Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine
Keratin was extracted from different segments of disposable waste chicken feathers (CF) including the whole feathers, calamus/rachis (β-sheet) and barbs/barbules (α-helix), using sodium sulfide and L-cysteine. The yield of extracted keratin from sodium sulfide and L-cysteine was ˜88% and ˜66% respec...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier Ltd
2019
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| Online Access: | http://umpir.ump.edu.my/id/eprint/25071/1/Extraction%20of%20keratin%20from%20waste%20chicken%20feathers%20using%20.pdf |
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| author | Pourjavaheri, Firoozeh Ostovar Pour, Saeideh Jones, Oliver A. H. Smooker, Peter M. Brkljača, Robert Sherkat, Frank Blanch, Ewan W. Gupta, Arun Shanks, Robert A. |
| author_facet | Pourjavaheri, Firoozeh Ostovar Pour, Saeideh Jones, Oliver A. H. Smooker, Peter M. Brkljača, Robert Sherkat, Frank Blanch, Ewan W. Gupta, Arun Shanks, Robert A. |
| author_sort | Pourjavaheri, Firoozeh |
| collection | UMP |
| description | Keratin was extracted from different segments of disposable waste chicken feathers (CF) including the whole feathers, calamus/rachis (β-sheet) and barbs/barbules (α-helix), using sodium sulfide and L-cysteine. The yield of extracted keratin from sodium sulfide and L-cysteine was ˜88% and ˜66% respectively. The mass ratio of feathers to reducing agent was 1:20 and the reaction temperature was 40 °C for 6 h. Concentration of keratin extracted by each method was measured using the Bradford assay. The protein extracted from each feather section was characterised using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, vibrational spectroscopy including FTIR and Raman, nuclear magnetic resonance, and thermogravimetry. These results confirmed the keratin structures after each extraction methods. The study showed that α-helix and ß-sheet based keratin could be extracted from CF using sodium sulfide and L-cysteine with high yields. This is the first report of CF keratin extraction using L-cysteine. |
| first_indexed | 2024-03-06T12:33:25Z |
| format | Article |
| id | UMPir25071 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:33:25Z |
| publishDate | 2019 |
| publisher | Elsevier Ltd |
| record_format | dspace |
| spelling | UMPir250712019-10-24T06:41:14Z http://umpir.ump.edu.my/id/eprint/25071/ Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine Pourjavaheri, Firoozeh Ostovar Pour, Saeideh Jones, Oliver A. H. Smooker, Peter M. Brkljača, Robert Sherkat, Frank Blanch, Ewan W. Gupta, Arun Shanks, Robert A. QD Chemistry TP Chemical technology Keratin was extracted from different segments of disposable waste chicken feathers (CF) including the whole feathers, calamus/rachis (β-sheet) and barbs/barbules (α-helix), using sodium sulfide and L-cysteine. The yield of extracted keratin from sodium sulfide and L-cysteine was ˜88% and ˜66% respectively. The mass ratio of feathers to reducing agent was 1:20 and the reaction temperature was 40 °C for 6 h. Concentration of keratin extracted by each method was measured using the Bradford assay. The protein extracted from each feather section was characterised using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, vibrational spectroscopy including FTIR and Raman, nuclear magnetic resonance, and thermogravimetry. These results confirmed the keratin structures after each extraction methods. The study showed that α-helix and ß-sheet based keratin could be extracted from CF using sodium sulfide and L-cysteine with high yields. This is the first report of CF keratin extraction using L-cysteine. Elsevier Ltd 2019-07 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25071/1/Extraction%20of%20keratin%20from%20waste%20chicken%20feathers%20using%20.pdf Pourjavaheri, Firoozeh and Ostovar Pour, Saeideh and Jones, Oliver A. H. and Smooker, Peter M. and Brkljača, Robert and Sherkat, Frank and Blanch, Ewan W. and Gupta, Arun and Shanks, Robert A. (2019) Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine. Process Biochemistry, 82. pp. 205-214. ISSN 1359-5113. (Published) https://doi.org/10.1016/j.procbio.2019.04.010 https://doi.org/10.1016/j.procbio.2019.04.010 |
| spellingShingle | QD Chemistry TP Chemical technology Pourjavaheri, Firoozeh Ostovar Pour, Saeideh Jones, Oliver A. H. Smooker, Peter M. Brkljača, Robert Sherkat, Frank Blanch, Ewan W. Gupta, Arun Shanks, Robert A. Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine |
| title | Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine |
| title_full | Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine |
| title_fullStr | Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine |
| title_full_unstemmed | Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine |
| title_short | Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine |
| title_sort | extraction of keratin from waste chicken feathers using sodium sulfide and l cysteine |
| topic | QD Chemistry TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/25071/1/Extraction%20of%20keratin%20from%20waste%20chicken%20feathers%20using%20.pdf |
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