Effects Of Agitation and Volume of Inoculum on Ferulic Acid Production by Co-Culture

Response surface methodology (RSM) has been known as the best tool in evaluating the effects of several variables and their interactions of biochemical and biotechnological processes. The main focus of this research was to study the effects of agitation and volume of inoculum in influencing the feru...

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Main Authors: Kamaliah, Abdul Samad, N., Zainol
Format: Article
Language:English
Published: Elsevier Ltd 2017
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/17439/7/Effects%20of%20agitation%20and%20volume%20of%20inoculum%20on%20ferulic%20acid%20production%20by%20co-culture%201.pdf
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author Kamaliah, Abdul Samad
N., Zainol
author_facet Kamaliah, Abdul Samad
N., Zainol
author_sort Kamaliah, Abdul Samad
collection UMP
description Response surface methodology (RSM) has been known as the best tool in evaluating the effects of several variables and their interactions of biochemical and biotechnological processes. The main focus of this research was to study the effects of agitation and volume of inoculum in influencing the ferulic acid production from banana stem waste by co-culture. The optimum conditions for the production process were also determined. RSM with five replicates at the center point was performed to evaluate the contribution of the factors. The p-values of 0.0249 and 0.0268 for agitation and volume of inoculum, respectively, indicate the importance of both factors in ferulic acid yield. Agitation at 150 rpm and volume of inoculum of 5% were found optimum in increasing the ferulic acid yield up to 510.24 mg/kg within 24 h. The results demonstrate that the use of RSM is very helpful to study the effects of interactions among the parameters for efficient production of biological products.
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spelling UMPir174392018-01-15T02:03:44Z http://umpir.ump.edu.my/id/eprint/17439/ Effects Of Agitation and Volume of Inoculum on Ferulic Acid Production by Co-Culture Kamaliah, Abdul Samad N., Zainol TP Chemical technology Response surface methodology (RSM) has been known as the best tool in evaluating the effects of several variables and their interactions of biochemical and biotechnological processes. The main focus of this research was to study the effects of agitation and volume of inoculum in influencing the ferulic acid production from banana stem waste by co-culture. The optimum conditions for the production process were also determined. RSM with five replicates at the center point was performed to evaluate the contribution of the factors. The p-values of 0.0249 and 0.0268 for agitation and volume of inoculum, respectively, indicate the importance of both factors in ferulic acid yield. Agitation at 150 rpm and volume of inoculum of 5% were found optimum in increasing the ferulic acid yield up to 510.24 mg/kg within 24 h. The results demonstrate that the use of RSM is very helpful to study the effects of interactions among the parameters for efficient production of biological products. Elsevier Ltd 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/17439/7/Effects%20of%20agitation%20and%20volume%20of%20inoculum%20on%20ferulic%20acid%20production%20by%20co-culture%201.pdf Kamaliah, Abdul Samad and N., Zainol (2017) Effects Of Agitation and Volume of Inoculum on Ferulic Acid Production by Co-Culture. Biocatalysis and Agricultural Biotechnology, 10. pp. 9-12. ISSN 1878-8181. (Published) http://doi.org/10.1016/j.bcab.2017.01.010 doi: 10.1016/j.bcab.2017.01.010
spellingShingle TP Chemical technology
Kamaliah, Abdul Samad
N., Zainol
Effects Of Agitation and Volume of Inoculum on Ferulic Acid Production by Co-Culture
title Effects Of Agitation and Volume of Inoculum on Ferulic Acid Production by Co-Culture
title_full Effects Of Agitation and Volume of Inoculum on Ferulic Acid Production by Co-Culture
title_fullStr Effects Of Agitation and Volume of Inoculum on Ferulic Acid Production by Co-Culture
title_full_unstemmed Effects Of Agitation and Volume of Inoculum on Ferulic Acid Production by Co-Culture
title_short Effects Of Agitation and Volume of Inoculum on Ferulic Acid Production by Co-Culture
title_sort effects of agitation and volume of inoculum on ferulic acid production by co culture
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/17439/7/Effects%20of%20agitation%20and%20volume%20of%20inoculum%20on%20ferulic%20acid%20production%20by%20co-culture%201.pdf
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AT nzainol effectsofagitationandvolumeofinoculumonferulicacidproductionbycoculture