Identification of bioactive phytochemicals using GC-Mass and TLC to the estimation of antimicrobial susceptibility of plant extracts

This study aims to evaluate the constituent compounds of plant extracts and their antimicrobial activity. Four different ethnomedicinal plant extracts including Piper nigrum, Nigella sativa, Cinnamomum zeylanicum, and Elettaria cardamomum were tested for antimicrobial susceptibility profile identifi...

Full description

Bibliographic Details
Main Authors: Ali, Muna Jalal, Makky, Essam A., Zareen, Seema, M. M., Yusoff
Format: Conference or Workshop Item
Language:English
Published: IOP Publishing 2019
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/26939/1/Identification%20of%20bioactive%20phytochemicals%20using%20GC%E2%80%93Mass%20and%20TLC.pdf
_version_ 1796993779883835392
author Ali, Muna Jalal
Makky, Essam A.
Zareen, Seema
M. M., Yusoff
author_facet Ali, Muna Jalal
Makky, Essam A.
Zareen, Seema
M. M., Yusoff
author_sort Ali, Muna Jalal
collection UMP
description This study aims to evaluate the constituent compounds of plant extracts and their antimicrobial activity. Four different ethnomedicinal plant extracts including Piper nigrum, Nigella sativa, Cinnamomum zeylanicum, and Elettaria cardamomum were tested for antimicrobial susceptibility profile identified using GC-Mass and demonstrated TLC analysis. We found the combined action of ethanol plant extracts (alone) against oral isolates showed a synergistic effect profile up to 32.20% when combination A (Ci/Ca) was added. The stearic and palmitic acids were the major constituent compounds of plant extracts which was exhibited high antimicrobial susceptibility against the bacterial isolates. We conclude that the stearic and palmitic acids were major constituent compounds of plant extracts.
first_indexed 2024-03-06T12:38:36Z
format Conference or Workshop Item
id UMPir26939
institution Universiti Malaysia Pahang
language English
last_indexed 2024-03-06T12:38:36Z
publishDate 2019
publisher IOP Publishing
record_format dspace
spelling UMPir269392020-03-20T02:02:39Z http://umpir.ump.edu.my/id/eprint/26939/ Identification of bioactive phytochemicals using GC-Mass and TLC to the estimation of antimicrobial susceptibility of plant extracts Ali, Muna Jalal Makky, Essam A. Zareen, Seema M. M., Yusoff QK Botany TP Chemical technology This study aims to evaluate the constituent compounds of plant extracts and their antimicrobial activity. Four different ethnomedicinal plant extracts including Piper nigrum, Nigella sativa, Cinnamomum zeylanicum, and Elettaria cardamomum were tested for antimicrobial susceptibility profile identified using GC-Mass and demonstrated TLC analysis. We found the combined action of ethanol plant extracts (alone) against oral isolates showed a synergistic effect profile up to 32.20% when combination A (Ci/Ca) was added. The stearic and palmitic acids were the major constituent compounds of plant extracts which was exhibited high antimicrobial susceptibility against the bacterial isolates. We conclude that the stearic and palmitic acids were major constituent compounds of plant extracts. IOP Publishing 2019-10-25 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/26939/1/Identification%20of%20bioactive%20phytochemicals%20using%20GC%E2%80%93Mass%20and%20TLC.pdf Ali, Muna Jalal and Makky, Essam A. and Zareen, Seema and M. M., Yusoff (2019) Identification of bioactive phytochemicals using GC-Mass and TLC to the estimation of antimicrobial susceptibility of plant extracts. In: Journal of Physics: Conference Series, 2nd International Science Conference , 24-25 April 2019 , University of Al-Qadisiyah Diwaniyah, Iraq. pp. 1-11., 1294 (062013). ISSN 1742-6588 (print); 1742-6596 (online) https://doi.org/10.1088/1742-6596/1294/6/062013
spellingShingle QK Botany
TP Chemical technology
Ali, Muna Jalal
Makky, Essam A.
Zareen, Seema
M. M., Yusoff
Identification of bioactive phytochemicals using GC-Mass and TLC to the estimation of antimicrobial susceptibility of plant extracts
title Identification of bioactive phytochemicals using GC-Mass and TLC to the estimation of antimicrobial susceptibility of plant extracts
title_full Identification of bioactive phytochemicals using GC-Mass and TLC to the estimation of antimicrobial susceptibility of plant extracts
title_fullStr Identification of bioactive phytochemicals using GC-Mass and TLC to the estimation of antimicrobial susceptibility of plant extracts
title_full_unstemmed Identification of bioactive phytochemicals using GC-Mass and TLC to the estimation of antimicrobial susceptibility of plant extracts
title_short Identification of bioactive phytochemicals using GC-Mass and TLC to the estimation of antimicrobial susceptibility of plant extracts
title_sort identification of bioactive phytochemicals using gc mass and tlc to the estimation of antimicrobial susceptibility of plant extracts
topic QK Botany
TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/26939/1/Identification%20of%20bioactive%20phytochemicals%20using%20GC%E2%80%93Mass%20and%20TLC.pdf
work_keys_str_mv AT alimunajalal identificationofbioactivephytochemicalsusinggcmassandtlctotheestimationofantimicrobialsusceptibilityofplantextracts
AT makkyessama identificationofbioactivephytochemicalsusinggcmassandtlctotheestimationofantimicrobialsusceptibilityofplantextracts
AT zareenseema identificationofbioactivephytochemicalsusinggcmassandtlctotheestimationofantimicrobialsusceptibilityofplantextracts
AT mmyusoff identificationofbioactivephytochemicalsusinggcmassandtlctotheestimationofantimicrobialsusceptibilityofplantextracts