| 总结: | Palm oil mill effluents is a waste that produce in high quantity in Malaysia. Furthur studies would futher increase profitability of palm oil industries besides solving environmental problems. POME has great potential as substrate because it has low cost and also it contains a mixture of carbohydrates binding, hemicelluloses, sucrose, and other carbohydrates that can be utilized by saccharolytic clostria.This research mainly to study the effect of temperature to the solventogenic fermentation using clostridia.acetobutylicum in producing butanol and ethanol by using palm oil mill effluent (POME) as a fermentation media. This research also to investigate the type of sugar that contain in POME, the profile growth rate of clostridia acetobutylicum and then to investigate consumption of glucose by clostridia acetobutylicum during fermentation. The experiment works were conducted in schott bottle in anaerobic chamber to maintain anaerobic condition. Fermentation was carried out for 72 hour at 35°C with the concentration of POME at 90% and inoculums concentration at 10%. Palm oil mill effluent and reinforced clostridia medium was used as a growth medium in batch culture. The temperature that used in this investigation is 33°C, 34°C, 35°C and 37°C. The result of this investigation showed that POME is a viable media for butanol and ethanol fermentation. The result also showed that the yield of butanol and ethanol production will decrease as the temperature increase. The results showed that the highest yield of butanol produced was 0.156 g/L. For ethanol production, the highest was 58.51 g/L. The experimental results also showed that the sugar groups that contains in POME are fructose, glucose, galactose, sucrose and lactose. From this study, it was observed that optimum condition for butanol and ethanol fermentation by Clostridia acetobutylicum at 35°C.
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