Laccase enzyme immobilization on ferromagnetic support material for oil palm empty fruit bunches (OPEFB) pretreatment

Rapid development of oil palm industry causes a lot of environmental issues due to the large number of waste products such as OPEFB. OPEFB is rich in lignocellulose which can be hydrolyzed to product sustainable biofuel, an alternative sources of energy. The production of biofuel from lignocellulose...

Full description

Bibliographic Details
Main Authors: Rashid, Shah Samiur, Huan, Hu Shian, Mohd Hasbi, Ab. Rahim, Sikder, Md. Belal Hossain, Sasi, Ahmed Abdulsalam H.
Format: Conference or Workshop Item
Language:English
Published: IOP Publishing 2019
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/26950/1/Laccase%20enzyme%20immobilization%20on%20ferromagnetic%20support%20material%20for%20oil.pdf
_version_ 1825813075085754368
author Rashid, Shah Samiur
Huan, Hu Shian
Mohd Hasbi, Ab. Rahim
Sikder, Md. Belal Hossain
Sasi, Ahmed Abdulsalam H.
author_facet Rashid, Shah Samiur
Huan, Hu Shian
Mohd Hasbi, Ab. Rahim
Sikder, Md. Belal Hossain
Sasi, Ahmed Abdulsalam H.
author_sort Rashid, Shah Samiur
collection UMP
description Rapid development of oil palm industry causes a lot of environmental issues due to the large number of waste products such as OPEFB. OPEFB is rich in lignocellulose which can be hydrolyzed to product sustainable biofuel, an alternative sources of energy. The production of biofuel from lignocellulose waste requires pretreatment process to enhance efficiency of saccharification of the sugar content of the lignocellulose waste. Enzymatic pretreatment technique is more desirable as this technique does not generate greenhouse gas or any inhibitor that can interferes the saccharification process. Enzyme immobilization technique also involved to increase the stability of the enzyme and promote reutilization of the enzyme. In this study, laccase enzyme was immobilized on ferromagnetic particles to promote easy separation of the immobilized laccase enzymes from the reaction media. The result shows that laccase enzyme immobilized on the nickel particles which encapsulated with silica. 0.235 IU/g of immobilized laccase degraded 58 % of the lignin content in 0.5 g of OPEFB after 24 hours. Pretreated OPEFB increase the efficiency of saccharification by 161% in compare with the untreated OPEFB.
first_indexed 2024-03-06T12:38:38Z
format Conference or Workshop Item
id UMPir26950
institution Universiti Malaysia Pahang
language English
last_indexed 2024-03-06T12:38:38Z
publishDate 2019
publisher IOP Publishing
record_format dspace
spelling UMPir269502020-03-20T02:44:06Z http://umpir.ump.edu.my/id/eprint/26950/ Laccase enzyme immobilization on ferromagnetic support material for oil palm empty fruit bunches (OPEFB) pretreatment Rashid, Shah Samiur Huan, Hu Shian Mohd Hasbi, Ab. Rahim Sikder, Md. Belal Hossain Sasi, Ahmed Abdulsalam H. QD Chemistry TP Chemical technology Rapid development of oil palm industry causes a lot of environmental issues due to the large number of waste products such as OPEFB. OPEFB is rich in lignocellulose which can be hydrolyzed to product sustainable biofuel, an alternative sources of energy. The production of biofuel from lignocellulose waste requires pretreatment process to enhance efficiency of saccharification of the sugar content of the lignocellulose waste. Enzymatic pretreatment technique is more desirable as this technique does not generate greenhouse gas or any inhibitor that can interferes the saccharification process. Enzyme immobilization technique also involved to increase the stability of the enzyme and promote reutilization of the enzyme. In this study, laccase enzyme was immobilized on ferromagnetic particles to promote easy separation of the immobilized laccase enzymes from the reaction media. The result shows that laccase enzyme immobilized on the nickel particles which encapsulated with silica. 0.235 IU/g of immobilized laccase degraded 58 % of the lignin content in 0.5 g of OPEFB after 24 hours. Pretreated OPEFB increase the efficiency of saccharification by 161% in compare with the untreated OPEFB. IOP Publishing 2019-10-18 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/26950/1/Laccase%20enzyme%20immobilization%20on%20ferromagnetic%20support%20material%20for%20oil.pdf Rashid, Shah Samiur and Huan, Hu Shian and Mohd Hasbi, Ab. Rahim and Sikder, Md. Belal Hossain and Sasi, Ahmed Abdulsalam H. (2019) Laccase enzyme immobilization on ferromagnetic support material for oil palm empty fruit bunches (OPEFB) pretreatment. In: IOP Conference Series: Materials Science and Engineering, 4th International Conference on Design and Manufacturing Engineering , 15-17 July 2019 , Australia. pp. 1-6., 627 (012025). ISSN 1757-8981 (Print), 1757-899X (Online) (Published) https://doi.org/10.1088/1757-899X/627/1/012025
spellingShingle QD Chemistry
TP Chemical technology
Rashid, Shah Samiur
Huan, Hu Shian
Mohd Hasbi, Ab. Rahim
Sikder, Md. Belal Hossain
Sasi, Ahmed Abdulsalam H.
Laccase enzyme immobilization on ferromagnetic support material for oil palm empty fruit bunches (OPEFB) pretreatment
title Laccase enzyme immobilization on ferromagnetic support material for oil palm empty fruit bunches (OPEFB) pretreatment
title_full Laccase enzyme immobilization on ferromagnetic support material for oil palm empty fruit bunches (OPEFB) pretreatment
title_fullStr Laccase enzyme immobilization on ferromagnetic support material for oil palm empty fruit bunches (OPEFB) pretreatment
title_full_unstemmed Laccase enzyme immobilization on ferromagnetic support material for oil palm empty fruit bunches (OPEFB) pretreatment
title_short Laccase enzyme immobilization on ferromagnetic support material for oil palm empty fruit bunches (OPEFB) pretreatment
title_sort laccase enzyme immobilization on ferromagnetic support material for oil palm empty fruit bunches opefb pretreatment
topic QD Chemistry
TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/26950/1/Laccase%20enzyme%20immobilization%20on%20ferromagnetic%20support%20material%20for%20oil.pdf
work_keys_str_mv AT rashidshahsamiur laccaseenzymeimmobilizationonferromagneticsupportmaterialforoilpalmemptyfruitbunchesopefbpretreatment
AT huanhushian laccaseenzymeimmobilizationonferromagneticsupportmaterialforoilpalmemptyfruitbunchesopefbpretreatment
AT mohdhasbiabrahim laccaseenzymeimmobilizationonferromagneticsupportmaterialforoilpalmemptyfruitbunchesopefbpretreatment
AT sikdermdbelalhossain laccaseenzymeimmobilizationonferromagneticsupportmaterialforoilpalmemptyfruitbunchesopefbpretreatment
AT sasiahmedabdulsalamh laccaseenzymeimmobilizationonferromagneticsupportmaterialforoilpalmemptyfruitbunchesopefbpretreatment