Fabrication, Characterization and In Vitro Biocompatibility of Electrospun Hydroxyethyl Cellulose/poly (vinyl) Alcohol Nanofibrous Composite Biomaterial for Bone Tissue Engineering

Development ofnovel scaffold materials that mimic the extracellular matrix, architecturally and func- tionally, is becoming highly important to meet the demands of the advances in bone tissue engineering. This paper reports, the fabrication of natural polymer cellulose derived hydroxyethyl cellulose...

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Main Authors: Chahal, Sugandha, Jahir Hussain, Fathima Shahitha, Kumar, Anuj, Mohammad Syaiful Bahari, Abdull Rasad, M. M., Yusoff
Format: Article
Language:English
Published: Elsevier 2016
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/16970/1/fist-2016-fathima-Fabrication%2C%20characterization%20and%20in%20vitro1.pdf
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author Chahal, Sugandha
Jahir Hussain, Fathima Shahitha
Kumar, Anuj
Mohammad Syaiful Bahari, Abdull Rasad
M. M., Yusoff
author_facet Chahal, Sugandha
Jahir Hussain, Fathima Shahitha
Kumar, Anuj
Mohammad Syaiful Bahari, Abdull Rasad
M. M., Yusoff
author_sort Chahal, Sugandha
collection UMP
description Development ofnovel scaffold materials that mimic the extracellular matrix, architecturally and func- tionally, is becoming highly important to meet the demands of the advances in bone tissue engineering. This paper reports, the fabrication of natural polymer cellulose derived hydroxyethyl cellulose(HEC) based nanostructured scaffolds with uniform fiber morphology through electrospinning. Poly(vinyl alcohol) (PVA) was used as anionic solvent for supporting the electrospinning of HEC. Scanning electron microscopy and ImageJ analysis revealed the formation of non-woven nanofibers with well-defined porous architecture. The interactions between HECandPVA in the electrospun nanofibers were studied by differential scanning calorimetry, X-raydiffraction, dynamic mechanical analysis thermo-gravimetric analysis; Fourier transform-infrared spectroscopy,X-ray photoelectronspectroscopy and tensiletest. The mechanical properties of scaffolds were significantly altered with different ratios of HEC/PVA. Further, the biocompatibility of HEC/PVAscaffolds was evaluated using human osteosarcomacells. TheSEM images revealed favorable cellsattachment and spreading on the nanofibrous scaffolds and MTS assay showed increased cell proliferation afterdifferent time periods. Thus, these results indicate that HEC based nanofibrous scaffolds will be a promising candidate for bone tissue engineering.
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spelling UMPir169702018-07-26T06:51:58Z http://umpir.ump.edu.my/id/eprint/16970/ Fabrication, Characterization and In Vitro Biocompatibility of Electrospun Hydroxyethyl Cellulose/poly (vinyl) Alcohol Nanofibrous Composite Biomaterial for Bone Tissue Engineering Chahal, Sugandha Jahir Hussain, Fathima Shahitha Kumar, Anuj Mohammad Syaiful Bahari, Abdull Rasad M. M., Yusoff QD Chemistry Development ofnovel scaffold materials that mimic the extracellular matrix, architecturally and func- tionally, is becoming highly important to meet the demands of the advances in bone tissue engineering. This paper reports, the fabrication of natural polymer cellulose derived hydroxyethyl cellulose(HEC) based nanostructured scaffolds with uniform fiber morphology through electrospinning. Poly(vinyl alcohol) (PVA) was used as anionic solvent for supporting the electrospinning of HEC. Scanning electron microscopy and ImageJ analysis revealed the formation of non-woven nanofibers with well-defined porous architecture. The interactions between HECandPVA in the electrospun nanofibers were studied by differential scanning calorimetry, X-raydiffraction, dynamic mechanical analysis thermo-gravimetric analysis; Fourier transform-infrared spectroscopy,X-ray photoelectronspectroscopy and tensiletest. The mechanical properties of scaffolds were significantly altered with different ratios of HEC/PVA. Further, the biocompatibility of HEC/PVAscaffolds was evaluated using human osteosarcomacells. TheSEM images revealed favorable cellsattachment and spreading on the nanofibrous scaffolds and MTS assay showed increased cell proliferation afterdifferent time periods. Thus, these results indicate that HEC based nanofibrous scaffolds will be a promising candidate for bone tissue engineering. Elsevier 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/16970/1/fist-2016-fathima-Fabrication%2C%20characterization%20and%20in%20vitro1.pdf Chahal, Sugandha and Jahir Hussain, Fathima Shahitha and Kumar, Anuj and Mohammad Syaiful Bahari, Abdull Rasad and M. M., Yusoff (2016) Fabrication, Characterization and In Vitro Biocompatibility of Electrospun Hydroxyethyl Cellulose/poly (vinyl) Alcohol Nanofibrous Composite Biomaterial for Bone Tissue Engineering. Chemical Engineering Science, 144. pp. 17-29. ISSN 0009-2509. (Published) http://dx.doi.org/10.1016/j.ces.2015.12.030 doi: 10.1016/j.ces.2015.12.030
spellingShingle QD Chemistry
Chahal, Sugandha
Jahir Hussain, Fathima Shahitha
Kumar, Anuj
Mohammad Syaiful Bahari, Abdull Rasad
M. M., Yusoff
Fabrication, Characterization and In Vitro Biocompatibility of Electrospun Hydroxyethyl Cellulose/poly (vinyl) Alcohol Nanofibrous Composite Biomaterial for Bone Tissue Engineering
title Fabrication, Characterization and In Vitro Biocompatibility of Electrospun Hydroxyethyl Cellulose/poly (vinyl) Alcohol Nanofibrous Composite Biomaterial for Bone Tissue Engineering
title_full Fabrication, Characterization and In Vitro Biocompatibility of Electrospun Hydroxyethyl Cellulose/poly (vinyl) Alcohol Nanofibrous Composite Biomaterial for Bone Tissue Engineering
title_fullStr Fabrication, Characterization and In Vitro Biocompatibility of Electrospun Hydroxyethyl Cellulose/poly (vinyl) Alcohol Nanofibrous Composite Biomaterial for Bone Tissue Engineering
title_full_unstemmed Fabrication, Characterization and In Vitro Biocompatibility of Electrospun Hydroxyethyl Cellulose/poly (vinyl) Alcohol Nanofibrous Composite Biomaterial for Bone Tissue Engineering
title_short Fabrication, Characterization and In Vitro Biocompatibility of Electrospun Hydroxyethyl Cellulose/poly (vinyl) Alcohol Nanofibrous Composite Biomaterial for Bone Tissue Engineering
title_sort fabrication characterization and in vitro biocompatibility of electrospun hydroxyethyl cellulose poly vinyl alcohol nanofibrous composite biomaterial for bone tissue engineering
topic QD Chemistry
url http://umpir.ump.edu.my/id/eprint/16970/1/fist-2016-fathima-Fabrication%2C%20characterization%20and%20in%20vitro1.pdf
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