Antiangiogenic activity of sophorolipids extracted from refined bleached deodorized palm olein
OBJECTIVES: Sophorolipids (SLs) are a group of surface-active glycolipids produced by a type of nonpathogenic yeast Candida bombicola in the presence of vegetable oil through fermentation technology. SLs have shown antitumor activity; however, the mechanism of action underlying the anticancer activi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/24986/1/Antiangiogenic%20activity%20of%20sophorolipids%20extracted%20from%20refined%20bleached.pdf |
| _version_ | 1825812709809061888 |
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| author | Mohamed, Shazmin Kithur Asif, Muhammad Nazari, Mansoureh Vishkaei Baharetha, Hussein M. Mahmood, Syed Abdul Rashid, M. Yatim Aman Shah, Abdul Majid Abdul Majid, Amin Malik Shah |
| author_facet | Mohamed, Shazmin Kithur Asif, Muhammad Nazari, Mansoureh Vishkaei Baharetha, Hussein M. Mahmood, Syed Abdul Rashid, M. Yatim Aman Shah, Abdul Majid Abdul Majid, Amin Malik Shah |
| author_sort | Mohamed, Shazmin Kithur |
| collection | UMP |
| description | OBJECTIVES: Sophorolipids (SLs) are a group of surface-active glycolipids produced by a type of nonpathogenic yeast Candida bombicola in the presence of vegetable oil through fermentation technology. SLs have shown antitumor activity; however, the mechanism of action underlying the anticancer activity of SLs is poorly understood. This work evaluated the anticancer activity of SLs fermented from palm oil by exploring its antiangiogenic activity. MATERIALS AND METHODS: The SLs that were fermented and further characterized for their biochemical activities. Cytotoxicity study was performed to assess cytostatic properties. A series of in vitro and ex vivo angiogenesis assay was also carried out. The relative fold change in the expression of p53 mRNA by SLs was also studied. RESULTS: Altogether, the data show that SLs derived from palm oil fermentation process inhibited neovascularization in the ex vivo tissue segments and also the endothelial cell proliferation between 50% and 65% inhibition as a whole. The palm oil derived SLs also caused downregulation of the suppression level of vascular endothelial growth factor and also upregulate the p53 mRNA level. The analytical studies revealed the presence of high amount of phenolic compounds but with relatively weak antioxidant activity. The gas chromatography-mass spectrometry studies revealed abundant amount of palmitic and oleic acid, the latter an established antiangiogenic agent, and the former being proangiogenic. CONCLUSION: Therefore, it can be concluded from this study that SLs derived from fermented palm oil have potent antiangiogenic activity which may be attributed by its oleic acid component. |
| first_indexed | 2024-03-06T12:33:10Z |
| format | Article |
| id | UMPir24986 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:33:10Z |
| publishDate | 2019 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | dspace |
| spelling | UMPir249862019-05-30T08:29:02Z http://umpir.ump.edu.my/id/eprint/24986/ Antiangiogenic activity of sophorolipids extracted from refined bleached deodorized palm olein Mohamed, Shazmin Kithur Asif, Muhammad Nazari, Mansoureh Vishkaei Baharetha, Hussein M. Mahmood, Syed Abdul Rashid, M. Yatim Aman Shah, Abdul Majid Abdul Majid, Amin Malik Shah QD Chemistry QH Natural history TP Chemical technology OBJECTIVES: Sophorolipids (SLs) are a group of surface-active glycolipids produced by a type of nonpathogenic yeast Candida bombicola in the presence of vegetable oil through fermentation technology. SLs have shown antitumor activity; however, the mechanism of action underlying the anticancer activity of SLs is poorly understood. This work evaluated the anticancer activity of SLs fermented from palm oil by exploring its antiangiogenic activity. MATERIALS AND METHODS: The SLs that were fermented and further characterized for their biochemical activities. Cytotoxicity study was performed to assess cytostatic properties. A series of in vitro and ex vivo angiogenesis assay was also carried out. The relative fold change in the expression of p53 mRNA by SLs was also studied. RESULTS: Altogether, the data show that SLs derived from palm oil fermentation process inhibited neovascularization in the ex vivo tissue segments and also the endothelial cell proliferation between 50% and 65% inhibition as a whole. The palm oil derived SLs also caused downregulation of the suppression level of vascular endothelial growth factor and also upregulate the p53 mRNA level. The analytical studies revealed the presence of high amount of phenolic compounds but with relatively weak antioxidant activity. The gas chromatography-mass spectrometry studies revealed abundant amount of palmitic and oleic acid, the latter an established antiangiogenic agent, and the former being proangiogenic. CONCLUSION: Therefore, it can be concluded from this study that SLs derived from fermented palm oil have potent antiangiogenic activity which may be attributed by its oleic acid component. Wolters Kluwer Medknow Publications 2019-01 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/24986/1/Antiangiogenic%20activity%20of%20sophorolipids%20extracted%20from%20refined%20bleached.pdf Mohamed, Shazmin Kithur and Asif, Muhammad and Nazari, Mansoureh Vishkaei and Baharetha, Hussein M. and Mahmood, Syed and Abdul Rashid, M. Yatim and Aman Shah, Abdul Majid and Abdul Majid, Amin Malik Shah (2019) Antiangiogenic activity of sophorolipids extracted from refined bleached deodorized palm olein. Indian Journal of Pharmacology, 51 (1). pp. 45-54. ISSN 0253-7613. (Published) https://doi.org/10.4103/ijp.IJP_312_18 https://doi.org/10.4103/ijp.IJP_312_18 |
| spellingShingle | QD Chemistry QH Natural history TP Chemical technology Mohamed, Shazmin Kithur Asif, Muhammad Nazari, Mansoureh Vishkaei Baharetha, Hussein M. Mahmood, Syed Abdul Rashid, M. Yatim Aman Shah, Abdul Majid Abdul Majid, Amin Malik Shah Antiangiogenic activity of sophorolipids extracted from refined bleached deodorized palm olein |
| title | Antiangiogenic activity of sophorolipids extracted from refined bleached deodorized palm olein |
| title_full | Antiangiogenic activity of sophorolipids extracted from refined bleached deodorized palm olein |
| title_fullStr | Antiangiogenic activity of sophorolipids extracted from refined bleached deodorized palm olein |
| title_full_unstemmed | Antiangiogenic activity of sophorolipids extracted from refined bleached deodorized palm olein |
| title_short | Antiangiogenic activity of sophorolipids extracted from refined bleached deodorized palm olein |
| title_sort | antiangiogenic activity of sophorolipids extracted from refined bleached deodorized palm olein |
| topic | QD Chemistry QH Natural history TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/24986/1/Antiangiogenic%20activity%20of%20sophorolipids%20extracted%20from%20refined%20bleached.pdf |
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