Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3)
ᴅ-xylonic acid is a five-carbon organic acid that has gain increasing interest due to its broadly flexible application in fields such as foods, pharmaceutical and agriculture. Microbial production of ᴅ-xylonic acid can be done by using many species of Pseudomonas, Acetobacter, Gluconobacter and the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier Ltd
2019
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/25949/1/effect%20of%20pyhsical%20parameters.pdf |
| _version_ | 1825812886401843200 |
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| author | Nurul Atikah, Mohd Rodzri Salwani, Md Zain Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah R. M., Illias |
| author_facet | Nurul Atikah, Mohd Rodzri Salwani, Md Zain Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah R. M., Illias |
| author_sort | Nurul Atikah, Mohd Rodzri |
| collection | UMP |
| description | ᴅ-xylonic acid is a five-carbon organic acid that has gain increasing interest due to its broadly flexible application in fields such as foods, pharmaceutical and agriculture. Microbial production of ᴅ-xylonic acid can be done by using many species of Pseudomonas, Acetobacter, Gluconobacter and the latest finding was by engineered microorganism. In this study, recombinant E. coli BL21 (DE3) is used to produce ᴅ-xylonic acid from ᴅ-xylose and the effect of temperature, initial pH of medium and agitation rate on ᴅ-xylonic acid production is determined. Fermentation was carried out using super optimal broth medium in shake flasks for 24 hours. Results indicate that ᴅ-xylonic acid concentration was as high as 9.95 g L-1 with yield of 0.91 g/g ᴅ-xylose from 10 g/L when governed under temperature of 37°C, initial pH of medium pH 7 and agitation rate 200 rpm. |
| first_indexed | 2024-03-06T12:35:47Z |
| format | Article |
| id | UMPir25949 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:35:47Z |
| publishDate | 2019 |
| publisher | Elsevier Ltd |
| record_format | dspace |
| spelling | UMPir259492021-02-09T08:45:24Z http://umpir.ump.edu.my/id/eprint/25949/ Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3) Nurul Atikah, Mohd Rodzri Salwani, Md Zain Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah R. M., Illias TP Chemical technology ᴅ-xylonic acid is a five-carbon organic acid that has gain increasing interest due to its broadly flexible application in fields such as foods, pharmaceutical and agriculture. Microbial production of ᴅ-xylonic acid can be done by using many species of Pseudomonas, Acetobacter, Gluconobacter and the latest finding was by engineered microorganism. In this study, recombinant E. coli BL21 (DE3) is used to produce ᴅ-xylonic acid from ᴅ-xylose and the effect of temperature, initial pH of medium and agitation rate on ᴅ-xylonic acid production is determined. Fermentation was carried out using super optimal broth medium in shake flasks for 24 hours. Results indicate that ᴅ-xylonic acid concentration was as high as 9.95 g L-1 with yield of 0.91 g/g ᴅ-xylose from 10 g/L when governed under temperature of 37°C, initial pH of medium pH 7 and agitation rate 200 rpm. Elsevier Ltd 2019 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25949/1/effect%20of%20pyhsical%20parameters.pdf Nurul Atikah, Mohd Rodzri and Salwani, Md Zain and Rafidah, Mohamed Ali Hanapiah and Rozaimi, Abu Samah and R. M., Illias (2019) Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3). Materials Today: Proceedings, 19 (4). pp. 1247-1254. ISSN 2214-7853. (Published) https://doi.org/10.1016/j.matpr.2019.11.129 https://doi.org/10.1016/j.matpr.2019.11.129 |
| spellingShingle | TP Chemical technology Nurul Atikah, Mohd Rodzri Salwani, Md Zain Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah R. M., Illias Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3) |
| title | Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3) |
| title_full | Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3) |
| title_fullStr | Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3) |
| title_full_unstemmed | Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3) |
| title_short | Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3) |
| title_sort | effect of physical parameters on d xylonic acid production using recombinant e coli bl21 de3 |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/25949/1/effect%20of%20pyhsical%20parameters.pdf |
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