Structural analysis of plasmid-mediated verotoxin gene producing non-o157 escherichia coli using molecular dynamic simulations
Plasmid-mediated Non-O157 VTEC is a pathogenic E. coli serotype, are responsible for many life-threatening diseases such as diarrhoea. The thermostable crystal structure has been widely sought after for industry and therapeutic applications through the structural analysis. The National Center for Bi...
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| Format: | Article |
| Language: | English |
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Slovak University of Agriculture
2021
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| Online Access: | http://umpir.ump.edu.my/id/eprint/32778/1/Structural%20analysis%20of%20plasmid-mediated%20verotoxin%20gene%20producing%20non-o157%20escherichia%20coli.pdf |
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| author | Khan, M. F. K. Rashid, S. S. Ramli, A. N. M. Kabir, M. N. Maziz, M. N. H. |
| author_facet | Khan, M. F. K. Rashid, S. S. Ramli, A. N. M. Kabir, M. N. Maziz, M. N. H. |
| author_sort | Khan, M. F. K. |
| collection | UMP |
| description | Plasmid-mediated Non-O157 VTEC is a pathogenic E. coli serotype, are responsible for many life-threatening diseases such as diarrhoea. The thermostable crystal structure has been widely sought after for industry and therapeutic applications through the structural analysis. The National Center for Biotechnology Information (NCBI) Genbank Database has been sourced to obtain plasmid-mediated Verotoxin genes producing non-O157 MN 696158 (Vtx1-1) and MN688720 (Vtx2) sequences. The tertiary structure of MN696158 (Vtx1-1) and MN688720 (Vtx2) was generated by operating MODELLER. The result exposed multiple templates during modelling processes have improved the local stereochemical quality of the produced models. The structural analysis also disclosed the similarities and differences between the models (Vtx1-1 and Vtx2). Furthermore, the thermal stability profile of plasmid-mediated non-O157 VTEC were studied. The molecular dynamics simulations of plasmid-mediated non-O157 VTEC structures (Vtx1-1 and Vtx2) illustrated the interactions between amino acids. On the other hand, Vtx1-1 and Vtx2 showed noticeable differences in their relative conformational flexibility and stability at elevated temperature. However, it is expected that the information of the thermal stable of plasmid-mediated non-O157 VTEC models can be used for potential vaccine candidate through protein engineering in future. |
| first_indexed | 2024-03-06T12:53:51Z |
| format | Article |
| id | UMPir32778 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:53:51Z |
| publishDate | 2021 |
| publisher | Slovak University of Agriculture |
| record_format | dspace |
| spelling | UMPir327782022-03-08T03:17:53Z http://umpir.ump.edu.my/id/eprint/32778/ Structural analysis of plasmid-mediated verotoxin gene producing non-o157 escherichia coli using molecular dynamic simulations Khan, M. F. K. Rashid, S. S. Ramli, A. N. M. Kabir, M. N. Maziz, M. N. H. T Technology (General) TP Chemical technology Plasmid-mediated Non-O157 VTEC is a pathogenic E. coli serotype, are responsible for many life-threatening diseases such as diarrhoea. The thermostable crystal structure has been widely sought after for industry and therapeutic applications through the structural analysis. The National Center for Biotechnology Information (NCBI) Genbank Database has been sourced to obtain plasmid-mediated Verotoxin genes producing non-O157 MN 696158 (Vtx1-1) and MN688720 (Vtx2) sequences. The tertiary structure of MN696158 (Vtx1-1) and MN688720 (Vtx2) was generated by operating MODELLER. The result exposed multiple templates during modelling processes have improved the local stereochemical quality of the produced models. The structural analysis also disclosed the similarities and differences between the models (Vtx1-1 and Vtx2). Furthermore, the thermal stability profile of plasmid-mediated non-O157 VTEC were studied. The molecular dynamics simulations of plasmid-mediated non-O157 VTEC structures (Vtx1-1 and Vtx2) illustrated the interactions between amino acids. On the other hand, Vtx1-1 and Vtx2 showed noticeable differences in their relative conformational flexibility and stability at elevated temperature. However, it is expected that the information of the thermal stable of plasmid-mediated non-O157 VTEC models can be used for potential vaccine candidate through protein engineering in future. Slovak University of Agriculture 2021-08-01 Article PeerReviewed pdf en cc_by_nd_4 http://umpir.ump.edu.my/id/eprint/32778/1/Structural%20analysis%20of%20plasmid-mediated%20verotoxin%20gene%20producing%20non-o157%20escherichia%20coli.pdf Khan, M. F. K. and Rashid, S. S. and Ramli, A. N. M. and Kabir, M. N. and Maziz, M. N. H. (2021) Structural analysis of plasmid-mediated verotoxin gene producing non-o157 escherichia coli using molecular dynamic simulations. Journal of Microbiology, Biotechnology and Food Sciences, 11 (1). pp. 1-7. ISSN 1338-5178. (Published) https://doi.org/10.15414/jmbfs.4142 https://doi.org/10.15414/jmbfs.4142 |
| spellingShingle | T Technology (General) TP Chemical technology Khan, M. F. K. Rashid, S. S. Ramli, A. N. M. Kabir, M. N. Maziz, M. N. H. Structural analysis of plasmid-mediated verotoxin gene producing non-o157 escherichia coli using molecular dynamic simulations |
| title | Structural analysis of plasmid-mediated verotoxin gene producing non-o157 escherichia coli using molecular dynamic simulations |
| title_full | Structural analysis of plasmid-mediated verotoxin gene producing non-o157 escherichia coli using molecular dynamic simulations |
| title_fullStr | Structural analysis of plasmid-mediated verotoxin gene producing non-o157 escherichia coli using molecular dynamic simulations |
| title_full_unstemmed | Structural analysis of plasmid-mediated verotoxin gene producing non-o157 escherichia coli using molecular dynamic simulations |
| title_short | Structural analysis of plasmid-mediated verotoxin gene producing non-o157 escherichia coli using molecular dynamic simulations |
| title_sort | structural analysis of plasmid mediated verotoxin gene producing non o157 escherichia coli using molecular dynamic simulations |
| topic | T Technology (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/32778/1/Structural%20analysis%20of%20plasmid-mediated%20verotoxin%20gene%20producing%20non-o157%20escherichia%20coli.pdf |
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