An electrochemical DNA biosensor fabricated from graphene decorated with graphitic nanospheres
Graphene decorated with graphitic nanospheres functionalized with pyrene butyric acid (PBA) is used for the first time to fabricate a DNA biosensor. The electrode was formed by attaching a DNA probe onto PBA, which had been stacked onto a graphene material decorated with graphene nanospheres (GNSs)....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing Ltd
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/29914/1/An%20electrochemical%20DNA%20biosensor%20fabricated%20from%20graphene%20decorated.pdf |
| _version_ | 1796994338192883712 |
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| author | Raja Zaidatul Akhmar, Raja Jamaluddin Tan, Ling Ling Chong, Kwok Feng Heng, Lee Yook |
| author_facet | Raja Zaidatul Akhmar, Raja Jamaluddin Tan, Ling Ling Chong, Kwok Feng Heng, Lee Yook |
| author_sort | Raja Zaidatul Akhmar, Raja Jamaluddin |
| collection | UMP |
| description | Graphene decorated with graphitic nanospheres functionalized with pyrene butyric acid (PBA) is used for the first time to fabricate a DNA biosensor. The electrode was formed by attaching a DNA probe onto PBA, which had been stacked onto a graphene material decorated with graphene nanospheres (GNSs). The nanomaterial was drop-coated onto a carbon screen-printed electrode (SPE) to create the GNS-PBA modified electrode (GNS-PBA/SPE). A simple method was used to produce GNS by annealing graphene oxide (GO) solution at high temperature. Field emission scanning electron micrographs confirmed the presence of a spherical shape of GNS with a diameter range of 40–80 nm. A stable and uniform PBA-modified GNS (GNS-PBA) was obtained with a facile ultrasonication step. Thus allowing aminated DNA probes of genetically modified (GM) soybean to be attached to the nanomaterials to form the DNA biosensor. The GNS-PBA/SPE exhibited excellent electrical conductivity via cyclic voltammetry (CV) and differential pulse voltammetry (DPV) tests using potassium ferricyanide (K3[Fe(CN)6]) as the electroactive probe. By employing an anthraquinone monosulfonic acid (AQMS) redox intercalator as the DNA hybridization indicator, the biosensor response was evaluated using the DPV electrochemical method. A good linear relationship between AQMS oxidation peak current and target DNA concentrations from 1.0 × 10−16 to 1.0 × 10−8 M with a limit of detection (LOD) of less than 1.0 × 10−16 M was obtained. Selectivity experiments revealed that the voltammetric GM DNA biosensor could discriminate complementary sequences of GM soybean from non-complementary sequences and hence good recoveries were obtained for real GM soybean sample analysis. The main advantage of using GNS is an improvement of the DNA biosensor analytical performance. |
| first_indexed | 2024-03-06T12:46:25Z |
| format | Article |
| id | UMPir29914 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:46:25Z |
| publishDate | 2020 |
| publisher | IOP Publishing Ltd |
| record_format | dspace |
| spelling | UMPir299142022-11-10T02:35:06Z http://umpir.ump.edu.my/id/eprint/29914/ An electrochemical DNA biosensor fabricated from graphene decorated with graphitic nanospheres Raja Zaidatul Akhmar, Raja Jamaluddin Tan, Ling Ling Chong, Kwok Feng Heng, Lee Yook TP Chemical technology Graphene decorated with graphitic nanospheres functionalized with pyrene butyric acid (PBA) is used for the first time to fabricate a DNA biosensor. The electrode was formed by attaching a DNA probe onto PBA, which had been stacked onto a graphene material decorated with graphene nanospheres (GNSs). The nanomaterial was drop-coated onto a carbon screen-printed electrode (SPE) to create the GNS-PBA modified electrode (GNS-PBA/SPE). A simple method was used to produce GNS by annealing graphene oxide (GO) solution at high temperature. Field emission scanning electron micrographs confirmed the presence of a spherical shape of GNS with a diameter range of 40–80 nm. A stable and uniform PBA-modified GNS (GNS-PBA) was obtained with a facile ultrasonication step. Thus allowing aminated DNA probes of genetically modified (GM) soybean to be attached to the nanomaterials to form the DNA biosensor. The GNS-PBA/SPE exhibited excellent electrical conductivity via cyclic voltammetry (CV) and differential pulse voltammetry (DPV) tests using potassium ferricyanide (K3[Fe(CN)6]) as the electroactive probe. By employing an anthraquinone monosulfonic acid (AQMS) redox intercalator as the DNA hybridization indicator, the biosensor response was evaluated using the DPV electrochemical method. A good linear relationship between AQMS oxidation peak current and target DNA concentrations from 1.0 × 10−16 to 1.0 × 10−8 M with a limit of detection (LOD) of less than 1.0 × 10−16 M was obtained. Selectivity experiments revealed that the voltammetric GM DNA biosensor could discriminate complementary sequences of GM soybean from non-complementary sequences and hence good recoveries were obtained for real GM soybean sample analysis. The main advantage of using GNS is an improvement of the DNA biosensor analytical performance. IOP Publishing Ltd 2020-09-09 Article PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/29914/1/An%20electrochemical%20DNA%20biosensor%20fabricated%20from%20graphene%20decorated.pdf Raja Zaidatul Akhmar, Raja Jamaluddin and Tan, Ling Ling and Chong, Kwok Feng and Heng, Lee Yook (2020) An electrochemical DNA biosensor fabricated from graphene decorated with graphitic nanospheres. Nanotechnology, 31 (48). pp. 1-12. ISSN 0957-4484. (Published) https://doi.org/10.1088/1361-6528/abab2e https://doi.org/10.1088/1361-6528/abab2e |
| spellingShingle | TP Chemical technology Raja Zaidatul Akhmar, Raja Jamaluddin Tan, Ling Ling Chong, Kwok Feng Heng, Lee Yook An electrochemical DNA biosensor fabricated from graphene decorated with graphitic nanospheres |
| title | An electrochemical DNA biosensor fabricated from graphene decorated with graphitic nanospheres |
| title_full | An electrochemical DNA biosensor fabricated from graphene decorated with graphitic nanospheres |
| title_fullStr | An electrochemical DNA biosensor fabricated from graphene decorated with graphitic nanospheres |
| title_full_unstemmed | An electrochemical DNA biosensor fabricated from graphene decorated with graphitic nanospheres |
| title_short | An electrochemical DNA biosensor fabricated from graphene decorated with graphitic nanospheres |
| title_sort | electrochemical dna biosensor fabricated from graphene decorated with graphitic nanospheres |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/29914/1/An%20electrochemical%20DNA%20biosensor%20fabricated%20from%20graphene%20decorated.pdf |
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