Fractional factorial analysis for identifying significant factors in extraction of jacalin from jackfruit seeds using anionic reverse micellar system
Reverse micellar extraction is emerging as one of the most reliable methods for extraction and purification of various proteins and enzymes. In this study, a fractional factorial design was used to identify the significant factors having the largest effects on the forward extraction process of jacal...
| Päätekijät: | , , , , |
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| Aineistotyyppi: | Conference or Workshop Item |
| Kieli: | English |
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| Linkit: | http://umpir.ump.edu.my/id/eprint/24682/1/ICCSE2018%20FULL%20PAPER%20ORAL011.pdf |
| _version_ | 1825812654431666176 |
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| author | Sharifah Fathiyah, Sy Mohamad Farhan, Mohd Said Mimi Sakinah, A. M. Shahril, Mohamad Wan Azizi, Wan Sulaiman |
| author_facet | Sharifah Fathiyah, Sy Mohamad Farhan, Mohd Said Mimi Sakinah, A. M. Shahril, Mohamad Wan Azizi, Wan Sulaiman |
| author_sort | Sharifah Fathiyah, Sy Mohamad |
| collection | UMP |
| description | Reverse micellar extraction is emerging as one of the most reliable methods for extraction and purification of various proteins and enzymes. In this study, a fractional factorial design was used to identify the significant factors having the largest effects on the forward extraction process of jacalin using reverse micelles formed by an anionic surfactant, sodium bis(2-ethylhexyl) sulfosuccinate (AOT). Effect of five different factors, namely the aqueous phase pH, surfactant (AOT) concentration, salt (NaCl) concentration, contact time and the volume ratio of organic to aqueous phase on the forward extraction efficiency were investigated using the 25-1 fractional factorial design. The results showed that only the aqueous phase pH, NaCl concentration and concentration of AOT had significant effects on the forward extraction efficiency of jacalin into AOT reverse micelles. Results obtained indicated that the fractional factorial design was a useful method for screening many process factors with a minimum number of experiments. |
| first_indexed | 2024-03-06T12:32:22Z |
| format | Conference or Workshop Item |
| id | UMPir24682 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:32:22Z |
| record_format | dspace |
| spelling | UMPir246822019-06-10T07:44:10Z http://umpir.ump.edu.my/id/eprint/24682/ Fractional factorial analysis for identifying significant factors in extraction of jacalin from jackfruit seeds using anionic reverse micellar system Sharifah Fathiyah, Sy Mohamad Farhan, Mohd Said Mimi Sakinah, A. M. Shahril, Mohamad Wan Azizi, Wan Sulaiman T Technology (General) TP Chemical technology Reverse micellar extraction is emerging as one of the most reliable methods for extraction and purification of various proteins and enzymes. In this study, a fractional factorial design was used to identify the significant factors having the largest effects on the forward extraction process of jacalin using reverse micelles formed by an anionic surfactant, sodium bis(2-ethylhexyl) sulfosuccinate (AOT). Effect of five different factors, namely the aqueous phase pH, surfactant (AOT) concentration, salt (NaCl) concentration, contact time and the volume ratio of organic to aqueous phase on the forward extraction efficiency were investigated using the 25-1 fractional factorial design. The results showed that only the aqueous phase pH, NaCl concentration and concentration of AOT had significant effects on the forward extraction efficiency of jacalin into AOT reverse micelles. Results obtained indicated that the fractional factorial design was a useful method for screening many process factors with a minimum number of experiments. Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/24682/1/ICCSE2018%20FULL%20PAPER%20ORAL011.pdf Sharifah Fathiyah, Sy Mohamad and Farhan, Mohd Said and Mimi Sakinah, A. M. and Shahril, Mohamad and Wan Azizi, Wan Sulaiman Fractional factorial analysis for identifying significant factors in extraction of jacalin from jackfruit seeds using anionic reverse micellar system. In: International Conference on Chemical Sciences and Engineering: Advance and New Materials (ICCSE 2018) , 7 - 8 November 2018 , Ramada Plaza, Melaka. pp. 1-9.. (Submitted) (Submitted) |
| spellingShingle | T Technology (General) TP Chemical technology Sharifah Fathiyah, Sy Mohamad Farhan, Mohd Said Mimi Sakinah, A. M. Shahril, Mohamad Wan Azizi, Wan Sulaiman Fractional factorial analysis for identifying significant factors in extraction of jacalin from jackfruit seeds using anionic reverse micellar system |
| title | Fractional factorial analysis for identifying significant factors in extraction of jacalin from jackfruit seeds using anionic reverse micellar system |
| title_full | Fractional factorial analysis for identifying significant factors in extraction of jacalin from jackfruit seeds using anionic reverse micellar system |
| title_fullStr | Fractional factorial analysis for identifying significant factors in extraction of jacalin from jackfruit seeds using anionic reverse micellar system |
| title_full_unstemmed | Fractional factorial analysis for identifying significant factors in extraction of jacalin from jackfruit seeds using anionic reverse micellar system |
| title_short | Fractional factorial analysis for identifying significant factors in extraction of jacalin from jackfruit seeds using anionic reverse micellar system |
| title_sort | fractional factorial analysis for identifying significant factors in extraction of jacalin from jackfruit seeds using anionic reverse micellar system |
| topic | T Technology (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/24682/1/ICCSE2018%20FULL%20PAPER%20ORAL011.pdf |
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