Evaluation of the antioxidant and antimicrobial activities of ethyl acetate extract of saccharomyces cerevisiae.

Research background. Antioxidants are described as important compounds that are present at low concentrations to inhibit oxidation processes. Due to the side effects of synthetic antioxidants, research interest has increased considerably towards finding natural sources of antioxidants that can repla...

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Main Authors: Makky, Essam A., Manaf, Almatar, Mahmood, Mahmood H.
Format: Article
Language:English
English
Published: PubMed 2021
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/31564/3/Evaluation%20of%20the%20Antioxidant%20and%20Antimicrobial%20Activities%20of%20Ethyl%20Acetate%20Extract%20of%20Saccharomyces%20cerevisiae.pdf
http://umpir.ump.edu.my/id/eprint/31564/4/1706-evaluation-of-the-antioxidant-and-antimicrobial-activities-of-ethyl-acetate-extract-of-saccharomyces-cerevisiae
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author Makky, Essam A.
Manaf, Almatar
Mahmood, Mahmood H.
author_facet Makky, Essam A.
Manaf, Almatar
Mahmood, Mahmood H.
author_sort Makky, Essam A.
collection UMP
description Research background. Antioxidants are described as important compounds that are present at low concentrations to inhibit oxidation processes. Due to the side effects of synthetic antioxidants, research interest has increased considerably towards finding natural sources of antioxidants that can replace synthetic antioxidants. The emergence and spread of antibiotic resistance require the development of new drugs or some potential sources of novel medicine. This work aims to extract the secondary metabolites of Saccharomyces cerevisiae using ethyl acetate as a solvent and to determine the antioxidant and antimicrobial activities of these extracted metabolites. Experimental approach. The antioxidant activities of the secondary metabolites of S. cerevisiae were determined using DPPH, ABTS, and FRAP assays. Furthermore, the antimicrobial potential of the ethyl acetate extract of S. cerevisiae in treating Cutibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis was assessed. Results and conclusions. Five out of 13 of the extracted secondary metabolites were identified as antioxidants. The antioxidant activity of the S. cerevisiae extract exhibited relatively high IC50 of 455.2689 and 294.51 μg/mL for DPPH and ABTS respectively while the FRAP value was obtained as 44.4004 μg AAE/mL. Moreover, the extracts presented a significant antibacterial activity (p<0.05) against Staphylococcus aureus and Staphylococcus epidermidis at the concentrations of 100 and 200 mg/mL, respectively. However, no inhibitory effect was observed against Cutibacterium acnes as the extract was only effective against Cutibacterium acnes at the concentrations of 300 and 400 mg/mL (inhibition zones ranging from 9.0±0 to 9.333±0.577) respectively (p<0.05). Staphylococcus aureus was highly sensitive to the extract, with a MIC value of 18.75 mg/mL. Novelty and scientific contribution. This report confirmed the efficacy of the secondary metabolites of S. cerevisiae as a natural source of antioxidants and antimicrobials and suggest the possibility of employing them in drugs for the treatment of infectious diseases caused by the tested microorganisms.
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spelling UMPir315642021-07-02T08:48:08Z http://umpir.ump.edu.my/id/eprint/31564/ Evaluation of the antioxidant and antimicrobial activities of ethyl acetate extract of saccharomyces cerevisiae. Makky, Essam A. Manaf, Almatar Mahmood, Mahmood H. QR Microbiology Research background. Antioxidants are described as important compounds that are present at low concentrations to inhibit oxidation processes. Due to the side effects of synthetic antioxidants, research interest has increased considerably towards finding natural sources of antioxidants that can replace synthetic antioxidants. The emergence and spread of antibiotic resistance require the development of new drugs or some potential sources of novel medicine. This work aims to extract the secondary metabolites of Saccharomyces cerevisiae using ethyl acetate as a solvent and to determine the antioxidant and antimicrobial activities of these extracted metabolites. Experimental approach. The antioxidant activities of the secondary metabolites of S. cerevisiae were determined using DPPH, ABTS, and FRAP assays. Furthermore, the antimicrobial potential of the ethyl acetate extract of S. cerevisiae in treating Cutibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis was assessed. Results and conclusions. Five out of 13 of the extracted secondary metabolites were identified as antioxidants. The antioxidant activity of the S. cerevisiae extract exhibited relatively high IC50 of 455.2689 and 294.51 μg/mL for DPPH and ABTS respectively while the FRAP value was obtained as 44.4004 μg AAE/mL. Moreover, the extracts presented a significant antibacterial activity (p<0.05) against Staphylococcus aureus and Staphylococcus epidermidis at the concentrations of 100 and 200 mg/mL, respectively. However, no inhibitory effect was observed against Cutibacterium acnes as the extract was only effective against Cutibacterium acnes at the concentrations of 300 and 400 mg/mL (inhibition zones ranging from 9.0±0 to 9.333±0.577) respectively (p<0.05). Staphylococcus aureus was highly sensitive to the extract, with a MIC value of 18.75 mg/mL. Novelty and scientific contribution. This report confirmed the efficacy of the secondary metabolites of S. cerevisiae as a natural source of antioxidants and antimicrobials and suggest the possibility of employing them in drugs for the treatment of infectious diseases caused by the tested microorganisms. PubMed 2021 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/31564/3/Evaluation%20of%20the%20Antioxidant%20and%20Antimicrobial%20Activities%20of%20Ethyl%20Acetate%20Extract%20of%20Saccharomyces%20cerevisiae.pdf text en http://umpir.ump.edu.my/id/eprint/31564/4/1706-evaluation-of-the-antioxidant-and-antimicrobial-activities-of-ethyl-acetate-extract-of-saccharomyces-cerevisiae Makky, Essam A. and Manaf, Almatar and Mahmood, Mahmood H. (2021) Evaluation of the antioxidant and antimicrobial activities of ethyl acetate extract of saccharomyces cerevisiae. Food Technology and Biotechnology, 59 (2). pp. 1-4. ISSN 1330-9862. (In Press / Online First) (In Press / Online First)
spellingShingle QR Microbiology
Makky, Essam A.
Manaf, Almatar
Mahmood, Mahmood H.
Evaluation of the antioxidant and antimicrobial activities of ethyl acetate extract of saccharomyces cerevisiae.
title Evaluation of the antioxidant and antimicrobial activities of ethyl acetate extract of saccharomyces cerevisiae.
title_full Evaluation of the antioxidant and antimicrobial activities of ethyl acetate extract of saccharomyces cerevisiae.
title_fullStr Evaluation of the antioxidant and antimicrobial activities of ethyl acetate extract of saccharomyces cerevisiae.
title_full_unstemmed Evaluation of the antioxidant and antimicrobial activities of ethyl acetate extract of saccharomyces cerevisiae.
title_short Evaluation of the antioxidant and antimicrobial activities of ethyl acetate extract of saccharomyces cerevisiae.
title_sort evaluation of the antioxidant and antimicrobial activities of ethyl acetate extract of saccharomyces cerevisiae
topic QR Microbiology
url http://umpir.ump.edu.my/id/eprint/31564/3/Evaluation%20of%20the%20Antioxidant%20and%20Antimicrobial%20Activities%20of%20Ethyl%20Acetate%20Extract%20of%20Saccharomyces%20cerevisiae.pdf
http://umpir.ump.edu.my/id/eprint/31564/4/1706-evaluation-of-the-antioxidant-and-antimicrobial-activities-of-ethyl-acetate-extract-of-saccharomyces-cerevisiae
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