Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme
The production of cyclodextrin (CD) over the years has been increasing due to the numerous applications in industries such as in food, cosmetic, pharmaceutical and agricultural industries. However, cyclodextrin glucanotransferase (CGTase) which involved in the enzymatic reaction on the production of...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Teknologi Malaysia
2018
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| Online Access: | http://umpir.ump.edu.my/id/eprint/22866/1/Effect%20of%20reaction%20conditions%20on%20the%20synthesis%20of%20cyclodextrin%20%28CD%29%20by%20using%20immobilized%20enzyme.pdf |
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| author | Suhaily, Suhaimi Rohaida, Che Man Natassha, Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Aizi Nor Mazila, Ramli |
| author_facet | Suhaily, Suhaimi Rohaida, Che Man Natassha, Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Aizi Nor Mazila, Ramli |
| author_sort | Suhaily, Suhaimi |
| collection | UMP |
| description | The production of cyclodextrin (CD) over the years has been increasing due to the numerous applications in industries such as in food, cosmetic, pharmaceutical and agricultural industries. However, cyclodextrin glucanotransferase (CGTase) which involved in the enzymatic reaction on the production of CD is unstable and easily denatured at extreme conditions resulted in low CD production. Hence, the enzyme immobilization technique is introduced to overcome these problems and subsequently increase the production of CD. In the present study, the CGTase was immobilized on hollow fiber membrane to increase the production of CD during the reaction. The effect of reaction conditions (types of starch, concentration of starch, temperature and pH) of the immobilized enzyme on the production of CD were investigated. Among the three types of starch tested, the soluble potato starch was the most suitable substrate for the production of CD with 4.13 mg/mL. In addition, by using 3% (w/v) of the soluble potato starch, the production of CD was 5.22 mg/mL . The optimal reaction temperature and pH were found to be at 40°C and pH 6 with 5.21 mg/mL and 4.62 mg/ml of CD, respectively. The immobilized enzyme exhibited a 1.3-3-fold increase in CD production compared to the free enzyme. Therefore, the hollow fiber membrane is suitable to be used as a support for enzyme immobilization with the high production of CD. |
| first_indexed | 2024-03-06T12:27:49Z |
| format | Article |
| id | UMPir22866 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:27:49Z |
| publishDate | 2018 |
| publisher | Penerbit Universiti Teknologi Malaysia |
| record_format | dspace |
| spelling | UMPir228662019-01-16T04:44:04Z http://umpir.ump.edu.my/id/eprint/22866/ Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme Suhaily, Suhaimi Rohaida, Che Man Natassha, Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Aizi Nor Mazila, Ramli TP Chemical technology The production of cyclodextrin (CD) over the years has been increasing due to the numerous applications in industries such as in food, cosmetic, pharmaceutical and agricultural industries. However, cyclodextrin glucanotransferase (CGTase) which involved in the enzymatic reaction on the production of CD is unstable and easily denatured at extreme conditions resulted in low CD production. Hence, the enzyme immobilization technique is introduced to overcome these problems and subsequently increase the production of CD. In the present study, the CGTase was immobilized on hollow fiber membrane to increase the production of CD during the reaction. The effect of reaction conditions (types of starch, concentration of starch, temperature and pH) of the immobilized enzyme on the production of CD were investigated. Among the three types of starch tested, the soluble potato starch was the most suitable substrate for the production of CD with 4.13 mg/mL. In addition, by using 3% (w/v) of the soluble potato starch, the production of CD was 5.22 mg/mL . The optimal reaction temperature and pH were found to be at 40°C and pH 6 with 5.21 mg/mL and 4.62 mg/ml of CD, respectively. The immobilized enzyme exhibited a 1.3-3-fold increase in CD production compared to the free enzyme. Therefore, the hollow fiber membrane is suitable to be used as a support for enzyme immobilization with the high production of CD. Penerbit Universiti Teknologi Malaysia 2018-05-01 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22866/1/Effect%20of%20reaction%20conditions%20on%20the%20synthesis%20of%20cyclodextrin%20%28CD%29%20by%20using%20immobilized%20enzyme.pdf Suhaily, Suhaimi and Rohaida, Che Man and Natassha, Jamil and Zatul Iffah, Mohd Arshad and Shalyda, Md Shaarani and Siti Zubaidah, Sulaiman and Siti Kholijah, Abdul Mudalip and Aizi Nor Mazila, Ramli (2018) Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme. Jurnal Teknologi (Sciences and Engineering), 80 (3). pp. 165-171. ISSN 0127-9696 (print); 2180-3722 (online). (Published) https://jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/11707/6370 https://doi.org/10.11113/jt.v80.11707 |
| spellingShingle | TP Chemical technology Suhaily, Suhaimi Rohaida, Che Man Natassha, Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Aizi Nor Mazila, Ramli Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme |
| title | Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme |
| title_full | Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme |
| title_fullStr | Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme |
| title_full_unstemmed | Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme |
| title_short | Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme |
| title_sort | effect of reaction conditions on the synthesis of cyclodextrin cd by using immobilized enzyme |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/22866/1/Effect%20of%20reaction%20conditions%20on%20the%20synthesis%20of%20cyclodextrin%20%28CD%29%20by%20using%20immobilized%20enzyme.pdf |
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