Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2
Fruit bromelain is a proteolytic enzyme harbouring cysteine catalytic residue found abundantly in pineapple fruit. The expression of cysteine proteases is usually regulated during fruit ripening. In the present study, we aimed to study the expression and proteolytic activity level of fruit bromelain...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Trans Tech Publications
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/28150/1/Gene%20Expression%20Analysis%20of%20Fruit%20Bromelain1.pdf |
| _version_ | 1796994025071312896 |
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| author | Wei, Cheng Pang Aizi Nor Mazila, Ramli Azzmer Azzar, Abdul Hamid |
| author_facet | Wei, Cheng Pang Aizi Nor Mazila, Ramli Azzmer Azzar, Abdul Hamid |
| author_sort | Wei, Cheng Pang |
| collection | UMP |
| description | Fruit bromelain is a proteolytic enzyme harbouring cysteine catalytic residue found abundantly in pineapple fruit. The expression of cysteine proteases is usually regulated during fruit ripening. In the present study, we aimed to study the expression and proteolytic activity level of fruit bromelain during the ripening stage of A. comosus cultivar MD 2. The gene expression of fruit bromelain was investigated via relative gene expression analysis using qPCR while the proteolytic activity of fruit bromelain was analysed via enzymatic assay using casein as a substrate. The qPCR analysis revealed that the expression of fruit bromelain was down-regulated 10-fold in ripe pineapple fruits. Besides that, the unripe pineapple fruits [1.9101 ± 0.0831 U/mL] had a higher proteolytic activity than the ripe MD 2 pineapple fruits [1.1333 ± 0.0896 U/mL]. This result showed that the function of fruit bromelain may be related to the protection of young pineapple fruits during the fruit development stage |
| first_indexed | 2024-03-06T12:41:57Z |
| format | Article |
| id | UMPir28150 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:41:57Z |
| publishDate | 2020 |
| publisher | Trans Tech Publications |
| record_format | dspace |
| spelling | UMPir281502020-03-18T01:44:05Z http://umpir.ump.edu.my/id/eprint/28150/ Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2 Wei, Cheng Pang Aizi Nor Mazila, Ramli Azzmer Azzar, Abdul Hamid QD Chemistry Fruit bromelain is a proteolytic enzyme harbouring cysteine catalytic residue found abundantly in pineapple fruit. The expression of cysteine proteases is usually regulated during fruit ripening. In the present study, we aimed to study the expression and proteolytic activity level of fruit bromelain during the ripening stage of A. comosus cultivar MD 2. The gene expression of fruit bromelain was investigated via relative gene expression analysis using qPCR while the proteolytic activity of fruit bromelain was analysed via enzymatic assay using casein as a substrate. The qPCR analysis revealed that the expression of fruit bromelain was down-regulated 10-fold in ripe pineapple fruits. Besides that, the unripe pineapple fruits [1.9101 ± 0.0831 U/mL] had a higher proteolytic activity than the ripe MD 2 pineapple fruits [1.1333 ± 0.0896 U/mL]. This result showed that the function of fruit bromelain may be related to the protection of young pineapple fruits during the fruit development stage Trans Tech Publications 2020 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/28150/1/Gene%20Expression%20Analysis%20of%20Fruit%20Bromelain1.pdf Wei, Cheng Pang and Aizi Nor Mazila, Ramli and Azzmer Azzar, Abdul Hamid (2020) Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2. Materials Science Forum, 981. pp. 209-214. ISSN 0255-5476. (Published) https://doi.org/10.4028/www.scientific.net/MSF.981.209 https://doi.org/10.4028/www.scientific.net/MSF.981.209 |
| spellingShingle | QD Chemistry Wei, Cheng Pang Aizi Nor Mazila, Ramli Azzmer Azzar, Abdul Hamid Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2 |
| title | Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2 |
| title_full | Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2 |
| title_fullStr | Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2 |
| title_full_unstemmed | Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2 |
| title_short | Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2 |
| title_sort | gene expression analysis of fruit bromelain in ripening of ananas comosus cultivar md 2 |
| topic | QD Chemistry |
| url | http://umpir.ump.edu.my/id/eprint/28150/1/Gene%20Expression%20Analysis%20of%20Fruit%20Bromelain1.pdf |
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