Optimization of process parameters for bioethanol production from oil palm frond juice by Saccharomyces cerevisiae using response surface methodology as a tool

The aim of this research was to investigate the effect of temperature, medium initial pH and rotation rate on the production of bioethanol from OPF juice without nutrient and nitrogen source supplementation using Saccharomyces cerevisiae Kyokai No. 7 (ATCC 26622). A five-level-three-factor central c...

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Main Authors: Siti Hajar, Mat Zani, Faatihah, Mohamad Asri, Nina Suhaity, Azmi, Hafizuddin, Wan Yussof, Mior Ahmad Khushairi, Mohd Zahari
Format: Conference or Workshop Item
Language:English
Published: IOP Publishing 2019
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/25817/1/Optimization%20of%20process%20parameters%20for%20bioethanol%20production.pdf
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author Siti Hajar, Mat Zani
Faatihah, Mohamad Asri
Nina Suhaity, Azmi
Hafizuddin, Wan Yussof
Mior Ahmad Khushairi, Mohd Zahari
author_facet Siti Hajar, Mat Zani
Faatihah, Mohamad Asri
Nina Suhaity, Azmi
Hafizuddin, Wan Yussof
Mior Ahmad Khushairi, Mohd Zahari
author_sort Siti Hajar, Mat Zani
collection UMP
description The aim of this research was to investigate the effect of temperature, medium initial pH and rotation rate on the production of bioethanol from OPF juice without nutrient and nitrogen source supplementation using Saccharomyces cerevisiae Kyokai No. 7 (ATCC 26622). A five-level-three-factor central composite design (CCD) was employed in this study and the central point of each process variable was chosen based on the best condition obtained from the one-factor-at-time (OFAT) method. The parameters ranges were set as follows; medium initial pH (5-9), temperature (27.5-37.5°C) and rotation rate (80-120 rpm). Bioethanol and residual sugars concentration were determine using High Performance Liquid Chromatography (HPLC). The optimum conditions for bioethanol production from OPF juice were achieved at medium initial pH (6.62), rotation rate (96.51 rpm) and temperature (33.03°C). Based on the validation experiment, the optimum bioethanol yield was 0.50 ±0.02 g/g sugars and this value was in close agreement with the model prediction where the difference was only 4.17%. Under the optimal conditions, the bioethanol yield obtained was 47.06% higher compared with non-optimized condition. The promising yield obtained in this study suggests that OPF juice can be used as a renewable and complete fermentation feedstock for bioethanol production.
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spelling UMPir258172023-08-08T08:37:52Z http://umpir.ump.edu.my/id/eprint/25817/ Optimization of process parameters for bioethanol production from oil palm frond juice by Saccharomyces cerevisiae using response surface methodology as a tool Siti Hajar, Mat Zani Faatihah, Mohamad Asri Nina Suhaity, Azmi Hafizuddin, Wan Yussof Mior Ahmad Khushairi, Mohd Zahari TP Chemical technology The aim of this research was to investigate the effect of temperature, medium initial pH and rotation rate on the production of bioethanol from OPF juice without nutrient and nitrogen source supplementation using Saccharomyces cerevisiae Kyokai No. 7 (ATCC 26622). A five-level-three-factor central composite design (CCD) was employed in this study and the central point of each process variable was chosen based on the best condition obtained from the one-factor-at-time (OFAT) method. The parameters ranges were set as follows; medium initial pH (5-9), temperature (27.5-37.5°C) and rotation rate (80-120 rpm). Bioethanol and residual sugars concentration were determine using High Performance Liquid Chromatography (HPLC). The optimum conditions for bioethanol production from OPF juice were achieved at medium initial pH (6.62), rotation rate (96.51 rpm) and temperature (33.03°C). Based on the validation experiment, the optimum bioethanol yield was 0.50 ±0.02 g/g sugars and this value was in close agreement with the model prediction where the difference was only 4.17%. Under the optimal conditions, the bioethanol yield obtained was 47.06% higher compared with non-optimized condition. The promising yield obtained in this study suggests that OPF juice can be used as a renewable and complete fermentation feedstock for bioethanol production. IOP Publishing 2019-09-04 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/25817/1/Optimization%20of%20process%20parameters%20for%20bioethanol%20production.pdf Siti Hajar, Mat Zani and Faatihah, Mohamad Asri and Nina Suhaity, Azmi and Hafizuddin, Wan Yussof and Mior Ahmad Khushairi, Mohd Zahari (2019) Optimization of process parameters for bioethanol production from oil palm frond juice by Saccharomyces cerevisiae using response surface methodology as a tool. In: IOP Conference Series: Materials Science and Engineering, 1st ProSES Symposium 2019 , 4 September 2019 , Kuantan, Pahang, Malaysia. pp. 1-15., 702 (012003). ISSN 1757-899X https://doi.org/10.1088/1757-899X/702/1/012003
spellingShingle TP Chemical technology
Siti Hajar, Mat Zani
Faatihah, Mohamad Asri
Nina Suhaity, Azmi
Hafizuddin, Wan Yussof
Mior Ahmad Khushairi, Mohd Zahari
Optimization of process parameters for bioethanol production from oil palm frond juice by Saccharomyces cerevisiae using response surface methodology as a tool
title Optimization of process parameters for bioethanol production from oil palm frond juice by Saccharomyces cerevisiae using response surface methodology as a tool
title_full Optimization of process parameters for bioethanol production from oil palm frond juice by Saccharomyces cerevisiae using response surface methodology as a tool
title_fullStr Optimization of process parameters for bioethanol production from oil palm frond juice by Saccharomyces cerevisiae using response surface methodology as a tool
title_full_unstemmed Optimization of process parameters for bioethanol production from oil palm frond juice by Saccharomyces cerevisiae using response surface methodology as a tool
title_short Optimization of process parameters for bioethanol production from oil palm frond juice by Saccharomyces cerevisiae using response surface methodology as a tool
title_sort optimization of process parameters for bioethanol production from oil palm frond juice by saccharomyces cerevisiae using response surface methodology as a tool
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/25817/1/Optimization%20of%20process%20parameters%20for%20bioethanol%20production.pdf
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