Study of enzyme purification method and growth pattern for Pseudomonas putida in mercury removal

Mercury is one of the toxic element and very harmful. The exposure of the mercury will mainly cause health effect and the exposure can be in term of dose, age of person exposed, duration exposed route exposed and duration of exposed. In Malaysia, Mercury is one of the heavy metals of concern, found...

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Main Authors: Abdul Aziz, Mohd Azoddein, Siti Nur Baizura, Yusuf, Faten Ahada, Mohd Azli, Ahmad Bazli, Bustary, Mariah, Che Mamat, Nur Athirah, Mohammad Basir
Format: Conference or Workshop Item
Language:English
Published: 2018
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/21401/1/Study%20of%20enzyme%20purification%20method%20and%20growth%20pattern.pdf
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author Abdul Aziz, Mohd Azoddein
Siti Nur Baizura, Yusuf
Faten Ahada, Mohd Azli
Ahmad Bazli, Bustary
Mariah, Che Mamat
Nur Athirah, Mohammad Basir
author_facet Abdul Aziz, Mohd Azoddein
Siti Nur Baizura, Yusuf
Faten Ahada, Mohd Azli
Ahmad Bazli, Bustary
Mariah, Che Mamat
Nur Athirah, Mohammad Basir
author_sort Abdul Aziz, Mohd Azoddein
collection UMP
description Mercury is one of the toxic element and very harmful. The exposure of the mercury will mainly cause health effect and the exposure can be in term of dose, age of person exposed, duration exposed route exposed and duration of exposed. In Malaysia, Mercury is one of the heavy metals of concern, found in wastewaters coming from oil refinery and petrochemical industries. Mercury and mercurial compounds are highly toxic contaminants in the aquatic systems and soils. The proper disposal of wastewater should be implement with the Department of Environment of Malaysia (DOE) which is the concentration of mercury should be 0.05 ppm for standard B in wastewater for the sake of people and environment health. There are many types of mercury removal technology to reduce the concentration of mercury such as chemical treatment, ion exchange, membrane filtration, adsorption as well as bioremediation which used for mercury removal in this study. This paper present the activity of enzyme from Pseudomonas putida (P. putida) is produced in the mercury treatment from petrochemical wastewater at optimum condition. P. putida growth at optimum condition in mercury different concentration was observed. Conclusion optimum condition concentration of mercury and shaker speed at 7 ppm is 41 ˚C and 180 rpm respectively, with able to reduce 92.59 % mercury concentration. During the processes of mercury removal, mercuric reductase was produced at value of 56 kDa.
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spelling UMPir214012018-06-29T03:57:59Z http://umpir.ump.edu.my/id/eprint/21401/ Study of enzyme purification method and growth pattern for Pseudomonas putida in mercury removal Abdul Aziz, Mohd Azoddein Siti Nur Baizura, Yusuf Faten Ahada, Mohd Azli Ahmad Bazli, Bustary Mariah, Che Mamat Nur Athirah, Mohammad Basir TP Chemical technology Mercury is one of the toxic element and very harmful. The exposure of the mercury will mainly cause health effect and the exposure can be in term of dose, age of person exposed, duration exposed route exposed and duration of exposed. In Malaysia, Mercury is one of the heavy metals of concern, found in wastewaters coming from oil refinery and petrochemical industries. Mercury and mercurial compounds are highly toxic contaminants in the aquatic systems and soils. The proper disposal of wastewater should be implement with the Department of Environment of Malaysia (DOE) which is the concentration of mercury should be 0.05 ppm for standard B in wastewater for the sake of people and environment health. There are many types of mercury removal technology to reduce the concentration of mercury such as chemical treatment, ion exchange, membrane filtration, adsorption as well as bioremediation which used for mercury removal in this study. This paper present the activity of enzyme from Pseudomonas putida (P. putida) is produced in the mercury treatment from petrochemical wastewater at optimum condition. P. putida growth at optimum condition in mercury different concentration was observed. Conclusion optimum condition concentration of mercury and shaker speed at 7 ppm is 41 ˚C and 180 rpm respectively, with able to reduce 92.59 % mercury concentration. During the processes of mercury removal, mercuric reductase was produced at value of 56 kDa. 2018 Conference or Workshop Item NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/21401/1/Study%20of%20enzyme%20purification%20method%20and%20growth%20pattern.pdf Abdul Aziz, Mohd Azoddein and Siti Nur Baizura, Yusuf and Faten Ahada, Mohd Azli and Ahmad Bazli, Bustary and Mariah, Che Mamat and Nur Athirah, Mohammad Basir (2018) Study of enzyme purification method and growth pattern for Pseudomonas putida in mercury removal. In: 5th International Conference On “Trends In Multidisciplinary Business & Economic Research” (TMBER -2018) , 23-24 March 2018 , Rydges Camperdown, Sydney Australia. pp. 1-6.. (Unpublished)
spellingShingle TP Chemical technology
Abdul Aziz, Mohd Azoddein
Siti Nur Baizura, Yusuf
Faten Ahada, Mohd Azli
Ahmad Bazli, Bustary
Mariah, Che Mamat
Nur Athirah, Mohammad Basir
Study of enzyme purification method and growth pattern for Pseudomonas putida in mercury removal
title Study of enzyme purification method and growth pattern for Pseudomonas putida in mercury removal
title_full Study of enzyme purification method and growth pattern for Pseudomonas putida in mercury removal
title_fullStr Study of enzyme purification method and growth pattern for Pseudomonas putida in mercury removal
title_full_unstemmed Study of enzyme purification method and growth pattern for Pseudomonas putida in mercury removal
title_short Study of enzyme purification method and growth pattern for Pseudomonas putida in mercury removal
title_sort study of enzyme purification method and growth pattern for pseudomonas putida in mercury removal
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/21401/1/Study%20of%20enzyme%20purification%20method%20and%20growth%20pattern.pdf
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