Induction of apoptosis and regulation of MicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells
(2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) is a synthetic curcumin analogue, which has been reported to possess anti-tumor, anti-metastatic, and anti-invasion properties on estrogen receptor (ER) negative breast cancer cells in vitro and in vivo. However, the cytotoxic eff...
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| Format: | Article |
| Language: | English |
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MDPI - Open Access Publishing
2021
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| Online Access: | http://umpir.ump.edu.my/id/eprint/31831/1/Induction%20of%20apoptosis%20and%20regulation%20of%20microRNA%20expression.pdf |
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| author | Yeap, Swee Keong Norlaily, Mohd Ali Akhtar, Muhammad Nadeem Nursyamirah, Abd Razak Chong, Zhi Xiong Ho, Wan Yong Boo, Lily Seema, Zareen Tonni Agustiono, Kurniawan Avtar, Ram Ng, Stephanie Y. L. Ong, Alan Han Kiat Alitheen, Noorjahan Banu |
| author_facet | Yeap, Swee Keong Norlaily, Mohd Ali Akhtar, Muhammad Nadeem Nursyamirah, Abd Razak Chong, Zhi Xiong Ho, Wan Yong Boo, Lily Seema, Zareen Tonni Agustiono, Kurniawan Avtar, Ram Ng, Stephanie Y. L. Ong, Alan Han Kiat Alitheen, Noorjahan Banu |
| author_sort | Yeap, Swee Keong |
| collection | UMP |
| description | (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) is a synthetic curcumin analogue, which has been reported to possess anti-tumor, anti-metastatic, and anti-invasion properties on estrogen receptor (ER) negative breast cancer cells in vitro and in vivo. However, the cytotoxic effects of BHMC on ER positive breast cancer cells were not widely reported. This study was aimed to investigate the cytotoxic potential of BHMC on MCF-7 cells using cell viability, cell cycle, and apoptotic assays. Besides, microarray and quantitative polymerase chain reaction (qPCR) were performed to identify the list of miRNAs and genes, which could be dysregulated following BHMC treatment. The current study discovered that BHMC exhibits selective cytotoxic effects on ER positive MCF-7 cells as compared to ER negative MDA-MB-231 cells and normal breast cells, MCF-10A. BHMC was shown to promote G2/M cell cycle arrest and apoptosis in MCF-7 cells. Microarray and qPCR analysis demonstrated that BHMC treatment would upregulate several miRNAs like miR-3195 and miR-30a-3p and downregulate miRNAs such as miR-6813-5p and miR-6132 in MCF-7 cells. Besides, BHMC administration was also found to downregulate few tumor-promoting genes like VEGF and SNAIL in MCF-7. In conclusion, BHMC induced apoptosis in the MCF-7 cells by altering the expressions of apoptotic-regulating miRNAs and associated genes. |
| first_indexed | 2024-03-06T12:51:20Z |
| format | Article |
| id | UMPir31831 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:51:20Z |
| publishDate | 2021 |
| publisher | MDPI - Open Access Publishing |
| record_format | dspace |
| spelling | UMPir318312021-08-20T14:16:37Z http://umpir.ump.edu.my/id/eprint/31831/ Induction of apoptosis and regulation of MicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells Yeap, Swee Keong Norlaily, Mohd Ali Akhtar, Muhammad Nadeem Nursyamirah, Abd Razak Chong, Zhi Xiong Ho, Wan Yong Boo, Lily Seema, Zareen Tonni Agustiono, Kurniawan Avtar, Ram Ng, Stephanie Y. L. Ong, Alan Han Kiat Alitheen, Noorjahan Banu TP Chemical technology (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) is a synthetic curcumin analogue, which has been reported to possess anti-tumor, anti-metastatic, and anti-invasion properties on estrogen receptor (ER) negative breast cancer cells in vitro and in vivo. However, the cytotoxic effects of BHMC on ER positive breast cancer cells were not widely reported. This study was aimed to investigate the cytotoxic potential of BHMC on MCF-7 cells using cell viability, cell cycle, and apoptotic assays. Besides, microarray and quantitative polymerase chain reaction (qPCR) were performed to identify the list of miRNAs and genes, which could be dysregulated following BHMC treatment. The current study discovered that BHMC exhibits selective cytotoxic effects on ER positive MCF-7 cells as compared to ER negative MDA-MB-231 cells and normal breast cells, MCF-10A. BHMC was shown to promote G2/M cell cycle arrest and apoptosis in MCF-7 cells. Microarray and qPCR analysis demonstrated that BHMC treatment would upregulate several miRNAs like miR-3195 and miR-30a-3p and downregulate miRNAs such as miR-6813-5p and miR-6132 in MCF-7 cells. Besides, BHMC administration was also found to downregulate few tumor-promoting genes like VEGF and SNAIL in MCF-7. In conclusion, BHMC induced apoptosis in the MCF-7 cells by altering the expressions of apoptotic-regulating miRNAs and associated genes. MDPI - Open Access Publishing 2021-02-26 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/31831/1/Induction%20of%20apoptosis%20and%20regulation%20of%20microRNA%20expression.pdf Yeap, Swee Keong and Norlaily, Mohd Ali and Akhtar, Muhammad Nadeem and Nursyamirah, Abd Razak and Chong, Zhi Xiong and Ho, Wan Yong and Boo, Lily and Seema, Zareen and Tonni Agustiono, Kurniawan and Avtar, Ram and Ng, Stephanie Y. L. and Ong, Alan Han Kiat and Alitheen, Noorjahan Banu (2021) Induction of apoptosis and regulation of MicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells. Molecules, 26 (5). pp. 1-15. ISSN 1420-3049 (print); 1420-3049 (online). (Published) https://doi.org/10.3390/molecules26051277 https://doi.org/10.3390/molecules26051277 |
| spellingShingle | TP Chemical technology Yeap, Swee Keong Norlaily, Mohd Ali Akhtar, Muhammad Nadeem Nursyamirah, Abd Razak Chong, Zhi Xiong Ho, Wan Yong Boo, Lily Seema, Zareen Tonni Agustiono, Kurniawan Avtar, Ram Ng, Stephanie Y. L. Ong, Alan Han Kiat Alitheen, Noorjahan Banu Induction of apoptosis and regulation of MicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells |
| title | Induction of apoptosis and regulation of MicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells |
| title_full | Induction of apoptosis and regulation of MicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells |
| title_fullStr | Induction of apoptosis and regulation of MicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells |
| title_full_unstemmed | Induction of apoptosis and regulation of MicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells |
| title_short | Induction of apoptosis and regulation of MicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells |
| title_sort | induction of apoptosis and regulation of microrna expression by 2e 6e 2 6 bis 4 hydroxy 3 methoxybenzylidene cyclohexanone bhmc treatment on mcf 7 breast cancer cells |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/31831/1/Induction%20of%20apoptosis%20and%20regulation%20of%20microRNA%20expression.pdf |
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