Optimization of xylose production from sugarcane bagasse using response surface methodology (RSM)

Xylose is a monosaccharide containing five carbon atoms, including an aldehyde functional group. It is a pentose sugar which has chemical formula C5H10O5. Hemicellulose is present in plant cell walls and is associated with the cellulose. Its chemical formula is (C5H8O4)n and in some cases is (C6H10O...

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Main Author: Nurul Izwanie, Rasli
Format: Undergraduates Project Papers
Language:English
Published: 2010
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/2482/1/Optimization%20of%20xylose%20production%20from%20sugarcane%20bagasse%20using%20response%20surface%20methodology%20%28RSM%29.pdf
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author Nurul Izwanie, Rasli
author_facet Nurul Izwanie, Rasli
author_sort Nurul Izwanie, Rasli
collection UMP
description Xylose is a monosaccharide containing five carbon atoms, including an aldehyde functional group. It is a pentose sugar which has chemical formula C5H10O5. Hemicellulose is present in plant cell walls and is associated with the cellulose. Its chemical formula is (C5H8O4)n and in some cases is (C6H10O5)n. It is possible to hydrolyze hemicellulose by several processes (enzymatic, physical and chemical) for producing monomer sugars with great purity and high yield. The aim of this study is to optimize the xylose production from sugarcane bagasse by manipulating the temperature, agitation rate and enzyme concentration using Response Surface Methodology (RSM) based on central composite design (CCD). In this study, producing xylose from sugarcane bagasse contributes to reduce the environmental impact and bioprocess cost. Alkaline and acid hydrolysis method was used for the pretreatment of sugarcane bagasse. After the pretreatment, the screening process was constructed to determine the best range of parameters to be used in optimization process. Seventeen experiments have been arranged by RSM for optimization. The optimized conditions of parameters were 50°C of temperature, 180 rpm of agitation rate and 2 mg/ml of enzyme concentration with the predicted xylose production was 0.367 mg/ml. The actual xylose production was 0.373 mg/ml. Before the optimization, the xylose production was 0.228 mg/ml. As a conclusion, the optimization of xylose production from sugarcane bagasse by using RSM was successfully done with 63.6% of increment.
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spelling UMPir24822023-10-19T07:45:20Z http://umpir.ump.edu.my/id/eprint/2482/ Optimization of xylose production from sugarcane bagasse using response surface methodology (RSM) Nurul Izwanie, Rasli TP Chemical technology Xylose is a monosaccharide containing five carbon atoms, including an aldehyde functional group. It is a pentose sugar which has chemical formula C5H10O5. Hemicellulose is present in plant cell walls and is associated with the cellulose. Its chemical formula is (C5H8O4)n and in some cases is (C6H10O5)n. It is possible to hydrolyze hemicellulose by several processes (enzymatic, physical and chemical) for producing monomer sugars with great purity and high yield. The aim of this study is to optimize the xylose production from sugarcane bagasse by manipulating the temperature, agitation rate and enzyme concentration using Response Surface Methodology (RSM) based on central composite design (CCD). In this study, producing xylose from sugarcane bagasse contributes to reduce the environmental impact and bioprocess cost. Alkaline and acid hydrolysis method was used for the pretreatment of sugarcane bagasse. After the pretreatment, the screening process was constructed to determine the best range of parameters to be used in optimization process. Seventeen experiments have been arranged by RSM for optimization. The optimized conditions of parameters were 50°C of temperature, 180 rpm of agitation rate and 2 mg/ml of enzyme concentration with the predicted xylose production was 0.367 mg/ml. The actual xylose production was 0.373 mg/ml. Before the optimization, the xylose production was 0.228 mg/ml. As a conclusion, the optimization of xylose production from sugarcane bagasse by using RSM was successfully done with 63.6% of increment. 2010-12 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/2482/1/Optimization%20of%20xylose%20production%20from%20sugarcane%20bagasse%20using%20response%20surface%20methodology%20%28RSM%29.pdf Nurul Izwanie, Rasli (2010) Optimization of xylose production from sugarcane bagasse using response surface methodology (RSM). Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
spellingShingle TP Chemical technology
Nurul Izwanie, Rasli
Optimization of xylose production from sugarcane bagasse using response surface methodology (RSM)
title Optimization of xylose production from sugarcane bagasse using response surface methodology (RSM)
title_full Optimization of xylose production from sugarcane bagasse using response surface methodology (RSM)
title_fullStr Optimization of xylose production from sugarcane bagasse using response surface methodology (RSM)
title_full_unstemmed Optimization of xylose production from sugarcane bagasse using response surface methodology (RSM)
title_short Optimization of xylose production from sugarcane bagasse using response surface methodology (RSM)
title_sort optimization of xylose production from sugarcane bagasse using response surface methodology rsm
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/2482/1/Optimization%20of%20xylose%20production%20from%20sugarcane%20bagasse%20using%20response%20surface%20methodology%20%28RSM%29.pdf
work_keys_str_mv AT nurulizwanierasli optimizationofxyloseproductionfromsugarcanebagasseusingresponsesurfacemethodologyrsm