Direct Electrochemistry of Catalase Immobilized at Polymerized-SnO2 Multiporous Modified Electrode for an Amperometric H2O2 Biosensor
A novel amperometric H2O2 biosensor based on immobilization of catalase with polymerized MPNFs of SnO2 onto glassy carbon electrode with chitosan have been proposed in this work. Multiporous nanofibers of SnO2 were synthesized by electrospinning method from the tin precursor by controlling the conce...
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| Format: | Article |
| Language: | English |
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Biomedical Research Network
2018
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| Online Access: | http://umpir.ump.edu.my/id/eprint/21472/1/Direct%20Electrochemistry%20of%20Catalase%20Immobilized.pdf |
| _version_ | 1825812133018861568 |
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| author | Alim, Samiul Jaya Vejayan, Palliah A. K. M., Kafi |
| author_facet | Alim, Samiul Jaya Vejayan, Palliah A. K. M., Kafi |
| author_sort | Alim, Samiul |
| collection | UMP |
| description | A novel amperometric H2O2 biosensor based on immobilization of catalase with polymerized MPNFs of SnO2 onto glassy carbon electrode with chitosan have been proposed in this work. Multiporous nanofibers of SnO2 were synthesized by electrospinning method from the tin precursor by controlling the concentration followed by polymerized with aniline. Catalase was then co-immobilized with the polymerized nanofibers on the surface of glassy carbon electrode by using chitosan. The polymerized MPNFs of SnO2 play a significant role in facilitating the electron exchange between the electroactive center of catalase and the electrode surface. Cyclic Voltammetry and amperometry were used to study and optimize the performance of the fabricated H2O2 biosensor. The PANI/SnO2-NFs/Catalase/Ch/GCE biosensor displayed a linear amperometric response towards the H2O2 concentration range from 10 to 120M with a detection limit of 0.6M (based on S/N=3). Furthermore, the biosensor reported in this work exhibited acceptable stability, reproducibility, and repeatability. |
| first_indexed | 2024-03-06T12:24:37Z |
| format | Article |
| id | UMPir21472 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:24:37Z |
| publishDate | 2018 |
| publisher | Biomedical Research Network |
| record_format | dspace |
| spelling | UMPir214722018-07-03T03:31:43Z http://umpir.ump.edu.my/id/eprint/21472/ Direct Electrochemistry of Catalase Immobilized at Polymerized-SnO2 Multiporous Modified Electrode for an Amperometric H2O2 Biosensor Alim, Samiul Jaya Vejayan, Palliah A. K. M., Kafi QD Chemistry A novel amperometric H2O2 biosensor based on immobilization of catalase with polymerized MPNFs of SnO2 onto glassy carbon electrode with chitosan have been proposed in this work. Multiporous nanofibers of SnO2 were synthesized by electrospinning method from the tin precursor by controlling the concentration followed by polymerized with aniline. Catalase was then co-immobilized with the polymerized nanofibers on the surface of glassy carbon electrode by using chitosan. The polymerized MPNFs of SnO2 play a significant role in facilitating the electron exchange between the electroactive center of catalase and the electrode surface. Cyclic Voltammetry and amperometry were used to study and optimize the performance of the fabricated H2O2 biosensor. The PANI/SnO2-NFs/Catalase/Ch/GCE biosensor displayed a linear amperometric response towards the H2O2 concentration range from 10 to 120M with a detection limit of 0.6M (based on S/N=3). Furthermore, the biosensor reported in this work exhibited acceptable stability, reproducibility, and repeatability. Biomedical Research Network 2018 Article PeerReviewed text en cc_by_4 http://umpir.ump.edu.my/id/eprint/21472/1/Direct%20Electrochemistry%20of%20Catalase%20Immobilized.pdf Alim, Samiul and Jaya Vejayan, Palliah and A. K. M., Kafi (2018) Direct Electrochemistry of Catalase Immobilized at Polymerized-SnO2 Multiporous Modified Electrode for an Amperometric H2O2 Biosensor. Biomedical Journal of Scientific and Technical Research, 3 (4). pp. 1-6. ISSN 2574-1241. (Published) https://biomedres.us/pdfs/BJSTR.MS.ID.000942.pdf DOI: 10.26717/BJSTR.2018.03.000942 |
| spellingShingle | QD Chemistry Alim, Samiul Jaya Vejayan, Palliah A. K. M., Kafi Direct Electrochemistry of Catalase Immobilized at Polymerized-SnO2 Multiporous Modified Electrode for an Amperometric H2O2 Biosensor |
| title | Direct Electrochemistry of Catalase Immobilized at Polymerized-SnO2 Multiporous Modified Electrode for an Amperometric H2O2 Biosensor |
| title_full | Direct Electrochemistry of Catalase Immobilized at Polymerized-SnO2 Multiporous Modified Electrode for an Amperometric H2O2 Biosensor |
| title_fullStr | Direct Electrochemistry of Catalase Immobilized at Polymerized-SnO2 Multiporous Modified Electrode for an Amperometric H2O2 Biosensor |
| title_full_unstemmed | Direct Electrochemistry of Catalase Immobilized at Polymerized-SnO2 Multiporous Modified Electrode for an Amperometric H2O2 Biosensor |
| title_short | Direct Electrochemistry of Catalase Immobilized at Polymerized-SnO2 Multiporous Modified Electrode for an Amperometric H2O2 Biosensor |
| title_sort | direct electrochemistry of catalase immobilized at polymerized sno2 multiporous modified electrode for an amperometric h2o2 biosensor |
| topic | QD Chemistry |
| url | http://umpir.ump.edu.my/id/eprint/21472/1/Direct%20Electrochemistry%20of%20Catalase%20Immobilized.pdf |
| work_keys_str_mv | AT alimsamiul directelectrochemistryofcatalaseimmobilizedatpolymerizedsno2multiporousmodifiedelectrodeforanamperometrich2o2biosensor AT jayavejayanpalliah directelectrochemistryofcatalaseimmobilizedatpolymerizedsno2multiporousmodifiedelectrodeforanamperometrich2o2biosensor AT akmkafi directelectrochemistryofcatalaseimmobilizedatpolymerizedsno2multiporousmodifiedelectrodeforanamperometrich2o2biosensor |