Characterization of gel beads used for immobilization of chlorella vulgaris and enhancement its density for lipid production

The success of microalgae immobilized in gel beads for lipid production heavily relies on the characteristics of the matrices employed. This study aims to determine cell density and characteristics of immobilized Chlorella vulgaris cells that contributed to the viability of the cells within beads. T...

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Main Authors: N. R., Abu Sepian, Nur Hidayah, Mat Yasin, Norazwina, Zainol
Format: Conference or Workshop Item
Language:English
Published: IOP Publishing 2020
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/30918/1/Characterization%20of%20gel%20beads%20used%20for%20immobilization%20of%20chlorella.pdf
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author N. R., Abu Sepian
Nur Hidayah, Mat Yasin
Norazwina, Zainol
author_facet N. R., Abu Sepian
Nur Hidayah, Mat Yasin
Norazwina, Zainol
author_sort N. R., Abu Sepian
collection UMP
description The success of microalgae immobilized in gel beads for lipid production heavily relies on the characteristics of the matrices employed. This study aims to determine cell density and characteristics of immobilized Chlorella vulgaris cells that contributed to the viability of the cells within beads. The new combined matrices with volumetric ratios (Matrices:Microalgae) namely, (1) mixed matrices, SACMCCA (0.3:1) (2) SACA (0.3:1) (3) SACMC (1:1) were proposed in this study and (4) SA (1:1) (sodium alginate, SA; calcium alginate, CA; sodium carboxymethyl cellulose, CMC) as a control experiment. The size of the beads, membrane thickness and chemical compound of these beads were examined. The cell density demonstrated that SACMCCA beads presented the highest value of 1.72 ± 0.5 × 109 cells/mL and lipid yield (30.43 ± 0.30 %) compared to SACA (24.29 ± 0.50 %), SACMC (13.00 ± 0.60 %) and SA (6.71 ± 0.50 %). The characterization had provided important characteristics for a successful entrapment of Chlorella vulgaris. In addition, the combination of single matrices had improved the cell density and lipid production for future applications in the biofuel industry.
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spelling UMPir309182021-07-26T13:56:17Z http://umpir.ump.edu.my/id/eprint/30918/ Characterization of gel beads used for immobilization of chlorella vulgaris and enhancement its density for lipid production N. R., Abu Sepian Nur Hidayah, Mat Yasin Norazwina, Zainol TP Chemical technology The success of microalgae immobilized in gel beads for lipid production heavily relies on the characteristics of the matrices employed. This study aims to determine cell density and characteristics of immobilized Chlorella vulgaris cells that contributed to the viability of the cells within beads. The new combined matrices with volumetric ratios (Matrices:Microalgae) namely, (1) mixed matrices, SACMCCA (0.3:1) (2) SACA (0.3:1) (3) SACMC (1:1) were proposed in this study and (4) SA (1:1) (sodium alginate, SA; calcium alginate, CA; sodium carboxymethyl cellulose, CMC) as a control experiment. The size of the beads, membrane thickness and chemical compound of these beads were examined. The cell density demonstrated that SACMCCA beads presented the highest value of 1.72 ± 0.5 × 109 cells/mL and lipid yield (30.43 ± 0.30 %) compared to SACA (24.29 ± 0.50 %), SACMC (13.00 ± 0.60 %) and SA (6.71 ± 0.50 %). The characterization had provided important characteristics for a successful entrapment of Chlorella vulgaris. In addition, the combination of single matrices had improved the cell density and lipid production for future applications in the biofuel industry. IOP Publishing 2020-12-22 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/30918/1/Characterization%20of%20gel%20beads%20used%20for%20immobilization%20of%20chlorella.pdf N. R., Abu Sepian and Nur Hidayah, Mat Yasin and Norazwina, Zainol (2020) Characterization of gel beads used for immobilization of chlorella vulgaris and enhancement its density for lipid production. In: IOP Conference Series: Materials Science and Engineering; 5th International Conference of Chemical Engineering and Industrial Biotechnology, ICCEIB 2020 , 9 - 11 August 2020 , Kuala Lumpur, Malaysia. pp. 1-13., 991 (1). ISSN 1757-8981 (Print), 1757-899X (Online) (Published) https://doi.org/10.1088/1757-899X/991/1/012007
spellingShingle TP Chemical technology
N. R., Abu Sepian
Nur Hidayah, Mat Yasin
Norazwina, Zainol
Characterization of gel beads used for immobilization of chlorella vulgaris and enhancement its density for lipid production
title Characterization of gel beads used for immobilization of chlorella vulgaris and enhancement its density for lipid production
title_full Characterization of gel beads used for immobilization of chlorella vulgaris and enhancement its density for lipid production
title_fullStr Characterization of gel beads used for immobilization of chlorella vulgaris and enhancement its density for lipid production
title_full_unstemmed Characterization of gel beads used for immobilization of chlorella vulgaris and enhancement its density for lipid production
title_short Characterization of gel beads used for immobilization of chlorella vulgaris and enhancement its density for lipid production
title_sort characterization of gel beads used for immobilization of chlorella vulgaris and enhancement its density for lipid production
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/30918/1/Characterization%20of%20gel%20beads%20used%20for%20immobilization%20of%20chlorella.pdf
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