Whey protein fraction based on Q-sepharose anion exchange chromatography
Whey proteinsarewell known for their nutritional value and had versatile functional properties.The mains proteins present in whey are α-lactalbumin, β-lactoglobulin, immunoglobulin andbovin eserum albumin. In this study, whey protein component is separate into several fractions using Q-Sepharose ani...
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| Format: | Undergraduates Project Papers |
| Language: | English |
| Published: |
2010
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| Online Access: | http://umpir.ump.edu.my/id/eprint/3283/1/CD5505_ZIANOR_AZLINA.pdf |
| _version_ | 1825821368957009920 |
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| author | Zianor Azrina, Z. A. |
| author_facet | Zianor Azrina, Z. A. |
| author_sort | Zianor Azrina, Z. A. |
| collection | UMP |
| description | Whey proteinsarewell known for their nutritional value and had versatile functional properties.The mains proteins present in whey are α-lactalbumin, β-lactoglobulin, immunoglobulin andbovin eserum albumin. In this study, whey protein component is separate into several fractions using Q-Sepharose anion exchange chromatography column. The chromatographic process was run at different pH range from pH5 to pH8. The best separation was achieved at pH6 in which the major protein component can be separated into their individual protein fraction especially contains α-
lactalbumin and β-lactoglobulin. The yield of α-lactalbumin and β-lactoglobulin recovered from 2 ml whey at pH 6 was 46% and 98.4% respectively. Fractionation of single and pure protein component from whey can add the value of the wheybased product and these proteins can be used for specific application. |
| first_indexed | 2024-03-06T11:40:29Z |
| format | Undergraduates Project Papers |
| id | UMPir3283 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T11:40:29Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | UMPir32832021-07-01T07:55:33Z http://umpir.ump.edu.my/id/eprint/3283/ Whey protein fraction based on Q-sepharose anion exchange chromatography Zianor Azrina, Z. A. TP Chemical technology Whey proteinsarewell known for their nutritional value and had versatile functional properties.The mains proteins present in whey are α-lactalbumin, β-lactoglobulin, immunoglobulin andbovin eserum albumin. In this study, whey protein component is separate into several fractions using Q-Sepharose anion exchange chromatography column. The chromatographic process was run at different pH range from pH5 to pH8. The best separation was achieved at pH6 in which the major protein component can be separated into their individual protein fraction especially contains α- lactalbumin and β-lactoglobulin. The yield of α-lactalbumin and β-lactoglobulin recovered from 2 ml whey at pH 6 was 46% and 98.4% respectively. Fractionation of single and pure protein component from whey can add the value of the wheybased product and these proteins can be used for specific application. 2010-12 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3283/1/CD5505_ZIANOR_AZLINA.pdf Zianor Azrina, Z. A. (2010) Whey protein fraction based on Q-sepharose anion exchange chromatography. Faculty of Chemical & Natural Resources Engineering , Universiti Malaysia Pahang. |
| spellingShingle | TP Chemical technology Zianor Azrina, Z. A. Whey protein fraction based on Q-sepharose anion exchange chromatography |
| title | Whey protein fraction based on Q-sepharose anion exchange chromatography |
| title_full | Whey protein fraction based on Q-sepharose anion exchange chromatography |
| title_fullStr | Whey protein fraction based on Q-sepharose anion exchange chromatography |
| title_full_unstemmed | Whey protein fraction based on Q-sepharose anion exchange chromatography |
| title_short | Whey protein fraction based on Q-sepharose anion exchange chromatography |
| title_sort | whey protein fraction based on q sepharose anion exchange chromatography |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/3283/1/CD5505_ZIANOR_AZLINA.pdf |
| work_keys_str_mv | AT zianorazrinaza wheyproteinfractionbasedonqsepharoseanionexchangechromatography |