Microbial alkaline protease from animal waste: Immobilization and characterization

Alkaline protease (AP) produced from protein-rich animal waste as chicken feather (CF) and fish fin (FF) by mixed culture was immobilized and characterized. The immobilized enzyme activity obtained in 0.25g beads was 0.398 and 0.411 (U/mL) for CF and FF respectively. Besides; the initial activity wa...

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Main Authors: Raid D., Thanoon, Makky, Essam A., M. M., Yusoff
Format: Conference or Workshop Item
Language:English
English
Published: 2018
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/21975/1/25.%20Microbial%20alkaline%20protease%20from%20animal%20waste%20Immobilization.pdf
http://umpir.ump.edu.my/id/eprint/21975/2/25.1%20Microbial%20alkaline%20protease%20from%20animal%20waste%20Immobilization.pdf
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author Raid D., Thanoon
Makky, Essam A.
M. M., Yusoff
author_facet Raid D., Thanoon
Makky, Essam A.
M. M., Yusoff
author_sort Raid D., Thanoon
collection UMP
description Alkaline protease (AP) produced from protein-rich animal waste as chicken feather (CF) and fish fin (FF) by mixed culture was immobilized and characterized. The immobilized enzyme activity obtained in 0.25g beads was 0.398 and 0.411 (U/mL) for CF and FF respectively. Besides; the initial activity was 0.424±0.011 and 0.417±0.017 (U/mL) from the extracted protein, accordingly. While enzyme activity entrapped in 0.25g beads was 0.081 and 0.151 (U/ml), respectively. The beads protein content at 4 cycles up to 0.171±0.001 and 0.176±0.006 (mg/ml), respectively and gradually decreased through the second run by approximately 10% and ended up with 40% activity loss 0.124±0.002 and 0.112±0.003 (mg/ml) accordingly. The enzyme showed zero % activity loss for the first cycle and gradually decreased along the fourth cycle. The storage stability of the enzyme was recorded at 1st day 0.424 ± 0.011 and 0.417 ± 0.017 (U/mL) as the initial activity of AP, respectively, while the activity for the 5th day 0.167 ± 0.004 and 0.167 ± 0.003 (U/mL) were used as the final activity of the immobilized and free enzymes, respectively.
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spelling UMPir219752018-09-21T02:08:27Z http://umpir.ump.edu.my/id/eprint/21975/ Microbial alkaline protease from animal waste: Immobilization and characterization Raid D., Thanoon Makky, Essam A. M. M., Yusoff QD Chemistry Alkaline protease (AP) produced from protein-rich animal waste as chicken feather (CF) and fish fin (FF) by mixed culture was immobilized and characterized. The immobilized enzyme activity obtained in 0.25g beads was 0.398 and 0.411 (U/mL) for CF and FF respectively. Besides; the initial activity was 0.424±0.011 and 0.417±0.017 (U/mL) from the extracted protein, accordingly. While enzyme activity entrapped in 0.25g beads was 0.081 and 0.151 (U/ml), respectively. The beads protein content at 4 cycles up to 0.171±0.001 and 0.176±0.006 (mg/ml), respectively and gradually decreased through the second run by approximately 10% and ended up with 40% activity loss 0.124±0.002 and 0.112±0.003 (mg/ml) accordingly. The enzyme showed zero % activity loss for the first cycle and gradually decreased along the fourth cycle. The storage stability of the enzyme was recorded at 1st day 0.424 ± 0.011 and 0.417 ± 0.017 (U/mL) as the initial activity of AP, respectively, while the activity for the 5th day 0.167 ± 0.004 and 0.167 ± 0.003 (U/mL) were used as the final activity of the immobilized and free enzymes, respectively. 2018-03 Conference or Workshop Item NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/21975/1/25.%20Microbial%20alkaline%20protease%20from%20animal%20waste%20Immobilization.pdf pdf en http://umpir.ump.edu.my/id/eprint/21975/2/25.1%20Microbial%20alkaline%20protease%20from%20animal%20waste%20Immobilization.pdf Raid D., Thanoon and Makky, Essam A. and M. M., Yusoff (2018) Microbial alkaline protease from animal waste: Immobilization and characterization. In: The 2nd International Conference On Sustainable Energy Engineering (ICSEE 2018) , 15 - 17 Jun 2018 , Sydney, Australia. pp. 1-5.. (Unpublished) (Unpublished)
spellingShingle QD Chemistry
Raid D., Thanoon
Makky, Essam A.
M. M., Yusoff
Microbial alkaline protease from animal waste: Immobilization and characterization
title Microbial alkaline protease from animal waste: Immobilization and characterization
title_full Microbial alkaline protease from animal waste: Immobilization and characterization
title_fullStr Microbial alkaline protease from animal waste: Immobilization and characterization
title_full_unstemmed Microbial alkaline protease from animal waste: Immobilization and characterization
title_short Microbial alkaline protease from animal waste: Immobilization and characterization
title_sort microbial alkaline protease from animal waste immobilization and characterization
topic QD Chemistry
url http://umpir.ump.edu.my/id/eprint/21975/1/25.%20Microbial%20alkaline%20protease%20from%20animal%20waste%20Immobilization.pdf
http://umpir.ump.edu.my/id/eprint/21975/2/25.1%20Microbial%20alkaline%20protease%20from%20animal%20waste%20Immobilization.pdf
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AT mmyusoff microbialalkalineproteasefromanimalwasteimmobilizationandcharacterization