Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles
This study demonstrates the extraction of jacalin from crude extract of jackfruit seeds using anionic surfactant based reverse micellar system, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. Effects of various parameters, such as, NaCl concentration (0.05 – 1 M), pH of aqueous phase (pH...
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| Format: | Article |
| Language: | English |
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Department of Chemistry, Universitas Gadjah Mada Yogyakarta
2019
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| Online Access: | http://umpir.ump.edu.my/id/eprint/25736/1/Effect%20of%20key%20parameters%20on%20jacalin%20extraction.pdf |
| _version_ | 1825812849592631296 |
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| author | Sharifah Fathiyah, Sy Mohamad Farhan, Mohd Said Mimi Sakinah, A. M. Shahril, Mohamad |
| author_facet | Sharifah Fathiyah, Sy Mohamad Farhan, Mohd Said Mimi Sakinah, A. M. Shahril, Mohamad |
| author_sort | Sharifah Fathiyah, Sy Mohamad |
| collection | UMP |
| description | This study demonstrates the extraction of jacalin from crude extract of jackfruit seeds using anionic surfactant based reverse micellar system, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. Effects of various parameters, such as, NaCl concentration (0.05 – 1 M), pH of aqueous phase (pH 4 – 10), AOT concentration (5 – 150 mM), contact time (5 – 30 min) and phase volume ratio (0.5-5) on the transfer efficiency of jacalin was evaluated by changing one factor at a time (OFAT) while keeping the other parameters constant. A maximum of 83% of protein transfer was achieved after equal volume of organic and aqueous phase was stirred for 20 min using 20 mM AOT at pH 5 aqueous phase containing 0.1 M NaCl. The findings demonstrated AOT reverse micellar system as a promising and effective method to extract and purify jacalin from crude protein mixture. |
| first_indexed | 2024-03-06T12:35:15Z |
| format | Article |
| id | UMPir25736 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:35:15Z |
| publishDate | 2019 |
| publisher | Department of Chemistry, Universitas Gadjah Mada Yogyakarta |
| record_format | dspace |
| spelling | UMPir257362019-11-22T04:09:05Z http://umpir.ump.edu.my/id/eprint/25736/ Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles Sharifah Fathiyah, Sy Mohamad Farhan, Mohd Said Mimi Sakinah, A. M. Shahril, Mohamad TP Chemical technology This study demonstrates the extraction of jacalin from crude extract of jackfruit seeds using anionic surfactant based reverse micellar system, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. Effects of various parameters, such as, NaCl concentration (0.05 – 1 M), pH of aqueous phase (pH 4 – 10), AOT concentration (5 – 150 mM), contact time (5 – 30 min) and phase volume ratio (0.5-5) on the transfer efficiency of jacalin was evaluated by changing one factor at a time (OFAT) while keeping the other parameters constant. A maximum of 83% of protein transfer was achieved after equal volume of organic and aqueous phase was stirred for 20 min using 20 mM AOT at pH 5 aqueous phase containing 0.1 M NaCl. The findings demonstrated AOT reverse micellar system as a promising and effective method to extract and purify jacalin from crude protein mixture. Department of Chemistry, Universitas Gadjah Mada Yogyakarta 2019-08 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/25736/1/Effect%20of%20key%20parameters%20on%20jacalin%20extraction.pdf Sharifah Fathiyah, Sy Mohamad and Farhan, Mohd Said and Mimi Sakinah, A. M. and Shahril, Mohamad (2019) Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles. Indonesian Journal of Chemistry, 19 (3). pp. 737-746. ISSN 1411-9420 (Print); 2460-1578 (Online). (Published) https://doi.org/10.22146/ijc.39705 https://doi.org/10.22146/ijc.39705 |
| spellingShingle | TP Chemical technology Sharifah Fathiyah, Sy Mohamad Farhan, Mohd Said Mimi Sakinah, A. M. Shahril, Mohamad Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles |
| title | Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles |
| title_full | Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles |
| title_fullStr | Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles |
| title_full_unstemmed | Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles |
| title_short | Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles |
| title_sort | effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/25736/1/Effect%20of%20key%20parameters%20on%20jacalin%20extraction.pdf |
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