Biomolecular Aspects of Second Order Limit Language

The study on the recombinant behavior of double-stranded DNA molecules has led to the mathematical modelling of DNA splicing system. The interdisciplinary study is founded from the knowledge of informational macromolecules and formal language theory. A splicing language is resulted from a splicing s...

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Main Authors: Muhammad Azrin, Ahmad, Nor Haniza, Sarmin, Mohd Firdaus, Abdul-Wahab, Fong, Wan Heng, Yuhani, Yusof
Format: Article
Language:English
Published: Penerbit UTM Press 2018
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/20041/1/Biomolecular%20Aspects%20of%20Second%20Order%20Limit%20Language-fist-2018.pdf
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author Muhammad Azrin, Ahmad
Nor Haniza, Sarmin
Mohd Firdaus, Abdul-Wahab
Fong, Wan Heng
Yuhani, Yusof
author_facet Muhammad Azrin, Ahmad
Nor Haniza, Sarmin
Mohd Firdaus, Abdul-Wahab
Fong, Wan Heng
Yuhani, Yusof
author_sort Muhammad Azrin, Ahmad
collection UMP
description The study on the recombinant behavior of double-stranded DNA molecules has led to the mathematical modelling of DNA splicing system. The interdisciplinary study is founded from the knowledge of informational macromolecules and formal language theory. A splicing language is resulted from a splicing system, in which different types of splicing langauges have been researched previous, namely adult/inert, transcient and first order limit language. Recently, second order limit language has been extensively explored from the first order limit language. Therefore, in this paper, a laboratory experiment was conducted to validate the existence of a second order limit language. To accomplish it, an initial strand of double-stranded DNA, amplified from bacteriophage lambda, was generated through polymerase chain reaction to generate thousands of copies of double-stranded DNA molecules. A restriction enzyme and ligase were added to the solution to complete the reaction. The reaction mixture was then subjected to polyacrylamide gel electrophoresis to separate biological macromolecules according to their sizes. A mathematical model derived at the early study was used to predict the approximate length of each string in the splicing language. The results obtained from the experiment are then used to verify the mathematical model of a second order limit language. This study shows that the theory on the second order limit language is biologically proven hence the model has been validated.
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spelling UMPir200412018-07-18T08:33:01Z http://umpir.ump.edu.my/id/eprint/20041/ Biomolecular Aspects of Second Order Limit Language Muhammad Azrin, Ahmad Nor Haniza, Sarmin Mohd Firdaus, Abdul-Wahab Fong, Wan Heng Yuhani, Yusof QA Mathematics The study on the recombinant behavior of double-stranded DNA molecules has led to the mathematical modelling of DNA splicing system. The interdisciplinary study is founded from the knowledge of informational macromolecules and formal language theory. A splicing language is resulted from a splicing system, in which different types of splicing langauges have been researched previous, namely adult/inert, transcient and first order limit language. Recently, second order limit language has been extensively explored from the first order limit language. Therefore, in this paper, a laboratory experiment was conducted to validate the existence of a second order limit language. To accomplish it, an initial strand of double-stranded DNA, amplified from bacteriophage lambda, was generated through polymerase chain reaction to generate thousands of copies of double-stranded DNA molecules. A restriction enzyme and ligase were added to the solution to complete the reaction. The reaction mixture was then subjected to polyacrylamide gel electrophoresis to separate biological macromolecules according to their sizes. A mathematical model derived at the early study was used to predict the approximate length of each string in the splicing language. The results obtained from the experiment are then used to verify the mathematical model of a second order limit language. This study shows that the theory on the second order limit language is biologically proven hence the model has been validated. Penerbit UTM Press 2018 Article PeerReviewed application/pdf en cc_by_nc http://umpir.ump.edu.my/id/eprint/20041/1/Biomolecular%20Aspects%20of%20Second%20Order%20Limit%20Language-fist-2018.pdf Muhammad Azrin, Ahmad and Nor Haniza, Sarmin and Mohd Firdaus, Abdul-Wahab and Fong, Wan Heng and Yuhani, Yusof (2018) Biomolecular Aspects of Second Order Limit Language. Malaysian Journal of Fundamental and Applied Sciences, 14 (1). pp. 15-19. ISSN 2289-599X. (Published) https://mjfas.utm.my/index.php/mjfas/article/view/727
spellingShingle QA Mathematics
Muhammad Azrin, Ahmad
Nor Haniza, Sarmin
Mohd Firdaus, Abdul-Wahab
Fong, Wan Heng
Yuhani, Yusof
Biomolecular Aspects of Second Order Limit Language
title Biomolecular Aspects of Second Order Limit Language
title_full Biomolecular Aspects of Second Order Limit Language
title_fullStr Biomolecular Aspects of Second Order Limit Language
title_full_unstemmed Biomolecular Aspects of Second Order Limit Language
title_short Biomolecular Aspects of Second Order Limit Language
title_sort biomolecular aspects of second order limit language
topic QA Mathematics
url http://umpir.ump.edu.my/id/eprint/20041/1/Biomolecular%20Aspects%20of%20Second%20Order%20Limit%20Language-fist-2018.pdf
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AT fongwanheng biomolecularaspectsofsecondorderlimitlanguage
AT yuhaniyusof biomolecularaspectsofsecondorderlimitlanguage