Molecular characterization of plasmid-mediated non-O157 verotoxigenic escherichia coli isolated from infants and children with diarrhea

A significant increase in the incidence of non-O157 verotoxigenic Escherichia coli (VTEC) infections have become a serious health issues, and this situation is worsening due to the dissemination of plasmid mediated multidrug-resistant microorganisms worldwide. This study aims to investigate the pres...

Full description

Bibliographic Details
Main Authors: Karim Khan, Md Fazlul, Rashid, Shah Samiur, Mohammad, Nazmul, Hassan, Maziz, Aizi Nor Mazila, Ramli, Roesnita, Baharuddin
Format: Article
Language:English
Published: University of Baghdad 2020
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/30501/1/Molecular%20characterization%20of%20plasmid-mediated%20non-O157%20verotoxigenic.pdf
_version_ 1796994440830648320
author Karim Khan, Md Fazlul
Rashid, Shah Samiur
Mohammad, Nazmul
Hassan, Maziz
Aizi Nor Mazila, Ramli
Roesnita, Baharuddin
author_facet Karim Khan, Md Fazlul
Rashid, Shah Samiur
Mohammad, Nazmul
Hassan, Maziz
Aizi Nor Mazila, Ramli
Roesnita, Baharuddin
author_sort Karim Khan, Md Fazlul
collection UMP
description A significant increase in the incidence of non-O157 verotoxigenic Escherichia coli (VTEC) infections have become a serious health issues, and this situation is worsening due to the dissemination of plasmid mediated multidrug-resistant microorganisms worldwide. This study aims to investigate the presence of plasmid-mediated verotoxin gene in non-O157 E. coli. Standard microbiological techniques identified a total of 137 E. coli isolates. The plasmid was detected by Perfectprep Plasmid Mini preparation kit. These isolates were subjected to disk diffusion assay, and plasmid curing with ethidium bromide treatment. The plasmid containing isolates were subjected to a polymerase chain reaction (PCR) for investigating the presence of plasmid mediated verotoxin gene (VT1 and VT2) in non-O157 E. coli. Among the 137 E. coli isolates, 49 isolates were non-O157 E. coli while 29 (59.1%) isolates were verotoxin producing non-O157 serotypes and 26 non-O157 VTEC isolates possessed plasmids. Certain isolates harboured single sized plasmid while others had multiple plasmids with different size varied from 1.8kb to 7.6kb. A plasmid containing all (100%) the isolates was multidrug-resistant. Eight isolates changed their susceptibility patterns while three isolates were found to lose plasmid after post plasmid curing treatment and the rest of the isolates (15) remained constant. Different PCR sets characterized 3 plasmid-mediated verotoxins producing non-O157 E. coli. This current study demonstrated the occurrence of plasmid mediated verotoxin gene in non-O157 E. coli. To the best of our knowledge, this is the first report in the global literature on plasmid-mediated verotoxin gene in non-O157 E. coli. Timely diagnosis and surveillance of VTEC infections should prioritize to stop or slow down the virulence gene for dissemination by plasmid-mediated gene transfer amongst the same bacteria or other species.
first_indexed 2024-03-06T12:47:50Z
format Article
id UMPir30501
institution Universiti Malaysia Pahang
language English
last_indexed 2024-03-06T12:47:50Z
publishDate 2020
publisher University of Baghdad
record_format dspace
spelling UMPir305012021-06-30T14:25:53Z http://umpir.ump.edu.my/id/eprint/30501/ Molecular characterization of plasmid-mediated non-O157 verotoxigenic escherichia coli isolated from infants and children with diarrhea Karim Khan, Md Fazlul Rashid, Shah Samiur Mohammad, Nazmul Hassan, Maziz Aizi Nor Mazila, Ramli Roesnita, Baharuddin QR Microbiology TP Chemical technology A significant increase in the incidence of non-O157 verotoxigenic Escherichia coli (VTEC) infections have become a serious health issues, and this situation is worsening due to the dissemination of plasmid mediated multidrug-resistant microorganisms worldwide. This study aims to investigate the presence of plasmid-mediated verotoxin gene in non-O157 E. coli. Standard microbiological techniques identified a total of 137 E. coli isolates. The plasmid was detected by Perfectprep Plasmid Mini preparation kit. These isolates were subjected to disk diffusion assay, and plasmid curing with ethidium bromide treatment. The plasmid containing isolates were subjected to a polymerase chain reaction (PCR) for investigating the presence of plasmid mediated verotoxin gene (VT1 and VT2) in non-O157 E. coli. Among the 137 E. coli isolates, 49 isolates were non-O157 E. coli while 29 (59.1%) isolates were verotoxin producing non-O157 serotypes and 26 non-O157 VTEC isolates possessed plasmids. Certain isolates harboured single sized plasmid while others had multiple plasmids with different size varied from 1.8kb to 7.6kb. A plasmid containing all (100%) the isolates was multidrug-resistant. Eight isolates changed their susceptibility patterns while three isolates were found to lose plasmid after post plasmid curing treatment and the rest of the isolates (15) remained constant. Different PCR sets characterized 3 plasmid-mediated verotoxins producing non-O157 E. coli. This current study demonstrated the occurrence of plasmid mediated verotoxin gene in non-O157 E. coli. To the best of our knowledge, this is the first report in the global literature on plasmid-mediated verotoxin gene in non-O157 E. coli. Timely diagnosis and surveillance of VTEC infections should prioritize to stop or slow down the virulence gene for dissemination by plasmid-mediated gene transfer amongst the same bacteria or other species. University of Baghdad 2020-09-01 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/30501/1/Molecular%20characterization%20of%20plasmid-mediated%20non-O157%20verotoxigenic.pdf Karim Khan, Md Fazlul and Rashid, Shah Samiur and Mohammad, Nazmul and Hassan, Maziz and Aizi Nor Mazila, Ramli and Roesnita, Baharuddin (2020) Molecular characterization of plasmid-mediated non-O157 verotoxigenic escherichia coli isolated from infants and children with diarrhea. Baghdad Science Journal, 13 (3). pp. 710-719. ISSN 2078-8665. (Published) https://doi.org/10.21123/bsj.2020.17.3.0710 https://doi.org/10.21123/bsj.2020.17.3.0710
spellingShingle QR Microbiology
TP Chemical technology
Karim Khan, Md Fazlul
Rashid, Shah Samiur
Mohammad, Nazmul
Hassan, Maziz
Aizi Nor Mazila, Ramli
Roesnita, Baharuddin
Molecular characterization of plasmid-mediated non-O157 verotoxigenic escherichia coli isolated from infants and children with diarrhea
title Molecular characterization of plasmid-mediated non-O157 verotoxigenic escherichia coli isolated from infants and children with diarrhea
title_full Molecular characterization of plasmid-mediated non-O157 verotoxigenic escherichia coli isolated from infants and children with diarrhea
title_fullStr Molecular characterization of plasmid-mediated non-O157 verotoxigenic escherichia coli isolated from infants and children with diarrhea
title_full_unstemmed Molecular characterization of plasmid-mediated non-O157 verotoxigenic escherichia coli isolated from infants and children with diarrhea
title_short Molecular characterization of plasmid-mediated non-O157 verotoxigenic escherichia coli isolated from infants and children with diarrhea
title_sort molecular characterization of plasmid mediated non o157 verotoxigenic escherichia coli isolated from infants and children with diarrhea
topic QR Microbiology
TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/30501/1/Molecular%20characterization%20of%20plasmid-mediated%20non-O157%20verotoxigenic.pdf
work_keys_str_mv AT karimkhanmdfazlul molecularcharacterizationofplasmidmediatednono157verotoxigenicescherichiacoliisolatedfrominfantsandchildrenwithdiarrhea
AT rashidshahsamiur molecularcharacterizationofplasmidmediatednono157verotoxigenicescherichiacoliisolatedfrominfantsandchildrenwithdiarrhea
AT mohammadnazmul molecularcharacterizationofplasmidmediatednono157verotoxigenicescherichiacoliisolatedfrominfantsandchildrenwithdiarrhea
AT hassanmaziz molecularcharacterizationofplasmidmediatednono157verotoxigenicescherichiacoliisolatedfrominfantsandchildrenwithdiarrhea
AT aizinormazilaramli molecularcharacterizationofplasmidmediatednono157verotoxigenicescherichiacoliisolatedfrominfantsandchildrenwithdiarrhea
AT roesnitabaharuddin molecularcharacterizationofplasmidmediatednono157verotoxigenicescherichiacoliisolatedfrominfantsandchildrenwithdiarrhea