofauagnanoparticlessynthesizedusingcommelinanudifloralaqueousextractagainsthct116coloncancercells AT wastutihidayatisuriyah invitroanticanceractivityofauagnanoparticlessynthesizedusingcommelinanudifloralaqueousextractagainsthct116coloncancercells AT soundharrajanilavenil invitroanticanceractivityofauagnanoparticlessynthesizedusingcommelinanudifloralaqueousextractagainsthct116coloncancercells AT natanamurugarajgovindan invitroanticanceractivityofauagnanoparticlessynthesizedusingcommelinanudifloralaqueousextractagainsthct116coloncancercells AT gaantypragasmaniam invitroanticanceractivityofauagnanoparticlessynthesizedusingcommelinanudifloralaqueousextractagainsthct116coloncancercells AT mmyusoff invitroanticanceractivityofauagnanoparticlessynthesizedusingcommelinanudifloralaqueousextractagainsthct116coloncancercells |