Uric acid detection using uv-vis spectrometer

The aim of this research is to detect uric acid (UA) concentration using Ultraviolet-Visible (UV-Vis) spectrometer in the Ultraviolet (UV) region. Absorption technique was proposed to detect different uric acid concentrations and its UV absorption wavelength. Current practices commonly take a lot of...

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Main Authors: Nor Mazlee, Norazmi, Zul Rasyied, Abdul Rasat, Mazuina, Mohamad, Hadi, Manap
Format: Conference or Workshop Item
Language:English
Published: IOP Publishing 2017
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/27261/1/2.%20Uric%20acid%20detection%20using%20UV-Vis%20%20spectrometer.pdf
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author Nor Mazlee, Norazmi
Zul Rasyied, Abdul Rasat
Mazuina, Mohamad
Hadi, Manap
author_facet Nor Mazlee, Norazmi
Zul Rasyied, Abdul Rasat
Mazuina, Mohamad
Hadi, Manap
author_sort Nor Mazlee, Norazmi
collection UMP
description The aim of this research is to detect uric acid (UA) concentration using Ultraviolet-Visible (UV-Vis) spectrometer in the Ultraviolet (UV) region. Absorption technique was proposed to detect different uric acid concentrations and its UV absorption wavelength. Current practices commonly take a lot of times or require complicated structures for the detection process. By this proposed spectroscopic technique, every concentration can be detected and interpreted into an absorbance value at a constant wavelength peak in the UV region. This is due to the chemical characteristics belong to the uric acid since it has a particular absorption cross-section, σ which can be calculated using Beer’s Lambert law formula. The detection performance was displayed using Spectrasuite sofware. It showed fast time response about 3 seconds. The experiment proved that the concentrations of uric acid were successfully detected using UV-Vis pectrometer at a constant absorption UV wavelength, 294.46 nm in a low time response. Even by an artificial sample of uric acid, it successfully displayed a close value as the ones reported with the use of the medical sample. It is applicable in the medical field and can be implemented in the future for earlier detection of abnormal concentration of uric acid.
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spelling UMPir272612020-01-16T03:20:27Z http://umpir.ump.edu.my/id/eprint/27261/ Uric acid detection using uv-vis spectrometer Nor Mazlee, Norazmi Zul Rasyied, Abdul Rasat Mazuina, Mohamad Hadi, Manap T Technology (General) The aim of this research is to detect uric acid (UA) concentration using Ultraviolet-Visible (UV-Vis) spectrometer in the Ultraviolet (UV) region. Absorption technique was proposed to detect different uric acid concentrations and its UV absorption wavelength. Current practices commonly take a lot of times or require complicated structures for the detection process. By this proposed spectroscopic technique, every concentration can be detected and interpreted into an absorbance value at a constant wavelength peak in the UV region. This is due to the chemical characteristics belong to the uric acid since it has a particular absorption cross-section, σ which can be calculated using Beer’s Lambert law formula. The detection performance was displayed using Spectrasuite sofware. It showed fast time response about 3 seconds. The experiment proved that the concentrations of uric acid were successfully detected using UV-Vis pectrometer at a constant absorption UV wavelength, 294.46 nm in a low time response. Even by an artificial sample of uric acid, it successfully displayed a close value as the ones reported with the use of the medical sample. It is applicable in the medical field and can be implemented in the future for earlier detection of abnormal concentration of uric acid. IOP Publishing 2017 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/27261/1/2.%20Uric%20acid%20detection%20using%20UV-Vis%20%20spectrometer.pdf Nor Mazlee, Norazmi and Zul Rasyied, Abdul Rasat and Mazuina, Mohamad and Hadi, Manap (2017) Uric acid detection using uv-vis spectrometer. In: IOP Conference Series: Materials Science and Engineering, 4th International Conference on Mechanical Engineering Research (ICMER2017) , 1-2 August 2017 , Kuantan, Pahang, Malaysia. pp. 1-6., 257 (012031). ISSN 1757-899X https://doi.org/10.1088/1757-899X/257/1/012031
spellingShingle T Technology (General)
Nor Mazlee, Norazmi
Zul Rasyied, Abdul Rasat
Mazuina, Mohamad
Hadi, Manap
Uric acid detection using uv-vis spectrometer
title Uric acid detection using uv-vis spectrometer
title_full Uric acid detection using uv-vis spectrometer
title_fullStr Uric acid detection using uv-vis spectrometer
title_full_unstemmed Uric acid detection using uv-vis spectrometer
title_short Uric acid detection using uv-vis spectrometer
title_sort uric acid detection using uv vis spectrometer
topic T Technology (General)
url http://umpir.ump.edu.my/id/eprint/27261/1/2.%20Uric%20acid%20detection%20using%20UV-Vis%20%20spectrometer.pdf
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AT mazuinamohamad uricaciddetectionusinguvvisspectrometer
AT hadimanap uricaciddetectionusinguvvisspectrometer