Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool
Fenugreek is a multiregional plant mostly found in Asia, the Middle East, and some European countries. Thus, this study aimed to optimize the microwave-assisted extraction (MAE) process parameters such as irradiation time (2–4 min), ethanol concentration (40–80%), microwave power (500–700 W), solid-...
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| Format: | Article |
| Language: | English |
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Elsevier Masson SAS
2019
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| Online Access: | http://umpir.ump.edu.my/id/eprint/26647/1/Optimization%20of%20saponins1.pdf |
| _version_ | 1825813016813240320 |
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| author | Akbari, Sweeta Nour, A. H. R. M., Yunus |
| author_facet | Akbari, Sweeta Nour, A. H. R. M., Yunus |
| author_sort | Akbari, Sweeta |
| collection | UMP |
| description | Fenugreek is a multiregional plant mostly found in Asia, the Middle East, and some European countries. Thus, this study aimed to optimize the microwave-assisted extraction (MAE) process parameters such as irradiation time (2–4 min), ethanol concentration (40–80%), microwave power (500–700 W), solid-to-liquid ratio (1:8–1:12 g/mL), and a fixed extraction temperature (70 °C) for fenugreek seed powder. Response surface methodology (RSM) was applied to optimize MAE process parameters to achieve optimal total saponin content (TSC), total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) antioxidant capacities of fenugreek seed extract. The levels of independent variables were first evaluated using one-factor-at-one-time method, after which the suitable levels (−1, 0, and +1) were selected for the optimization process. The optimal conditions of MAE parameters were found to be 2.84 min, 572.50 W, 63.68%, and 0.09 g/mL. Based on the suggested optimum conditions, the obtained responses were 195.89 ± 1.07 (mg diosgenin equivalent/g d.w.), 81.85 ± 0.61 (mg gallic acid equivalent/g d.w.), 92.86 ± 0.56%, and 95.85 ± 0.81%. Analysis of variance revealed that all the responses including TSC, TPC, DPPH, and ABTS antioxidant capacities were significantly influenced (P < 0.05) by irradiation time, solvent concentration, microwave oven power, and feed-to-solvent ratio. Hence, the results obtained from liquid chromatography–quadrupole–time-of-flight mass spectrometry indicated the presence of steroid saponins, triterpenoid saponins, phenolics, and other natural antioxidants. |
| first_indexed | 2024-03-06T12:37:45Z |
| format | Article |
| id | UMPir26647 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:37:45Z |
| publishDate | 2019 |
| publisher | Elsevier Masson SAS |
| record_format | dspace |
| spelling | UMPir266472019-11-28T04:43:06Z http://umpir.ump.edu.my/id/eprint/26647/ Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool Akbari, Sweeta Nour, A. H. R. M., Yunus TP Chemical technology Fenugreek is a multiregional plant mostly found in Asia, the Middle East, and some European countries. Thus, this study aimed to optimize the microwave-assisted extraction (MAE) process parameters such as irradiation time (2–4 min), ethanol concentration (40–80%), microwave power (500–700 W), solid-to-liquid ratio (1:8–1:12 g/mL), and a fixed extraction temperature (70 °C) for fenugreek seed powder. Response surface methodology (RSM) was applied to optimize MAE process parameters to achieve optimal total saponin content (TSC), total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) antioxidant capacities of fenugreek seed extract. The levels of independent variables were first evaluated using one-factor-at-one-time method, after which the suitable levels (−1, 0, and +1) were selected for the optimization process. The optimal conditions of MAE parameters were found to be 2.84 min, 572.50 W, 63.68%, and 0.09 g/mL. Based on the suggested optimum conditions, the obtained responses were 195.89 ± 1.07 (mg diosgenin equivalent/g d.w.), 81.85 ± 0.61 (mg gallic acid equivalent/g d.w.), 92.86 ± 0.56%, and 95.85 ± 0.81%. Analysis of variance revealed that all the responses including TSC, TPC, DPPH, and ABTS antioxidant capacities were significantly influenced (P < 0.05) by irradiation time, solvent concentration, microwave oven power, and feed-to-solvent ratio. Hence, the results obtained from liquid chromatography–quadrupole–time-of-flight mass spectrometry indicated the presence of steroid saponins, triterpenoid saponins, phenolics, and other natural antioxidants. Elsevier Masson SAS 2019 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/26647/1/Optimization%20of%20saponins1.pdf Akbari, Sweeta and Nour, A. H. and R. M., Yunus (2019) Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool. Comptes Rendus Chimie. pp. 1-14. ISSN 1631-0748. (In Press / Online First) (In Press / Online First) https://doi.org/10.1016/j.crci.2019.07.007 https://doi.org/10.1016/j.crci.2019.07.007 |
| spellingShingle | TP Chemical technology Akbari, Sweeta Nour, A. H. R. M., Yunus Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool |
| title | Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool |
| title_full | Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool |
| title_fullStr | Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool |
| title_full_unstemmed | Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool |
| title_short | Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool |
| title_sort | optimization of saponins phenolics and antioxidants extracted from fenugreek seeds using microwave assisted extraction and response surface methodology as an optimizing tool |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/26647/1/Optimization%20of%20saponins1.pdf |
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