Evaluating the Effect of Combined Plant Extracts on α-amylase and α-glucosidase Inhibition Activity as Antidiabetic Agents
Background and Objective: Diabetes mellitus (DM) is the major health and economic problem through the world. The insufficiencies and severe adversarial special effects related with conventional medicines, led to a strong-minded search for alternative natural antidiabetic agents. The study was aimed...
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| Format: | Article |
| Language: | English |
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Science Alert
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/28734/1/Evaluating%20the%20Effect%20of%20Combined%20Plant.pdf |
| _version_ | 1796994129712906240 |
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| author | Munem, Osama A. Makky, Essam A. Tawfiq, Arwa A. Mahendran, Kasturi Normaiza, Z. |
| author_facet | Munem, Osama A. Makky, Essam A. Tawfiq, Arwa A. Mahendran, Kasturi Normaiza, Z. |
| author_sort | Munem, Osama A. |
| collection | UMP |
| description | Background and Objective: Diabetes mellitus (DM) is the major health and economic problem through the world. The insufficiencies and severe adversarial special effects related with conventional medicines, led to a strong-minded search for alternative natural antidiabetic agents. The study was aimed to screen the in vitro antidiabetic activity of seven plant extracts. Materials and Methods: The bioactivity of the extracts was correlated through a Gas Chromatography-Mass spectrometry (GC-MS)-based analysis, while extracts with 50% inhibition activity were reported. Results: The α-amylase inhibitory activities of the chloroform extracts of Averrhoa bilimbi, Andrographis paniculata, Eurycoma longifolia and Punica granatum exhibited more than 50% inhibition (50.55, 68.24, 82.42 and 69.73% respectively), while chloroform extracts of A. paniculata, Orthosiphon stamineus and P. granatum exhibited 90.48, 52.00 and 62.00% of α-glucosidase inhibitory effects, respectively. Only ethanolic and methanolic extracts of E. longifolia exhibited α-glucosidase inhibitory effects of 57.80 and 53.31%, respectively. A combination of the ethanolic fraction of E. longifolia with the chloroform fraction of P. granatum at a ratio of 2:1 exhibited the highest α-glucosidase inhibition rate of 148.06%, while a combination of the ethanolic fraction of E. longifolia with the chloroform fraction of O. stamineus at the same ratio exhibited α-glucosidase inhibition rate of 137.43%. A phytochemical analysis based on GC-MS revealed the presence of fatty acids, with palmitic acid recording the highest percentage (100%) in the O. stamineus extract. Conclusion: The observed α-amylase and α-glucosidase inhibitory activities of these plant extracts suggested their potential usage as an alternative sources of antidiabetic agents. |
| first_indexed | 2024-03-06T12:43:32Z |
| format | Article |
| id | UMPir28734 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:43:32Z |
| publishDate | 2020 |
| publisher | Science Alert |
| record_format | dspace |
| spelling | UMPir287342020-07-09T06:30:11Z http://umpir.ump.edu.my/id/eprint/28734/ Evaluating the Effect of Combined Plant Extracts on α-amylase and α-glucosidase Inhibition Activity as Antidiabetic Agents Munem, Osama A. Makky, Essam A. Tawfiq, Arwa A. Mahendran, Kasturi Normaiza, Z. QD Chemistry Background and Objective: Diabetes mellitus (DM) is the major health and economic problem through the world. The insufficiencies and severe adversarial special effects related with conventional medicines, led to a strong-minded search for alternative natural antidiabetic agents. The study was aimed to screen the in vitro antidiabetic activity of seven plant extracts. Materials and Methods: The bioactivity of the extracts was correlated through a Gas Chromatography-Mass spectrometry (GC-MS)-based analysis, while extracts with 50% inhibition activity were reported. Results: The α-amylase inhibitory activities of the chloroform extracts of Averrhoa bilimbi, Andrographis paniculata, Eurycoma longifolia and Punica granatum exhibited more than 50% inhibition (50.55, 68.24, 82.42 and 69.73% respectively), while chloroform extracts of A. paniculata, Orthosiphon stamineus and P. granatum exhibited 90.48, 52.00 and 62.00% of α-glucosidase inhibitory effects, respectively. Only ethanolic and methanolic extracts of E. longifolia exhibited α-glucosidase inhibitory effects of 57.80 and 53.31%, respectively. A combination of the ethanolic fraction of E. longifolia with the chloroform fraction of P. granatum at a ratio of 2:1 exhibited the highest α-glucosidase inhibition rate of 148.06%, while a combination of the ethanolic fraction of E. longifolia with the chloroform fraction of O. stamineus at the same ratio exhibited α-glucosidase inhibition rate of 137.43%. A phytochemical analysis based on GC-MS revealed the presence of fatty acids, with palmitic acid recording the highest percentage (100%) in the O. stamineus extract. Conclusion: The observed α-amylase and α-glucosidase inhibitory activities of these plant extracts suggested their potential usage as an alternative sources of antidiabetic agents. Science Alert 2020 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/28734/1/Evaluating%20the%20Effect%20of%20Combined%20Plant.pdf Munem, Osama A. and Makky, Essam A. and Tawfiq, Arwa A. and Mahendran, Kasturi and Normaiza, Z. (2020) Evaluating the Effect of Combined Plant Extracts on α-amylase and α-glucosidase Inhibition Activity as Antidiabetic Agents. Pakistan Journal of Biological Sciences, 23 (3). pp. 287-294. ISSN 1028-8880. (Published) http://dx.doi.org/10.3923/pjbs.2020.287.294 http://dx.doi.org/10.3923/pjbs.2020.287.294 |
| spellingShingle | QD Chemistry Munem, Osama A. Makky, Essam A. Tawfiq, Arwa A. Mahendran, Kasturi Normaiza, Z. Evaluating the Effect of Combined Plant Extracts on α-amylase and α-glucosidase Inhibition Activity as Antidiabetic Agents |
| title | Evaluating the Effect of Combined Plant Extracts on α-amylase and α-glucosidase Inhibition Activity as Antidiabetic Agents |
| title_full | Evaluating the Effect of Combined Plant Extracts on α-amylase and α-glucosidase Inhibition Activity as Antidiabetic Agents |
| title_fullStr | Evaluating the Effect of Combined Plant Extracts on α-amylase and α-glucosidase Inhibition Activity as Antidiabetic Agents |
| title_full_unstemmed | Evaluating the Effect of Combined Plant Extracts on α-amylase and α-glucosidase Inhibition Activity as Antidiabetic Agents |
| title_short | Evaluating the Effect of Combined Plant Extracts on α-amylase and α-glucosidase Inhibition Activity as Antidiabetic Agents |
| title_sort | evaluating the effect of combined plant extracts on α amylase and α glucosidase inhibition activity as antidiabetic agents |
| topic | QD Chemistry |
| url | http://umpir.ump.edu.my/id/eprint/28734/1/Evaluating%20the%20Effect%20of%20Combined%20Plant.pdf |
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