Factorial experimental design for xylanase production by Bacillus sp. isolated from Malaysia landfill soil
Two-level full factorial design was applied to screen the important parameters for production of xylanase by newly isolated Bacillus sp. from landfill soil. Five production parameters were considered: initial pH media (pH 5–9), inoculum size (5%–10% v/v), incubation period (18–30 h), temperature (30...
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| Fformat: | Conference or Workshop Item |
| Iaith: | English |
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IOP Publishing
2019
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| Pynciau: | |
| Mynediad Ar-lein: | http://umpir.ump.edu.my/id/eprint/28277/1/Factorial%20experimental%20design%20for%20xylanase.pdf |
| _version_ | 1825813324509478912 |
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| author | Siti Nor Amira, Rosli Rohaida, Che Man N., Masngut |
| author_facet | Siti Nor Amira, Rosli Rohaida, Che Man N., Masngut |
| author_sort | Siti Nor Amira, Rosli |
| collection | UMP |
| description | Two-level full factorial design was applied to screen the important parameters for production of xylanase by newly isolated Bacillus sp. from landfill soil. Five production parameters were considered: initial pH media (pH 5–9), inoculum size (5%–10% v/v), incubation period (18–30 h), temperature (30-50 °C) and agitation speed (0-200 rpm). Xylanase activity was estimated using dinitrosalicylic acid (DNS) based on the xylose released under specified assay conditions. Based on the factorial analysis, it was observed that the significant parameters in the xylanase production were temperature, agitation speed and initial pH of media. Meanwhile, the interaction between temperature and initial pH of media gave the highest influenced to the xylanase production. The model revealed that the highest xylanase activity can be achieved at 123.34 U/mL with initial pH media of 7.0, 30 h incubation period, 5% (v/v) inoculum size, agitation speed of 100 rpm at 40 °C. Confirmation run produced the highest experimental xylanase activity by Bacillus sp. at 123.10 U/mL with 0.17% of error than the predicted one. Hence, the model was reliably predicting the xylanase production. |
| first_indexed | 2024-03-06T12:42:17Z |
| format | Conference or Workshop Item |
| id | UMPir28277 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:42:17Z |
| publishDate | 2019 |
| publisher | IOP Publishing |
| record_format | dspace |
| spelling | UMPir282772020-04-30T03:52:19Z http://umpir.ump.edu.my/id/eprint/28277/ Factorial experimental design for xylanase production by Bacillus sp. isolated from Malaysia landfill soil Siti Nor Amira, Rosli Rohaida, Che Man N., Masngut TP Chemical technology Two-level full factorial design was applied to screen the important parameters for production of xylanase by newly isolated Bacillus sp. from landfill soil. Five production parameters were considered: initial pH media (pH 5–9), inoculum size (5%–10% v/v), incubation period (18–30 h), temperature (30-50 °C) and agitation speed (0-200 rpm). Xylanase activity was estimated using dinitrosalicylic acid (DNS) based on the xylose released under specified assay conditions. Based on the factorial analysis, it was observed that the significant parameters in the xylanase production were temperature, agitation speed and initial pH of media. Meanwhile, the interaction between temperature and initial pH of media gave the highest influenced to the xylanase production. The model revealed that the highest xylanase activity can be achieved at 123.34 U/mL with initial pH media of 7.0, 30 h incubation period, 5% (v/v) inoculum size, agitation speed of 100 rpm at 40 °C. Confirmation run produced the highest experimental xylanase activity by Bacillus sp. at 123.10 U/mL with 0.17% of error than the predicted one. Hence, the model was reliably predicting the xylanase production. IOP Publishing 2019 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/28277/1/Factorial%20experimental%20design%20for%20xylanase.pdf Siti Nor Amira, Rosli and Rohaida, Che Man and N., Masngut (2019) Factorial experimental design for xylanase production by Bacillus sp. isolated from Malaysia landfill soil. In: IOP Conference Series: Materials Science and Engineering, Energy Security and Chemical Engineering Congress , 17-19 July 2019 , Kuala Lumpur, Malaysia. pp. 1-10., 736 (022074). ISSN 1757-899X (Published) https://doi.org/10.1088/1757-899X/736/2/022074 |
| spellingShingle | TP Chemical technology Siti Nor Amira, Rosli Rohaida, Che Man N., Masngut Factorial experimental design for xylanase production by Bacillus sp. isolated from Malaysia landfill soil |
| title | Factorial experimental design for xylanase production by Bacillus sp. isolated from Malaysia landfill soil |
| title_full | Factorial experimental design for xylanase production by Bacillus sp. isolated from Malaysia landfill soil |
| title_fullStr | Factorial experimental design for xylanase production by Bacillus sp. isolated from Malaysia landfill soil |
| title_full_unstemmed | Factorial experimental design for xylanase production by Bacillus sp. isolated from Malaysia landfill soil |
| title_short | Factorial experimental design for xylanase production by Bacillus sp. isolated from Malaysia landfill soil |
| title_sort | factorial experimental design for xylanase production by bacillus sp isolated from malaysia landfill soil |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/28277/1/Factorial%20experimental%20design%20for%20xylanase.pdf |
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