Cytotoxic activity of eurycoma longifolia jack root extract against nasopharyngeal carcinoma cell line
Background: Eurycoma longifolia (E. longifolia) or Tongkat Ali is a tree that grows in southeast Asia, the roots of which contain bioactive components that exhibit cytotoxic properties against various cancer cell lines. However, no study has been conducted to relate the cytotoxic properties against...
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| Format: | Article |
| Language: | English |
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EManuscript Technologies
2021
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| Online Access: | http://umpir.ump.edu.my/id/eprint/33131/1/Cytotoxic%20activity%20of%20eurycoma%20longifolia%20jack%20root%20extract%20against%20nasopharyngeal.pdf |
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| author | Nur Haseena, Kajahmohideen Siti NurSyafiqah, Razi Faisal, Ghasak Ghazi Ashour, Abdelkader Elbadawy Kusumawardani, Anisa Makky, Essam A. Ibrahim, O. E. |
| author_facet | Nur Haseena, Kajahmohideen Siti NurSyafiqah, Razi Faisal, Ghasak Ghazi Ashour, Abdelkader Elbadawy Kusumawardani, Anisa Makky, Essam A. Ibrahim, O. E. |
| author_sort | Nur Haseena, Kajahmohideen |
| collection | UMP |
| description | Background: Eurycoma longifolia (E. longifolia) or Tongkat Ali is a tree that grows in southeast Asia, the roots of which contain bioactive components that exhibit cytotoxic properties against various cancer cell lines. However, no study has been conducted to relate the cytotoxic properties against nasopharyngeal carcinoma (NPC), a type of cancer that shows poor prognosis for metastatic disease. The purpose of this study was to determine whether the E. longifolia root extract exerts cytotoxic activity against nasopharyngeal carcinoma (ORL-115) cell lines. Materials and Method: E. longifolia root extracts were obtained through Soxhlet extraction method and by using two different solvents; ethanol and dicholoromethane. MTS assay was used to evaluate the cytotoxic effect of the root extracts against ORL-115 cell line for three different incubation time which were 24-hour, 48-hour and 72-hour. Results: Ethanol extract was significantly more potent compared to DCM extract. Ethanol extract exhibited lower IC50 value compared to DCM extract. The IC50 of ethanol extract were 232.1 µg/ml, 66.86 µg/ml and 42.6 µg/ml. Meanwhile the IC50 of DCM extract were 678.87 µg/ml, 136.71 µg/ml, 73.72 µg/ml for 24-hour, 48-hour and 72-hour incubation period respectively. The cytotoxic activity of both extracts increased as the incubation time prolonged. The cytotoxic activity of ethanol extract at each incubation time was significantly different from DCM extract except at 72 hours. Conclusion: E. longifolia root extracts exerted cytotoxic activity against the nasopharyngeal carcinoma (ORL-115) cell line. Ethanol extract exhibited lower IC50 value compared to DCM extract. The cytotoxic activity of both extracts were dose dependent and time dependent. |
| first_indexed | 2024-03-06T12:54:39Z |
| format | Article |
| id | UMPir33131 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:54:39Z |
| publishDate | 2021 |
| publisher | EManuscript Technologies |
| record_format | dspace |
| spelling | UMPir331312022-06-16T04:16:06Z http://umpir.ump.edu.my/id/eprint/33131/ Cytotoxic activity of eurycoma longifolia jack root extract against nasopharyngeal carcinoma cell line Nur Haseena, Kajahmohideen Siti NurSyafiqah, Razi Faisal, Ghasak Ghazi Ashour, Abdelkader Elbadawy Kusumawardani, Anisa Makky, Essam A. Ibrahim, O. E. QK Botany T Technology (General) TP Chemical technology Background: Eurycoma longifolia (E. longifolia) or Tongkat Ali is a tree that grows in southeast Asia, the roots of which contain bioactive components that exhibit cytotoxic properties against various cancer cell lines. However, no study has been conducted to relate the cytotoxic properties against nasopharyngeal carcinoma (NPC), a type of cancer that shows poor prognosis for metastatic disease. The purpose of this study was to determine whether the E. longifolia root extract exerts cytotoxic activity against nasopharyngeal carcinoma (ORL-115) cell lines. Materials and Method: E. longifolia root extracts were obtained through Soxhlet extraction method and by using two different solvents; ethanol and dicholoromethane. MTS assay was used to evaluate the cytotoxic effect of the root extracts against ORL-115 cell line for three different incubation time which were 24-hour, 48-hour and 72-hour. Results: Ethanol extract was significantly more potent compared to DCM extract. Ethanol extract exhibited lower IC50 value compared to DCM extract. The IC50 of ethanol extract were 232.1 µg/ml, 66.86 µg/ml and 42.6 µg/ml. Meanwhile the IC50 of DCM extract were 678.87 µg/ml, 136.71 µg/ml, 73.72 µg/ml for 24-hour, 48-hour and 72-hour incubation period respectively. The cytotoxic activity of both extracts increased as the incubation time prolonged. The cytotoxic activity of ethanol extract at each incubation time was significantly different from DCM extract except at 72 hours. Conclusion: E. longifolia root extracts exerted cytotoxic activity against the nasopharyngeal carcinoma (ORL-115) cell line. Ethanol extract exhibited lower IC50 value compared to DCM extract. The cytotoxic activity of both extracts were dose dependent and time dependent. EManuscript Technologies 2021-07 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/33131/1/Cytotoxic%20activity%20of%20eurycoma%20longifolia%20jack%20root%20extract%20against%20nasopharyngeal.pdf Nur Haseena, Kajahmohideen and Siti NurSyafiqah, Razi and Faisal, Ghasak Ghazi and Ashour, Abdelkader Elbadawy and Kusumawardani, Anisa and Makky, Essam A. and Ibrahim, O. E. (2021) Cytotoxic activity of eurycoma longifolia jack root extract against nasopharyngeal carcinoma cell line. Pharmacognosy Journal, 13 (4). pp. 1014-1018. ISSN 0975-3575. (Published) https://doi.org/10.5530/pj.2021.13.131 https://doi.org/10.5530/pj.2021.13.131 |
| spellingShingle | QK Botany T Technology (General) TP Chemical technology Nur Haseena, Kajahmohideen Siti NurSyafiqah, Razi Faisal, Ghasak Ghazi Ashour, Abdelkader Elbadawy Kusumawardani, Anisa Makky, Essam A. Ibrahim, O. E. Cytotoxic activity of eurycoma longifolia jack root extract against nasopharyngeal carcinoma cell line |
| title | Cytotoxic activity of eurycoma longifolia jack root extract against nasopharyngeal carcinoma cell line |
| title_full | Cytotoxic activity of eurycoma longifolia jack root extract against nasopharyngeal carcinoma cell line |
| title_fullStr | Cytotoxic activity of eurycoma longifolia jack root extract against nasopharyngeal carcinoma cell line |
| title_full_unstemmed | Cytotoxic activity of eurycoma longifolia jack root extract against nasopharyngeal carcinoma cell line |
| title_short | Cytotoxic activity of eurycoma longifolia jack root extract against nasopharyngeal carcinoma cell line |
| title_sort | cytotoxic activity of eurycoma longifolia jack root extract against nasopharyngeal carcinoma cell line |
| topic | QK Botany T Technology (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/33131/1/Cytotoxic%20activity%20of%20eurycoma%20longifolia%20jack%20root%20extract%20against%20nasopharyngeal.pdf |
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