Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane
Cyclodextrin glucanotransferase (CGTase) is a starch degrading enzyme to produce cyclodextrin (CD), which can encapsulate compounds to remove flavors and aroma. However, the use of the enzyme is often limited due to the instability of the enzyme resulting in low production of CD. Immobilization tech...
| Main Authors: | , , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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IOP Publishing Ltd.
2018
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| Online Access: | http://umpir.ump.edu.my/id/eprint/22620/1/Stability%20and%20reusability%20of%20cyclodextrin%20glucanotransferase%20immobilized%20on%20hollow%20fiber%20membrane.pdf |
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| author | S., Suhaimi Rohaida, Che Man N., Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Aizi Nor Mazila, Ramli |
| author_facet | S., Suhaimi Rohaida, Che Man N., Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Aizi Nor Mazila, Ramli |
| author_sort | S., Suhaimi |
| collection | UMP |
| description | Cyclodextrin glucanotransferase (CGTase) is a starch degrading enzyme to produce cyclodextrin (CD), which can encapsulate compounds to remove flavors and aroma. However, the use of the enzyme is often limited due to the instability of the enzyme resulting in low production of CD. Immobilization technique has greatly improved the characteristics of the enzyme during the reaction. In this study, CGTase was immobilized on polyvinylidene difluoride (PVDF) hollow fiber membrane by adsorption method. The stability and reusability of the immobilized CGTase were studied and compared with free form of the CGTase. Thermal stability of the immobilized CGTase able to retain 50% of the initial residual activity at temperature up to 70°C. While there was an improvement in pH stability with a wider pH range from pH 5 to pH 8. The reusability of the immobilized CGTase was able to retain up to 40% of the initial CD production after repeatedly used for 10 cycles. Hence, the immobilization of CGTase shows a good physical and chemical resistance, which may be advantageous to be applied in industry. |
| first_indexed | 2024-03-06T12:27:33Z |
| format | Conference or Workshop Item |
| id | UMPir22620 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:27:33Z |
| publishDate | 2018 |
| publisher | IOP Publishing Ltd. |
| record_format | dspace |
| spelling | UMPir226202018-12-14T03:07:20Z http://umpir.ump.edu.my/id/eprint/22620/ Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane S., Suhaimi Rohaida, Che Man N., Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Aizi Nor Mazila, Ramli Q Science (General) TP Chemical technology Cyclodextrin glucanotransferase (CGTase) is a starch degrading enzyme to produce cyclodextrin (CD), which can encapsulate compounds to remove flavors and aroma. However, the use of the enzyme is often limited due to the instability of the enzyme resulting in low production of CD. Immobilization technique has greatly improved the characteristics of the enzyme during the reaction. In this study, CGTase was immobilized on polyvinylidene difluoride (PVDF) hollow fiber membrane by adsorption method. The stability and reusability of the immobilized CGTase were studied and compared with free form of the CGTase. Thermal stability of the immobilized CGTase able to retain 50% of the initial residual activity at temperature up to 70°C. While there was an improvement in pH stability with a wider pH range from pH 5 to pH 8. The reusability of the immobilized CGTase was able to retain up to 40% of the initial CD production after repeatedly used for 10 cycles. Hence, the immobilization of CGTase shows a good physical and chemical resistance, which may be advantageous to be applied in industry. IOP Publishing Ltd. 2018 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22620/1/Stability%20and%20reusability%20of%20cyclodextrin%20glucanotransferase%20immobilized%20on%20hollow%20fiber%20membrane.pdf S., Suhaimi and Rohaida, Che Man and N., Jamil and Zatul Iffah, Mohd Arshad and Shalyda, Md Shaarani and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman and Aizi Nor Mazila, Ramli (2018) Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane. In: 4th International Conference on Agricultural and Biological Sciences (ABS 2018) , 26-29 June 2018 , Hangzhou, Zhejiang Province, China. pp. 1-7., 185 (1). ISSN 1755-1307 (Published) http://iopscience.iop.org/article/10.1088/1755-1315/185/1/012002/meta |
| spellingShingle | Q Science (General) TP Chemical technology S., Suhaimi Rohaida, Che Man N., Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Aizi Nor Mazila, Ramli Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane |
| title | Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane |
| title_full | Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane |
| title_fullStr | Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane |
| title_full_unstemmed | Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane |
| title_short | Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane |
| title_sort | stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane |
| topic | Q Science (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/22620/1/Stability%20and%20reusability%20of%20cyclodextrin%20glucanotransferase%20immobilized%20on%20hollow%20fiber%20membrane.pdf |
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