Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds
L-Asparaginase (L-asparagine amino hydrolase 3.5.1.1) constitutes one of the most biotechnologically and bio medically important group of therapeutic enzymes accounting for about 40% of the total worldwide enzyme sales [1, 2]. During current research L-asparaginase, was produced from Bacillus sp. Cu...
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| Format: | Conference or Workshop Item |
| Language: | English |
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2018
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| Online Access: | http://umpir.ump.edu.my/id/eprint/23639/1/Production%20optimization%20and%20characterization%20of%20anticancer%20enzyme%20L-Asparaginase%20from%20Bacillus%20Sp.%20using%20Moringa%20Oleifera%20Seeds.pdf |
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| author | Batool, T. Makky, Essam A. M., Khan M. M., Yusoff |
| author_facet | Batool, T. Makky, Essam A. M., Khan M. M., Yusoff |
| author_sort | Batool, T. |
| collection | UMP |
| description | L-Asparaginase (L-asparagine amino hydrolase 3.5.1.1) constitutes one of the most biotechnologically and bio medically important group of therapeutic enzymes accounting for about 40% of the total worldwide enzyme sales [1, 2]. During current research L-asparaginase, was produced from Bacillus sp. Cultural parameters affecting the production of L-asparaginase were optimized [3]. Maximal yields of L-asparaginase were recorded from 5-day-old culture grown in nutrient broth in presence of Moringa Oleifera seeds as substrate with initial pH 7.0 at temperature 37◦C [4, 5]. Ammonium chloride (1%) and galactose (1%) served as good nitrogen and carbon sources for L-asparaginase production, respectively. Na+and K+ slightly enhanced the productivity of L-asparaginase [6]. The in vitro antioxidant and antitumor activity of partially purified L-asparaginase enzyme was also studied [7]. The enzyme showed a good scavenging activity against DPPH with IC50 value of 81.645µg/mL [8]. |
| first_indexed | 2024-03-06T12:29:47Z |
| format | Conference or Workshop Item |
| id | UMPir23639 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:29:47Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | UMPir236392019-01-07T08:20:00Z http://umpir.ump.edu.my/id/eprint/23639/ Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds Batool, T. Makky, Essam A. M., Khan M. M., Yusoff Q Science (General) L-Asparaginase (L-asparagine amino hydrolase 3.5.1.1) constitutes one of the most biotechnologically and bio medically important group of therapeutic enzymes accounting for about 40% of the total worldwide enzyme sales [1, 2]. During current research L-asparaginase, was produced from Bacillus sp. Cultural parameters affecting the production of L-asparaginase were optimized [3]. Maximal yields of L-asparaginase were recorded from 5-day-old culture grown in nutrient broth in presence of Moringa Oleifera seeds as substrate with initial pH 7.0 at temperature 37◦C [4, 5]. Ammonium chloride (1%) and galactose (1%) served as good nitrogen and carbon sources for L-asparaginase production, respectively. Na+and K+ slightly enhanced the productivity of L-asparaginase [6]. The in vitro antioxidant and antitumor activity of partially purified L-asparaginase enzyme was also studied [7]. The enzyme showed a good scavenging activity against DPPH with IC50 value of 81.645µg/mL [8]. 2018 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23639/1/Production%20optimization%20and%20characterization%20of%20anticancer%20enzyme%20L-Asparaginase%20from%20Bacillus%20Sp.%20using%20Moringa%20Oleifera%20Seeds.pdf Batool, T. and Makky, Essam A. and M., Khan and M. M., Yusoff (2018) Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds. In: 4th International Conference Of Chemical Engineering And Industrial Biotechnology (ICCEIB 2018) , 1-2 Aug 2018 , Seri Pacific Hotel, Kuala Lumpur. pp. 364-365.. (Published) |
| spellingShingle | Q Science (General) Batool, T. Makky, Essam A. M., Khan M. M., Yusoff Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds |
| title | Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds |
| title_full | Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds |
| title_fullStr | Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds |
| title_full_unstemmed | Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds |
| title_short | Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds |
| title_sort | production optimization and characterization of anticancer enzyme l asparaginase from bacillus sp using moringa oleifera seeds |
| topic | Q Science (General) |
| url | http://umpir.ump.edu.my/id/eprint/23639/1/Production%20optimization%20and%20characterization%20of%20anticancer%20enzyme%20L-Asparaginase%20from%20Bacillus%20Sp.%20using%20Moringa%20Oleifera%20Seeds.pdf |
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