Development of paper based amperometric biosensor for glucose content measurement in Malaysian Stingless Bee Honey
Amperometric biosensor for glucose content measurement in Malaysian stingless bee honey was developed using screen printed carbon electrode (SPCE) integrated with paper disc immobilized with enzyme Glucose Oxidase (GOx) using simple physical adsorption method. The paper-based biosensor required only...
| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
IOP Publishing
2020
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/29371/1/33.%20Development%20of%20paper%20based%20amperometric%20biosensor%20for%20glucose%20content.pdf |
| _version_ | 1796994231987863552 |
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| author | W. N., Julika Azilah, Ajit N., Syazlin A., Ariff A., Naila A. Z., Sulaiman |
| author_facet | W. N., Julika Azilah, Ajit N., Syazlin A., Ariff A., Naila A. Z., Sulaiman |
| author_sort | W. N., Julika |
| collection | UMP |
| description | Amperometric biosensor for glucose content measurement in Malaysian stingless bee honey was developed using screen printed carbon electrode (SPCE) integrated with paper disc immobilized with enzyme Glucose Oxidase (GOx) using simple physical adsorption method. The paper-based biosensor required only 8 µL of sample solution for glucose analysis. The calibration of glucose biosensor is linear between 0.5 mM to 4.5 mM (R2= 0.9925) and has a detection limit of 0.15 mM. Interference study on several compound affecting the biosensor response and storage stability was investigated. In addition, its performance was demonstrated in the analysis of six honey samples. The results obtained using glucose biosensor was validated by high performance liquid chromatographic (HPLC) method. The addition of glucose in pure honey at various concentration were also tested by this paper-based biosensor where the current obtained shows increasing trend with the addition of glucose. From this research, it can be concluded that, the prototype sensor to determine glucose adulteration in stingless bee honey was successfully developed. |
| first_indexed | 2024-03-06T12:45:02Z |
| format | Conference or Workshop Item |
| id | UMPir29371 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:45:02Z |
| publishDate | 2020 |
| publisher | IOP Publishing |
| record_format | dspace |
| spelling | UMPir293712022-06-10T04:04:51Z http://umpir.ump.edu.my/id/eprint/29371/ Development of paper based amperometric biosensor for glucose content measurement in Malaysian Stingless Bee Honey W. N., Julika Azilah, Ajit N., Syazlin A., Ariff A., Naila A. Z., Sulaiman TP Chemical technology Amperometric biosensor for glucose content measurement in Malaysian stingless bee honey was developed using screen printed carbon electrode (SPCE) integrated with paper disc immobilized with enzyme Glucose Oxidase (GOx) using simple physical adsorption method. The paper-based biosensor required only 8 µL of sample solution for glucose analysis. The calibration of glucose biosensor is linear between 0.5 mM to 4.5 mM (R2= 0.9925) and has a detection limit of 0.15 mM. Interference study on several compound affecting the biosensor response and storage stability was investigated. In addition, its performance was demonstrated in the analysis of six honey samples. The results obtained using glucose biosensor was validated by high performance liquid chromatographic (HPLC) method. The addition of glucose in pure honey at various concentration were also tested by this paper-based biosensor where the current obtained shows increasing trend with the addition of glucose. From this research, it can be concluded that, the prototype sensor to determine glucose adulteration in stingless bee honey was successfully developed. IOP Publishing 2020 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/29371/1/33.%20Development%20of%20paper%20based%20amperometric%20biosensor%20for%20glucose%20content.pdf W. N., Julika and Azilah, Ajit and N., Syazlin and A., Ariff and A., Naila and A. Z., Sulaiman (2020) Development of paper based amperometric biosensor for glucose content measurement in Malaysian Stingless Bee Honey. In: Journal of Physics: Conference Series, The 2nd Joint International Conference on Emerging Computing Technology and Sports (JICETS) , 25-27 November 2019 , Bandung, Indonesia. pp. 1-13., 1529 (052035). ISSN 1742-6588 (print); 1742-6596 (online) https://doi.org/10.1088/1742-6596/1529/5/052035 |
| spellingShingle | TP Chemical technology W. N., Julika Azilah, Ajit N., Syazlin A., Ariff A., Naila A. Z., Sulaiman Development of paper based amperometric biosensor for glucose content measurement in Malaysian Stingless Bee Honey |
| title | Development of paper based amperometric biosensor for glucose content measurement in Malaysian Stingless Bee Honey |
| title_full | Development of paper based amperometric biosensor for glucose content measurement in Malaysian Stingless Bee Honey |
| title_fullStr | Development of paper based amperometric biosensor for glucose content measurement in Malaysian Stingless Bee Honey |
| title_full_unstemmed | Development of paper based amperometric biosensor for glucose content measurement in Malaysian Stingless Bee Honey |
| title_short | Development of paper based amperometric biosensor for glucose content measurement in Malaysian Stingless Bee Honey |
| title_sort | development of paper based amperometric biosensor for glucose content measurement in malaysian stingless bee honey |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/29371/1/33.%20Development%20of%20paper%20based%20amperometric%20biosensor%20for%20glucose%20content.pdf |
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