Application of Michaelis-Menten in the kinetics of oil palm frond enzymatic hydrolysis for ferulic acid production
Ferulic acid (FA) production has become a frequent focus on today’s research due to its antioxidant properties. However, there has been little to none studies reported on the usage of mixed culture as inoculum in FA production. This study aims to determine the kinetics FA production in a mixed cultu...
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| Format: | Article |
| Language: | English English |
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Springer Nature
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/28409/1/39.%20Application%20of%20michaelis-menten%20in%20the%20kinetics%20of%20oil%20palm.pdf http://umpir.ump.edu.my/id/eprint/28409/2/39.1%20Application%20of%20michaelis-menten%20in%20the%20kinetics%20of%20oil%20palm.pdf |
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| author | Zulsyazwan, Ahmad Khushairi Kamaliah, Abdul Samad Nurul Aliaa, Abdul Rahman Hafizuddin, Wan Yussof Norazwina, Zainol |
| author_facet | Zulsyazwan, Ahmad Khushairi Kamaliah, Abdul Samad Nurul Aliaa, Abdul Rahman Hafizuddin, Wan Yussof Norazwina, Zainol |
| author_sort | Zulsyazwan, Ahmad Khushairi |
| collection | UMP |
| description | Ferulic acid (FA) production has become a frequent focus on today’s research due to its antioxidant properties. However, there has been little to none studies reported on the usage of mixed culture as inoculum in FA production. This study aims to determine the kinetics FA production in a mixed culture fermentation by applying the Michaelis-Menten kinetic model. In this study, mixed culture fermentation process using oil palm frond bagasse (OPFB) as substrate was applied for FA production improvement. Optimum condition was applied to study ferulic acid esterase (FAE) mechanism for kinetic modelling purposes. The kinetic model used was based on the Michaelis-Menten kinetic model. Runge-Kutta Fourth Order method was used to solve the kinetic model. Maximum FAE activity was achieved at the 28 h of fermentation process at 3.7 × 10-3 mU mL-1. This result proved that enzymatic hydrolysis occurred during fermentation process. Kinetic study was conducted with Michaelis-Menten kinetic model used as a reference kinetic equation. Three kinetic constants, Vmax, Km and Ks were determined as 3.725 × 10-3 g L-1 h-1, 28.231 g L-1 and 1.3 × 10-2 h-1 respectively using Runge-Kutta Fourth Order approach. The outcome of this study confirms that the kinetics of the process fit well with the Michaelis-Menten model. This also suggests that the usage of mixed culture has the potential to induce enzymatic hydrolysis hence improving FA production from OPFB during fermentation process. |
| first_indexed | 2024-03-06T12:42:39Z |
| format | Article |
| id | UMPir28409 |
| institution | Universiti Malaysia Pahang |
| language | English English |
| last_indexed | 2024-03-06T12:42:39Z |
| publishDate | 2020 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | UMPir284092020-06-11T04:06:32Z http://umpir.ump.edu.my/id/eprint/28409/ Application of Michaelis-Menten in the kinetics of oil palm frond enzymatic hydrolysis for ferulic acid production Zulsyazwan, Ahmad Khushairi Kamaliah, Abdul Samad Nurul Aliaa, Abdul Rahman Hafizuddin, Wan Yussof Norazwina, Zainol TP Chemical technology Ferulic acid (FA) production has become a frequent focus on today’s research due to its antioxidant properties. However, there has been little to none studies reported on the usage of mixed culture as inoculum in FA production. This study aims to determine the kinetics FA production in a mixed culture fermentation by applying the Michaelis-Menten kinetic model. In this study, mixed culture fermentation process using oil palm frond bagasse (OPFB) as substrate was applied for FA production improvement. Optimum condition was applied to study ferulic acid esterase (FAE) mechanism for kinetic modelling purposes. The kinetic model used was based on the Michaelis-Menten kinetic model. Runge-Kutta Fourth Order method was used to solve the kinetic model. Maximum FAE activity was achieved at the 28 h of fermentation process at 3.7 × 10-3 mU mL-1. This result proved that enzymatic hydrolysis occurred during fermentation process. Kinetic study was conducted with Michaelis-Menten kinetic model used as a reference kinetic equation. Three kinetic constants, Vmax, Km and Ks were determined as 3.725 × 10-3 g L-1 h-1, 28.231 g L-1 and 1.3 × 10-2 h-1 respectively using Runge-Kutta Fourth Order approach. The outcome of this study confirms that the kinetics of the process fit well with the Michaelis-Menten model. This also suggests that the usage of mixed culture has the potential to induce enzymatic hydrolysis hence improving FA production from OPFB during fermentation process. Springer Nature 2020 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/28409/1/39.%20Application%20of%20michaelis-menten%20in%20the%20kinetics%20of%20oil%20palm.pdf pdf en http://umpir.ump.edu.my/id/eprint/28409/2/39.1%20Application%20of%20michaelis-menten%20in%20the%20kinetics%20of%20oil%20palm.pdf Zulsyazwan, Ahmad Khushairi and Kamaliah, Abdul Samad and Nurul Aliaa, Abdul Rahman and Hafizuddin, Wan Yussof and Norazwina, Zainol (2020) Application of Michaelis-Menten in the kinetics of oil palm frond enzymatic hydrolysis for ferulic acid production. SN Applied Sciences, 2 (254). pp. 1-11. ISSN 2523-3963 (Print); 2523-3971 (Online). (Published) https://doi.org/10.1007/s42452-020-2062-3 https://doi.org/10.1007/s42452-020-2062-3 |
| spellingShingle | TP Chemical technology Zulsyazwan, Ahmad Khushairi Kamaliah, Abdul Samad Nurul Aliaa, Abdul Rahman Hafizuddin, Wan Yussof Norazwina, Zainol Application of Michaelis-Menten in the kinetics of oil palm frond enzymatic hydrolysis for ferulic acid production |
| title | Application of Michaelis-Menten in the kinetics of oil palm frond enzymatic hydrolysis for ferulic acid production |
| title_full | Application of Michaelis-Menten in the kinetics of oil palm frond enzymatic hydrolysis for ferulic acid production |
| title_fullStr | Application of Michaelis-Menten in the kinetics of oil palm frond enzymatic hydrolysis for ferulic acid production |
| title_full_unstemmed | Application of Michaelis-Menten in the kinetics of oil palm frond enzymatic hydrolysis for ferulic acid production |
| title_short | Application of Michaelis-Menten in the kinetics of oil palm frond enzymatic hydrolysis for ferulic acid production |
| title_sort | application of michaelis menten in the kinetics of oil palm frond enzymatic hydrolysis for ferulic acid production |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/28409/1/39.%20Application%20of%20michaelis-menten%20in%20the%20kinetics%20of%20oil%20palm.pdf http://umpir.ump.edu.my/id/eprint/28409/2/39.1%20Application%20of%20michaelis-menten%20in%20the%20kinetics%20of%20oil%20palm.pdf |
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