Characterization and effect of extraction solvents on the yield and total phenolic content from Vernonia amygdalina leaves

In this study, the effects of extraction solvents on the yield and total phenolic content of Vernonia amygdalina leaf were examined. The considered solvents were water, 20% v/v ethanol, 40% v/v ethanol, 60% v/v ethanol, 80% v/v ethanol, and 100% v/v ethanol using Soxhlet extraction technique. Highes...

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Main Authors: Alara, Oluwaseun Ruth, Nour, A. H., Siti Kholijah, Abdul Mudalip, Olalere, Olusegun Abayomi
Format: Article
Language:English
Published: Springer International Publishing 2018
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/21923/7/Characterization%20and%20effect%20of%20extraction1.pdf
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author Alara, Oluwaseun Ruth
Nour, A. H.
Siti Kholijah, Abdul Mudalip
Olalere, Olusegun Abayomi
author_facet Alara, Oluwaseun Ruth
Nour, A. H.
Siti Kholijah, Abdul Mudalip
Olalere, Olusegun Abayomi
author_sort Alara, Oluwaseun Ruth
collection UMP
description In this study, the effects of extraction solvents on the yield and total phenolic content of Vernonia amygdalina leaf were examined. The considered solvents were water, 20% v/v ethanol, 40% v/v ethanol, 60% v/v ethanol, 80% v/v ethanol, and 100% v/v ethanol using Soxhlet extraction technique. Highest extraction yield (19.45 ± 0.22% g/g) and total phenolic content (96.25 ± 2.52 mg GAE/g d.w.) were achieved using 60% v/v aqueous ethanol solution. In addition, there exist a significant correlation between total phenolic content and antioxidant activities using DPPH (R2 = 0.9397, p < 0.05) and ABTS scavenging activity (R2 = 0.7700, p < 0.05). More so, the FTIR analysis confirmed the presence of functional groups attributed to its antioxidant property. There was a significant damage in the cell wall of V. amygdalina leaf after extracted with 60% v/v ethanol solution. Therefore, V. amygdalina leaf can be a good source of antioxidants.
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spelling UMPir219232019-09-10T04:02:53Z http://umpir.ump.edu.my/id/eprint/21923/ Characterization and effect of extraction solvents on the yield and total phenolic content from Vernonia amygdalina leaves Alara, Oluwaseun Ruth Nour, A. H. Siti Kholijah, Abdul Mudalip Olalere, Olusegun Abayomi TP Chemical technology In this study, the effects of extraction solvents on the yield and total phenolic content of Vernonia amygdalina leaf were examined. The considered solvents were water, 20% v/v ethanol, 40% v/v ethanol, 60% v/v ethanol, 80% v/v ethanol, and 100% v/v ethanol using Soxhlet extraction technique. Highest extraction yield (19.45 ± 0.22% g/g) and total phenolic content (96.25 ± 2.52 mg GAE/g d.w.) were achieved using 60% v/v aqueous ethanol solution. In addition, there exist a significant correlation between total phenolic content and antioxidant activities using DPPH (R2 = 0.9397, p < 0.05) and ABTS scavenging activity (R2 = 0.7700, p < 0.05). More so, the FTIR analysis confirmed the presence of functional groups attributed to its antioxidant property. There was a significant damage in the cell wall of V. amygdalina leaf after extracted with 60% v/v ethanol solution. Therefore, V. amygdalina leaf can be a good source of antioxidants. Springer International Publishing 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/21923/7/Characterization%20and%20effect%20of%20extraction1.pdf Alara, Oluwaseun Ruth and Nour, A. H. and Siti Kholijah, Abdul Mudalip and Olalere, Olusegun Abayomi (2018) Characterization and effect of extraction solvents on the yield and total phenolic content from Vernonia amygdalina leaves. Journal of Food Measurement and Characterization, 12 (1). pp. 311-316. ISSN 2193-4126. (Published) https://doi.org/10.1007/s11694-017-9642-y https://doi.org/10.1007/s11694-017-9642-y
spellingShingle TP Chemical technology
Alara, Oluwaseun Ruth
Nour, A. H.
Siti Kholijah, Abdul Mudalip
Olalere, Olusegun Abayomi
Characterization and effect of extraction solvents on the yield and total phenolic content from Vernonia amygdalina leaves
title Characterization and effect of extraction solvents on the yield and total phenolic content from Vernonia amygdalina leaves
title_full Characterization and effect of extraction solvents on the yield and total phenolic content from Vernonia amygdalina leaves
title_fullStr Characterization and effect of extraction solvents on the yield and total phenolic content from Vernonia amygdalina leaves
title_full_unstemmed Characterization and effect of extraction solvents on the yield and total phenolic content from Vernonia amygdalina leaves
title_short Characterization and effect of extraction solvents on the yield and total phenolic content from Vernonia amygdalina leaves
title_sort characterization and effect of extraction solvents on the yield and total phenolic content from vernonia amygdalina leaves
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/21923/7/Characterization%20and%20effect%20of%20extraction1.pdf
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