A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation
This article presents the comparison of reusability and leakage between entrapped and covalently bonded laccase and their performances towards the selective oxidation of glycerol. The reusability of immobilized laccase enzyme was studied by reacting a batch of immobilized laccase with ABTS for 15 cy...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis
2018
|
| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/19265/7/A%20comparison%20of%20entrapped%20and%20covalently%20bonded1.pdf |
| _version_ | 1825811788863635456 |
|---|---|
| author | Hong, Chi Shein Lau Cindy, Chin Yee Leong, Chun Yi Chua, Gek Kee Chin, S. Y. |
| author_facet | Hong, Chi Shein Lau Cindy, Chin Yee Leong, Chun Yi Chua, Gek Kee Chin, S. Y. |
| author_sort | Hong, Chi Shein |
| collection | UMP |
| description | This article presents the comparison of reusability and leakage between entrapped and covalently bonded laccase and their performances towards the selective oxidation of glycerol. The reusability of immobilized laccase enzyme was studied by reacting a batch of immobilized laccase with ABTS for 15 cycles. The investigation of the leakage of immobilized laccase was carried out by storing the immobilized laccase in acetate buffer solution for 32 days. The data show that the retained enzyme activities of entrapped and covalently bonded enzyme after being reused for eight cycles were well above 60% and the leakages after storing for a month in the acetate buffer at 4C were well below 15%. The entrapped laccase coupled with TEMPO was found to perform better and gave a two-fold higher yield of glyceraldehyde and glyceric acid in the selective oxidation of glycerol compared to covalently bonded laccase. Hence, physical entrapment of laccase would be a suitable immobilization method in the laccase-mediated selective oxidation of glycerol. |
| first_indexed | 2024-03-06T12:19:31Z |
| format | Article |
| id | UMPir19265 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:19:31Z |
| publishDate | 2018 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | UMPir192652019-01-18T03:08:16Z http://umpir.ump.edu.my/id/eprint/19265/ A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation Hong, Chi Shein Lau Cindy, Chin Yee Leong, Chun Yi Chua, Gek Kee Chin, S. Y. QD Chemistry TP Chemical technology This article presents the comparison of reusability and leakage between entrapped and covalently bonded laccase and their performances towards the selective oxidation of glycerol. The reusability of immobilized laccase enzyme was studied by reacting a batch of immobilized laccase with ABTS for 15 cycles. The investigation of the leakage of immobilized laccase was carried out by storing the immobilized laccase in acetate buffer solution for 32 days. The data show that the retained enzyme activities of entrapped and covalently bonded enzyme after being reused for eight cycles were well above 60% and the leakages after storing for a month in the acetate buffer at 4C were well below 15%. The entrapped laccase coupled with TEMPO was found to perform better and gave a two-fold higher yield of glyceraldehyde and glyceric acid in the selective oxidation of glycerol compared to covalently bonded laccase. Hence, physical entrapment of laccase would be a suitable immobilization method in the laccase-mediated selective oxidation of glycerol. Taylor & Francis 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/19265/7/A%20comparison%20of%20entrapped%20and%20covalently%20bonded1.pdf Hong, Chi Shein and Lau Cindy, Chin Yee and Leong, Chun Yi and Chua, Gek Kee and Chin, S. Y. (2018) A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation. Biocatalysis and Biotransformation, 36 (5). pp. 352-361. ISSN 1024-2422 (Print); 1029-2446 (Online). (Published) https://doi.org/10.1080/10242422.2017.1384467 10.1080/10242422.2017.1384467 |
| spellingShingle | QD Chemistry TP Chemical technology Hong, Chi Shein Lau Cindy, Chin Yee Leong, Chun Yi Chua, Gek Kee Chin, S. Y. A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation |
| title | A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation |
| title_full | A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation |
| title_fullStr | A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation |
| title_full_unstemmed | A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation |
| title_short | A comparison of entrapped and covalently bonded laccase: Study of its leakage, reusability, and the catalytic efficiency in TEMPO-mediated glycerol oxidation |
| title_sort | comparison of entrapped and covalently bonded laccase study of its leakage reusability and the catalytic efficiency in tempo mediated glycerol oxidation |
| topic | QD Chemistry TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/19265/7/A%20comparison%20of%20entrapped%20and%20covalently%20bonded1.pdf |
| work_keys_str_mv | AT hongchishein acomparisonofentrappedandcovalentlybondedlaccasestudyofitsleakagereusabilityandthecatalyticefficiencyintempomediatedglyceroloxidation AT laucindychinyee acomparisonofentrappedandcovalentlybondedlaccasestudyofitsleakagereusabilityandthecatalyticefficiencyintempomediatedglyceroloxidation AT leongchunyi acomparisonofentrappedandcovalentlybondedlaccasestudyofitsleakagereusabilityandthecatalyticefficiencyintempomediatedglyceroloxidation AT chuagekkee acomparisonofentrappedandcovalentlybondedlaccasestudyofitsleakagereusabilityandthecatalyticefficiencyintempomediatedglyceroloxidation AT chinsy acomparisonofentrappedandcovalentlybondedlaccasestudyofitsleakagereusabilityandthecatalyticefficiencyintempomediatedglyceroloxidation AT hongchishein comparisonofentrappedandcovalentlybondedlaccasestudyofitsleakagereusabilityandthecatalyticefficiencyintempomediatedglyceroloxidation AT laucindychinyee comparisonofentrappedandcovalentlybondedlaccasestudyofitsleakagereusabilityandthecatalyticefficiencyintempomediatedglyceroloxidation AT leongchunyi comparisonofentrappedandcovalentlybondedlaccasestudyofitsleakagereusabilityandthecatalyticefficiencyintempomediatedglyceroloxidation AT chuagekkee comparisonofentrappedandcovalentlybondedlaccasestudyofitsleakagereusabilityandthecatalyticefficiencyintempomediatedglyceroloxidation AT chinsy comparisonofentrappedandcovalentlybondedlaccasestudyofitsleakagereusabilityandthecatalyticefficiencyintempomediatedglyceroloxidation |