Solvent concentration effect on antioxidant activity and total phenolic content of Malaysian herbs

Generally, herbal plants that are relatively abundant sources of antioxidant compounds with high antioxidant activities have been consume for thousands of century by world population (Amin et al., 2004, 2006; Amin & Lee, 2005). The objective of this research was to determine total phenolic conte...

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Main Author: Nurul Atikah, Mohd Nazir
Format: Undergraduates Project Papers
Language:English
English
English
English
Published: 2016
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/23180/1/Solvent%20concentration%20effect%20on%20antioxidant%20activity%20and%20total%20phenolic%20content%20of%20Malaysian%20herbs%20-%20Table%20of%20contents.pdf
http://umpir.ump.edu.my/id/eprint/23180/2/Solvent%20concentration%20effect%20on%20antioxidant%20activity%20and%20total%20phenolic%20content%20of%20Malaysian%20herbs%20-%20Abstract.pdf
http://umpir.ump.edu.my/id/eprint/23180/3/Solvent%20concentration%20effect%20on%20antioxidant%20activity%20and%20total%20phenolic%20content%20of%20Malaysian%20herbs%20-%20Chapter%201.pdf
http://umpir.ump.edu.my/id/eprint/23180/4/Solvent%20concentration%20effect%20on%20antioxidant%20activity%20and%20total%20phenolic%20content%20of%20Malaysian%20herbs%20-%20References.pdf
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author Nurul Atikah, Mohd Nazir
author_facet Nurul Atikah, Mohd Nazir
author_sort Nurul Atikah, Mohd Nazir
collection UMP
description Generally, herbal plants that are relatively abundant sources of antioxidant compounds with high antioxidant activities have been consume for thousands of century by world population (Amin et al., 2004, 2006; Amin & Lee, 2005). The objective of this research was to determine total phenolic content, antioxidant activities of Malaysian herbal plants. The potential of using herbal Pandanus amaryllifolius (PA), Sauropus androgynous (SA) and Persicaria odorata (PO) as a natural antioxidant for food applications were investigated. Total phenolic content (TPC) of herbs was determined using Folin-Ciocalteau reagent assay and antioxidant activity were analyzed by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. The determination of TPC and antioxidant activity was done for each plant extract by using ethanol as solvent, different concentration of ethanol aqueous solutions, including 95%, 75%, 50%, 25% and 0%. Different concentration of extraction solvents significantly affected the TPC of all samples. 75% ethanol extract of Persicaria odorata (PO) exhibited the highest TPC with 6.0910 mg GAE/L extract sample. Highest antioxidant activity is also obtained by 75% ethanolic extract of PO; however all plants showed positives correlation between TPC and DPPH assay. In conclusion, all herbs exhibit the potential sources of natural antioxidant that can be used as functional food in food industry.
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spelling UMPir231802018-12-31T04:13:22Z http://umpir.ump.edu.my/id/eprint/23180/ Solvent concentration effect on antioxidant activity and total phenolic content of Malaysian herbs Nurul Atikah, Mohd Nazir TP Chemical technology Generally, herbal plants that are relatively abundant sources of antioxidant compounds with high antioxidant activities have been consume for thousands of century by world population (Amin et al., 2004, 2006; Amin & Lee, 2005). The objective of this research was to determine total phenolic content, antioxidant activities of Malaysian herbal plants. The potential of using herbal Pandanus amaryllifolius (PA), Sauropus androgynous (SA) and Persicaria odorata (PO) as a natural antioxidant for food applications were investigated. Total phenolic content (TPC) of herbs was determined using Folin-Ciocalteau reagent assay and antioxidant activity were analyzed by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. The determination of TPC and antioxidant activity was done for each plant extract by using ethanol as solvent, different concentration of ethanol aqueous solutions, including 95%, 75%, 50%, 25% and 0%. Different concentration of extraction solvents significantly affected the TPC of all samples. 75% ethanol extract of Persicaria odorata (PO) exhibited the highest TPC with 6.0910 mg GAE/L extract sample. Highest antioxidant activity is also obtained by 75% ethanolic extract of PO; however all plants showed positives correlation between TPC and DPPH assay. In conclusion, all herbs exhibit the potential sources of natural antioxidant that can be used as functional food in food industry. 2016-12 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23180/1/Solvent%20concentration%20effect%20on%20antioxidant%20activity%20and%20total%20phenolic%20content%20of%20Malaysian%20herbs%20-%20Table%20of%20contents.pdf pdf en http://umpir.ump.edu.my/id/eprint/23180/2/Solvent%20concentration%20effect%20on%20antioxidant%20activity%20and%20total%20phenolic%20content%20of%20Malaysian%20herbs%20-%20Abstract.pdf pdf en http://umpir.ump.edu.my/id/eprint/23180/3/Solvent%20concentration%20effect%20on%20antioxidant%20activity%20and%20total%20phenolic%20content%20of%20Malaysian%20herbs%20-%20Chapter%201.pdf pdf en http://umpir.ump.edu.my/id/eprint/23180/4/Solvent%20concentration%20effect%20on%20antioxidant%20activity%20and%20total%20phenolic%20content%20of%20Malaysian%20herbs%20-%20References.pdf Nurul Atikah, Mohd Nazir (2016) Solvent concentration effect on antioxidant activity and total phenolic content of Malaysian herbs. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:102958&theme=UMP2
spellingShingle TP Chemical technology
Nurul Atikah, Mohd Nazir
Solvent concentration effect on antioxidant activity and total phenolic content of Malaysian herbs
title Solvent concentration effect on antioxidant activity and total phenolic content of Malaysian herbs
title_full Solvent concentration effect on antioxidant activity and total phenolic content of Malaysian herbs
title_fullStr Solvent concentration effect on antioxidant activity and total phenolic content of Malaysian herbs
title_full_unstemmed Solvent concentration effect on antioxidant activity and total phenolic content of Malaysian herbs
title_short Solvent concentration effect on antioxidant activity and total phenolic content of Malaysian herbs
title_sort solvent concentration effect on antioxidant activity and total phenolic content of malaysian herbs
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/23180/1/Solvent%20concentration%20effect%20on%20antioxidant%20activity%20and%20total%20phenolic%20content%20of%20Malaysian%20herbs%20-%20Table%20of%20contents.pdf
http://umpir.ump.edu.my/id/eprint/23180/2/Solvent%20concentration%20effect%20on%20antioxidant%20activity%20and%20total%20phenolic%20content%20of%20Malaysian%20herbs%20-%20Abstract.pdf
http://umpir.ump.edu.my/id/eprint/23180/3/Solvent%20concentration%20effect%20on%20antioxidant%20activity%20and%20total%20phenolic%20content%20of%20Malaysian%20herbs%20-%20Chapter%201.pdf
http://umpir.ump.edu.my/id/eprint/23180/4/Solvent%20concentration%20effect%20on%20antioxidant%20activity%20and%20total%20phenolic%20content%20of%20Malaysian%20herbs%20-%20References.pdf
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